ABSTRACT
OBJECTIVE: To investigate the HPLC-ELSD fingerprints of Reineckia carnea from different habitats and provide a theoretical evidence to evaluate its quality. METHODS: The chromatographic conditions were Diamonsil (R) C18 column (4.6 mm x 250 mm, 5 microm) by linear gradient elution using water and acetonitrile as mobile phase at a flow rate of 0.8 mL/min, column temperature at 25 degrees C and sample injection 30 microL. An Agilent 1260 infintiy ELSD operated at 85 degrees C, gain 6 and 3.5 bar nitrogen. RESULTS: The HPLC-ELSD fingerprints were established with 6 common peaks and methodology reached the required standard. CONCLUSION: The method is simple, rapid, accurate, and can be used for quality evaluation of Reineckia carnea from different habituts.
