Hypothesized mechanisms of death in swimming: a systematic review.

BACKGROUND: The study aims to update the specific classification of mechanisms of death in swimming and to demonstrate these categories are reasonable, by analyzing more characteristics of death cases, evaluating the available evidence and determining their quality. METHODS: Original articles were queried from PubMed, Web of Science, Embase databases, Cochrane Library, and Scopus. Included studies, which were evaluated as level 4 evidence or higher according to the Oxford Centre for Evidence-Based Medicine, discussed hypothesized mechanisms of death in swimming. Parameters analyzed in this study included decedents' characteristics, outcome measures, findings, methodological index for non-randomized studies (MINORS), and critical evaluation of each study classified by death mechanism. RESULTS: A total of twenty-five studies were included for further analysis: fourteen were associated with cardiovascular diseases, two were about cerebrovascular diseases, two contained respiratory diseases, seven were about hazardous conditions and three contained other drownings, which provided evidence for mechanisms of death. CONCLUSIONS: It is found that cardiovascular disease is the main cause or contributing factor of death in swimming. Respiratory diseases and cerebrovascular diseases are difficult to be definitive mechanism categories due to insufficient evidence. Hazardous conditions appear to be one of the possible risk factors because there are more cases of deaths from unsafe environments in swimming, but further statistics and research are still needed to support this view. Our study may have important implications for developing potential prevention strategies for sports and exercise medicine. TRIAL REGISTRATION: PROSPERO ID (CRD42021267330). Registered Aug 13th 2021.

Fast treatment and recycling method of large-scale vegetable wastes.

With rapid development of vegetable industry in China, in process of refrigerated transportation and storage, large-scale abandoned vegetable wastes (VW) need to be urgently treated alone since they rot very fast and would pollute the environment seriously. Existing treatment projects generally regard VW as garbage with high content of water and adopt the process of squeeze and sewage treatment, which leads to not only high treatment costs but also great resource waste. Therefore, according to the composition and degradation characteristics of VW, a novel fast treatment and recycling method of VW was proposed in this paper. VW are first degraded with thermostatic anaerobic digestion (AD) and then the residues decompose rapidly with thermostatic aerobic digestion to meet the farmland application standard. To verify the feasibility of the method, the pressed VW water (PVW) and VW from the VW treatment plant were mixed and degraded in two 0.56 m3 digesters, and degraded substances were continuously measured in 30 days' mesophilic AD at 37 ± 1 °C. Subsequently, the biogas slurry (BS) produced by AD is decomposed by thermostatic aerobic aeration decomposition at 30 °C for 48 h to rapidly decompose. BS was confirmed to use safely for plants by germination index (GI) test. The results show that 96 % chemical oxygen demand (COD) from 15,711 mg/L to 1000 mg/L within 31 days and the GI of treated BS was 81.75 %. Besides, nutrient elements of N, P, and K keep good abundance, no heavy metals, pesticide residue, and hazardous substances were found. Other parameters were all lower than the BS placed for a half-year. VW are fast-treated and recycled with the new method, which provides a novel method for fast treatment and recycling of large-scale VW.

Comparison of clinical efficiency between intra-articular injection of platelet-rich plasma and hyaluronic acid for osteoarthritis: a meta-analysis of randomized controlled trials.

Background: Platelet-rich plasma (PRP) and hyaluronic acid (HA) are non-surgical treatments for osteoarthritis (OA), but the comparison of their efficiency is still inconclusive. Objectives: The objectives of this study were to compare the efficacy of PRP and HA in the treatment of OA by meta-analysis and to explore the effects of different injection times and leukocyte concentration on the efficacy of PRP. Design: Meta-analysis and subgroup analysis were conducted. The data were analyzed by Review Manager v5.4.1. Data sources and methods: Articles were retrieved and screened from PubMed, the Cochrane Library, Web of Science, and Embase. The outcome included the total Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), the visual analog scale (VAS), adverse events (AEs), the International Knee Documentation Committee (IKDC), and the satisfaction rate. Results: A total of 30 articles involving 2733 patients were included. The total WOMAC score and IKDC score of the PRP group were better than those of the HA group at the last follow-up time, while there was no significant difference in AEs, satisfaction rate, and VAS between the two groups. In our subgroup analysis, there was no significant difference between single-injection PRP and triple-injection PRP. Leukocyte-poor PRP (LP-PRP) was better than leukocyte-rich PRP (LR-PRP) in IKDC, but there was no significant difference between them in the other scores. Conclusions: In the treatment of OA, compared with HA, PRP performed better in the improvement of the patient's function. There was no significant difference in VAS and AEs between the two groups, and the safety was comparable. LP-PRP looked to be superior to LR-PRP in functional recovery, but there appeared to be no significant difference in pain relief between them. There was no significant difference between single PRP and triple PRP in the subgroup analysis.

Feasibility of annual dry anaerobic digestion temperature-controlled by solar energy in cold and arid areas.

In cold and arid areas, variations of ambient temperature not only lead to a large amount of heat loss from anaerobic digestion reactors but also greater challenges in the stable production of biogas. Common temperature-controlled methods of biogas production, such as coal combustion, electric heating, biogas combustion and so on, are expensive and high energy-consuming. Openly, solar energy is economical and suitable for stable biogas production. However, no pilot studies have yet shown the feasibility of controlling the temperature of annual biogas production with solar energy in cold and arid areas. This paper first theoretically analyzed the energy balance between evacuated tube solar collectors and anaerobic reactors. Then a biogas production system was developed in Lanzhou City, China, consisting mainly of a 3 m3 insulated anaerobic reactor and a solar collector with 30 sticks Φ58 × L1800mm evacuated tubes. Annual batch experiments have been carried out to test the feasibility of stable biogas production at a temperature-controlled by solar energy in cold and arid areas. The results show that dry anaerobic digestion with 20% total solid (TS) can start and operate smoothly even under the condition of low solar irradiation for 3-4 consecutive days. The system can run stability by anaerobic digestion at 26 ± 1 °C in winter and spring, by mesophilic (37 ± 1 °C) and thermophilic (52 ± 1 °C) anaerobic digestion in summer and autumn, which implies a highly efficient operation strategy for agricultural and animal husbandry wastes treatment. These theoretical and experimental results provide a scientific basis and engineering reference for the application of biogas production temperature-controlled by solar energy and have important value for the efficient and low-cost anaerobic digestion treatment of agricultural and animal husbandry wastes in cold and arid areas.

Single and combined effects of microplastics and cadmium on the cadmium accumulation, antioxidant defence and innate immunity of the discus fish (Symphysodon aequifasciatus).

Microplastics (MPs) have the potential to interact with the toxicity of other common environmental contaminants, such as heavy metals. Here, we investigated the impacts of polystyrene-MPs (32-40 µm), cadmium (Cd) and their combination on early juveniles of the discus fish (Symphysodon aequifasciatus) in relation to Cd accumulation, antioxidant defence and innate immunity. Animals were exposed to three concentrations of MPs (0, 50 or 500 µg L-1) crossed with two levels of Cd (0 or 50 µg L-1) for 30 days. Our findings showed that MPs and Cd had no adverse effects on growth and survival. Under exposure to Cd, however, accumulation of Cd in the body of fish decreased with increasing MP concentrations as supported by a reduced metallothionein content. The activities of superoxide dismutase and glutathione peroxidase increased with MPs but decreased with Cd. MPs, Cd or the mixture increased catalase activity, despite an antagonistic interaction between the two stressors. Glutathione levels increased when exposed to high MP concentrations but decreased when co-exposed to Cd. Malondialdehyde content was only influenced by MPs and increased with elevated MPs. MPs or Cd alone did not increase protein carboxyl content but showed a synergistic effect and increased content. MPs or Cd alone showed no effect on lysozyme activity but had a synergistic effect and activated activity. Activities of both acid phosphatase and alkaline phosphatase were enhanced by MPs, Cd or their mixture, although there was an antagonistic interaction between the two stressors. In contrast, MPs, Cd or their mixture decreased complement 3 content, despite an antagonistic interaction between the two stressors. Collectively, this study suggests that exposure to Cd led to reduced Cd accumulation in the presence of MPs. Nevertheless, co-exposure could induce severe oxidative stress and stimulate innate immunity in the juvenile S. aequifasciatus.

Physiological responses to cold stress in the gills of discus fish (Symphysodon aequifasciatus) revealed by conventional biochemical assays and GC-TOF-MS metabolomics.

Discus fish (Symphysodon aequifasciatus) is a cichlid that is among the most popular fish for warm-water aquaria and also frequently used as the model animal for environmental science. However, little is known about the responses of S. aequifasciatus to low temperatures caused by environmental variation. Here, by using conventional biochemical assays and gas chromatography time-of-flight mass spectrometry metabolomics, we investigated the physiological responses of S. aequifasciatus gills exposed for 30 days to two temperature regimes: 28 °C and 20 °C. Low temperature resulted in elevated production of reactive oxygen species but not increased malondialdehyde. This might be partially related to protective responses in the antioxidant system, revealed by increased activities of superoxide dismutase and glutathione peroxidase, and level of reduced glutathione (GSH), compensating for the depletion of catalase activity. A total of 35 metabolites were identified as potential biomarkers of cold stress, showing the most influenced pathways including starch and sucrose metabolism, pentose phosphate pathway, glycerolipid metabolism, sphingolipid metabolism, glutathione metabolism, and arginine and proline metabolism. Moreover, the activation of glutathione metabolism agreed with the increased GSH level detected by biochemical assays. Overall, the results of this study suggest that low temperature can activate a protective antioxidant defence response and modify the metabolic pathways in gills of S. aequifasciatus, providing insights into the physiological regulation in response to cold stress in this tropical fish.

Microplastics have a more profound impact than elevated temperatures on the predatory performance, digestion and energy metabolism of an Amazonian cichlid.

Knowledge on the impacts of microplastics (MPs) pollution on freshwater environments and biota remains limited. Meanwhile, freshwater ecosystems have been threatened by elevated temperatures caused by climate change. To date, no information exists on how MPs-especially under elevated temperature conditions-affect predatory performance, digestive processes and metabolic pathways in freshwater organisms. Here, we examined MPs, elevated temperature and their combined effects on juveniles (0+ group) of an Amazonian cichlid, the discus fish (Symphysodon aequifasciatus). For 30 days, fish were exposed to ambient or elevated temperatures (i.e., 28 or 31 °C) in the absence or presence of MPs (i.e., 0 or 200 µg/L). The following metrics were quantified: MPs accumulation; predatory performance; and biomarkers involved in neurotransmission, digestion and energy production. The results showed that survival rate and body length were not affected by MPs, elevated temperatures or their combination. Elevated temperatures resulted in an increase in MP concentrations in fish bodies. Exposure to MPs decreased the post-exposure predatory performance (PEPP) at ambient temperatures but not at elevated temperatures. Elevated temperatures, however, had no effect on the PEPP but antagonistically interacted with MPs, leading to similar predatory performances under present and future conditions. Acetylcholinesterase (AChE) activity was only affected by MPs and decreased in the presence of MPs, indicating adverse effects in nervous and neuromuscular function and, thus, potentially in predatory performance. Trypsin activity was only influenced by MPs and decreased during exposure to MPs. Elevated temperatures or MPs alone increased the amylase activity but interacted antagonistically. Lipase activity was not influenced by either of the two stressors. In contrast, alkaline phosphatase (ALP) activity was affected by MPs or elevated temperatures alone and decreased with both stressors. Such results indicate deficits in the digestive capabilities of early-stage S. aequifasciatus under elevated temperature conditions and especially during exposure to MPs. Electron transport system (ETS) activity was not influenced by either of the two stressors. Both elevated temperatures and MPs alone increased LDH activity; however, the interaction between the two stressors cancelled activity but was still higher than activity in present conditions. Citrate synthase (CS) activity decreased with elevated temperature but increased during exposure to MPs. Cytochrome c oxidase (COX) activity was only influenced by MPs and increased in the presence of MPs. Thus, S. aequifasciatus juveniles exposed to elevated temperatures and MPs not only relied on anaerobic glycolysis for energy production but also depended on aerobic metabolism in the presence of MPs. Overall, these findings suggested that MPs showed a greater impact than elevated temperatures on the predatory performance, digestion and energy production of S. aequifasciatus. Nevertheless, juvenile survival and growth were minimally impacted, and thus, S. aequifasciatus could cope with near-future temperature increases and MP exposure.

GaAs1-xBix/GaNyAs1-y type-II quantum wells: novel strain-balanced heterostructures for GaAs-based near- and mid-infrared photonics.

The potential to extend the emission wavelength of photonic devices further into the near- and mid-infrared via pseudomorphic growth on conventional GaAs substrates is appealing for a number of communications and sensing applications. We present a new class of GaAs-based quantum well (QW) heterostructure that exploits the unusual impact of Bi and N on the GaAs band structure to produce type-II QWs having long emission wavelengths with little or no net strain relative to GaAs, while also providing control over important laser loss processes. We theoretically and experimentally demonstrate the potential of GaAs1-xBix/GaNyAs1-y type-II QWs on GaAs and show that this approach offers optical emission and absorption at wavelengths up to ~3 µm utilising strain-balanced structures, a first for GaAs-based QWs. Experimental measurements on a prototype GaAs0.967Bi0.033/GaN0.062As0.938 structure, grown via metal-organic vapour phase epitaxy, indicate good structural quality and exhibit both photoluminescence and absorption at room temperature. The measured photoluminescence peak wavelength of 1.72 µm is in good agreement with theoretical calculations and is one of the longest emission wavelengths achieved on GaAs to date using a pseudomorphically grown heterostructure. These results demonstrate the significant potential of this new class of III-V heterostructure for long-wavelength applications.

Optical gain in GaAsBi/GaAs quantum well diode lasers.

Electrically pumped GaAsBi/GaAs quantum well lasers are a promising new class of near-infrared devices where, by use of the unusual band structure properties of GaAsBi alloys, it is possible to suppress the dominant energy-consuming Auger recombination and inter-valence band absorption loss mechanisms, which greatly impact upon the device performance. Suppression of these loss mechanisms promises to lead to highly efficient, uncooled operation of telecommunications lasers, making GaAsBi system a strong candidate for the development of next-generation semiconductor lasers. In this report we present the first experimentally measured optical gain, absorption and spontaneous emission spectra for GaAsBi-based quantum well laser structures. We determine internal optical losses of 10-15 cm(-1) and a peak modal gain of 24 cm(-1), corresponding to a material gain of approximately 1500 cm(-1) at a current density of 2 kA cm(-2). To complement the experimental studies, a theoretical analysis of the spontaneous emission and optical gain spectra is presented, using a model based upon a 12-band k.p Hamiltonian for GaAsBi alloys. The results of our theoretical calculations are in excellent quantitative agreement with the experimental data, and together provide a powerful predictive capability for use in the design and optimisation of high efficiency lasers in the infrared.