Monocyte-to-High-Density Lipoprotein Cholesterol Ratio Together With the Lymphocyte-to-Monocyte Ratio in Predicting the Malignancy of the Thyroid Nodule in Patients Complicated With Type 2 Diabetes.

Background: Thyroid nodules, although mostly benign and symptomless, have a small chance of being cancerous, necessitating accurate diagnosis. This study aims to develop and validate a nomogram for differentiating malignant and non-malignant thyroid nodules in individuals with type 2 diabetes. Methods: The study included 484 patients with both thyroid nodules and type 2 diabetes who underwent thyroid gland lobectomy at Wenzhou Medical University Hospital. Optimal cutoff values for continuous variables were determined using ROC curve analysis. Significant factors identified in univariable analysis were used to construct the nomogram. The monocyte-to-high-density lipoprotein cholesterol ratio (MHR) was visualized through a histogram and scatter diagram. Discriminatory power was assessed using ROC analysis, and calibration curves ensured consistency. Decision curve analysis (DCA) evaluated clinical benefits. Results: The cohort was divided into a training group (70%) and an internal validation group (30%). The scatter diagram revealed a correlation between MHR levels and the proportion of goiter cases, with higher MHR levels associated with increased goiter incidence. The histogram showed higher average MHR levels in goiter patients compared to those with papillary thyroid carcinoma (PTC) in both groups. Multivariate logistic regression identified age, total cholesterol (TC), triglyceride (TG), fasting blood sugar (FSG), fibrinogen, lymphocyte-to-monocyte ratio (LMR), and MHR as independent predictive factors for malignancy in thyroid nodules with type 2 diabetes. The nomogram achieved high discrimination, with C-index values of 0.901 (training data set) and 0.760 (internal validation data set). Calibration curves displayed good agreement, and DCA demonstrated significant net clinical benefits. Conclusions: MHR is associated with sex, serum cholesterol levels, and peripheral blood cell counts, making it a potential novel biomarker for differentiating between PTC and goiter in type 2 diabetes patients.

Expression and significance of MMP-9 and MDM2 in the oncogenesis of lung cancer in rats.

OBJECTIVE: To observe the expression of matrix metalloproteinase-9 (MMP-9) and mouse double minute 2 homolog (MDM2) in the oncogenesis of lung cancer in rats and to explore their clinical value. METHODS: A total of 140 rats were selected, of which 20 were selected randomly as the control group; and the remaining 120 as the observation group. The observation group was injected with benzopyrene to establish diseases model such as tissue proliferation, abnormal proliferation and lung cancer. Detected the MMP-9 levels of lung tissue by enzyme-linked assay, detected the MDM2 levels of lung tissue by immunochemistry assay. RESULTS: The MMP-9 and MDM2 expression of the lung cancer group and the abnormal proliferation group were significantly higher than that in the tissue proliferation group and the control group, the difference was significant (P<0.05). And the MDM2 expression of the tissue proliferation group was significantly higher than that in the control group, the difference was significant (P<0.05). There was no significant difference in the MMP-9 expression between the tissue proliferation group and the control group (P>0.05). The MDM2 and MMP-9 expression were increased in turn in the small cell carcinoma, squamous cell carcinoma and adenocarcinoma, the difference was statistically significant (P<0.05). The MMP-9 and MDM2 expressions of stage III and stage IV lung cancer tissue in rats were significant higher than that during stage I and stage II, the difference was significant (P<0.05). There was no significantly different in the MMP-9 and MDM2 expressions between stage III and stage IV(P>0.05), and there is no significant difference of the MMP-9 and MDM2 expressions between stage I and stage II(P>0.05). CONCLUSIONS: The expression of MMP-9 and MDM2 in lung tissue was associated with lung disease and lung cancer, both of them may be involved in the development and metastasis of lung cancer. Combined detection can be used as therapy and prognostic indicators for lung cancer.

Expression and significance of MMP-9 and MDM2 in the oncogenesis of lung cancer in rats

OBJECTIVE@#To observe the expression of matrix metalloproteinase-9 (MMP-9) and mouse double minute 2 homolog (MDM2) in the oncogenesis of lung cancer in rats and to explore their clinical value.@*METHODS@#A total of 140 rats were selected, of which 20 were selected randomly as the control group; and the remaining 120 as the observation group. The observation group was injected with benzopyrene to establish diseases model such as tissue proliferation, abnormal proliferation and lung cancer. Detected the MMP-9 levels of lung tissue by enzyme-linked assay, detected the MDM2 levels of lung tissue by immunochemistry assay.@*RESULTS@#The MMP-9 and MDM2 expression of the lung cancer group and the abnormal proliferation group were significantly higher than that in the tissue proliferation group and the control group, the difference was significant (P0.05). The MDM2 and MMP-9 expression were increased in turn in the small cell carcinoma, squamous cell carcinoma and adenocarcinoma, the difference was statistically significant (P0.05), and there is no significant difference of the MMP-9 and MDM2 expressions between stage I and stage II(P>0.05).@*CONCLUSIONS@#The expression of MMP-9 and MDM2 in lung tissue was associated with lung disease and lung cancer, both of them may be involved in the development and metastasis of lung cancer. Combined detection can be used as therapy and prognostic indicators for lung cancer.