ABSTRACT
The patient presented to the clinic with painful muscle swelling in the right lower extremity, which improved with immunosuppressive therapy. Initially, the condition was diagnosed as polymyositis but recurred soon after. After imaging and biopsy, the final diagnosis was primary skeletal muscle peripheral T-cell lymphoma, not otherwise specified (PSM-PTCL, NOS). In this report, we discuss the challenges in diagnosing and treating this aggressive malignancy and review the literature on PSM-PTCL, NOS. Key Points ⢠To date, there are few reports of PSM-PTCL, NOS, and our case is the tenth. ⢠It is crucial to consider PSM-PTCL, NOS, when presenting with localized muscle edema and unexplained pain. ⢠Histopathological examination is likely the most effective method for diagnosing this rare disease.
Subject(s)
Lymphoma, T-Cell, Peripheral , Muscle, Skeletal , Myositis , Humans , Lymphoma, T-Cell, Peripheral/complications , Lymphoma, T-Cell, Peripheral/pathology , Lymphoma, T-Cell, Peripheral/diagnosis , Myositis/diagnosis , Myositis/complications , Myositis/pathology , Muscle, Skeletal/pathology , Male , Muscle Neoplasms/diagnostic imaging , Middle Aged , Biopsy , Immunosuppressive Agents/therapeutic use , Magnetic Resonance ImagingABSTRACT
OBJECTIVE: Identification of reliable biomarkers for the diagnosis and prognosis of gastric cancer (GC) is very important for achieving early cancer detection and improving clinical outcomes. The aim of the present study was to explore the clinical significance of serum exosomal miR-134 in GC. METHODS: A total of 133 GC cases were enrolled in this study, and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out to measure the relative serum exosomal miR-134 expression level. The association between serum exosomal miR-134 expression and clinicopathological characteristics was investigated. RESULTS: Our results showed that serum exosomal miR-134 expression was significantly lower in GC patients than in controls. In addition, serum exosomal miR-134 discriminated GC cases from healthy controls with high accuracy. Moreover, reduced serum exosomal miR-134 expression was closely associated with aggressive clinical variables including TNM stage, lymph node metastasis and invasion depth. Furthermore, Kaplan-Meier analysis revealed that GC patients with low serum exosomal miR-134 expression tended to have shorter overall survival and relapse free survival. In multivariate analysis, serum exosomal miR-134 was an independent prognostic marker for GC. CONCLUSION: In conclusion, serum exosomal miR-134 might serve as a promising diagnostic and prognostic biomarker for GC.
Subject(s)
MicroRNAs , Stomach Neoplasms , Biomarkers, Tumor/genetics , Humans , MicroRNAs/metabolism , Neoplasm Recurrence, Local/pathology , Neoplasm Staging , Prognosis , Stomach Neoplasms/diagnosis , Stomach Neoplasms/geneticsABSTRACT
BACKGROUND: In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility. RESULTS: We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes. CONCLUSIONS: Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity.
Subject(s)
Adaptation, Physiological , Droughts , Oryza/enzymology , Oryza/physiology , Protein Kinases/metabolism , Stress, Physiological , Abscisic Acid/pharmacology , Adaptation, Physiological/drug effects , Adaptation, Physiological/genetics , Dehydration , Fertility/drug effects , Gene Expression Regulation, Plant/drug effects , Organ Specificity/drug effects , Organ Specificity/genetics , Oryza/anatomy & histology , Oryza/genetics , Osmosis/drug effects , Plant Stomata/drug effects , Plant Stomata/physiology , Plants, Genetically Modified , Pollen/drug effects , Pollen/growth & development , Proline/metabolism , RNA Interference/drug effects , Solubility , Stress, Physiological/drug effects , Stress, Physiological/geneticsABSTRACT
Esophageal cancer is a common malignancy, for which surgery is the most effective treatment. Compared with traditional surgery, video-assisted thoracoscopic and laparoscopy minimally invasive surgery enables less trauma, better visibility, reduced bleeding and postoperative pain, and lower incidence of surgical complications through a minimally invasive, safe, and highly cost-effective approach in favor of early rehabilitation after surgery. Therefore, the promotion and application of this surgical approach will undoubtedly benefit the majority of patients with esophageal cancer. We have performed video-assisted thoracoscopic and laparoscopy minimally invasive surgery for more than 150 patients in our hospital to date, and have carried out a series of studies in this regard. As the video shows, this approach is safe and reliable with minimal injury and bleeding.
