ABSTRACT
BACKGROUND/AIM: To determine if long-chain non-coding RNA (lncRNA) MIR4435-2HG (MIR4435) expression is associated with pre-malignant colon polyps and colon cancer. MATERIALS AND METHODS: Children's colonic-polyp specimens were sequenced for MIR4435 expression. LncRNA MIR4435 expression data in colorectal cancer and normal intestinal tissues were retrieved from The Cancer Genome Atlas (TCGA). The proliferation, adhesion, and invasion ability of human colon-cancer cell line HCT116 with or without MIR4435 knockdown was analyzed. The expression of Smad4, desmoplakin, and ß-catenin genes was detected by western blotting in HCT116 cells. RESULTS: MIR4435 expression correlated with the size of intestinal polyps in children. Expression of MIR4435 was up-regulated in colorectal cancer. MIR4435 knockdown in HCT116 cells inhibited their proliferation, adhesion, and invasion ability. Smad4 and desmoplakin were up-regulated and ß-catenin was down-regulated in HCT116 cells by MIR4435 knockdown. CONCLUSION: MIR4435 expression correlated with the size of intestinal polyps in children and with the proliferation, adhesion, and invasion ability of colon-cancer cells and was upregulated in colon cancer.
Subject(s)
Colonic Neoplasms , Intestinal Polyps , RNA, Long Noncoding , Cell Line, Tumor , Cell Proliferation/genetics , Child , Colonic Neoplasms/genetics , Desmoplakins/genetics , Desmoplakins/metabolism , Gene Expression Regulation, Neoplastic , Humans , Intestinal Polyps/genetics , Neoplasm Metastasis , RNA, Long Noncoding/genetics , beta Catenin/genetics , beta Catenin/metabolismABSTRACT
Detoxification plays an important role in herbicide action. Herbicide safeners selectively protect crops from herbicide injury without reducing the herbicidal efficiency against the target weeds. With the large-scale use of herbicides, herbicide safeners have been widely used in sorghum, wheat, rice, corn, and other crops. In recent years, an increasing number of unexpected new herbicide safeners have been designed. The varieties, structural characteristics, uses, and synthetic routes of commercial herbicide safeners are reviewed in this paper. The design ideas and structural characteristics of novel herbicide safeners are summarized, which provide a basis for the design of bioactive molecules as new herbicide safeners in the future.
Subject(s)
Herbicides , Herbicides/chemistry , Herbicides/pharmacology , Plant Weeds , Triticum , Zea mays/chemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of berberine on expressions of tumor necrosis factor alpha (TNF-alpha) and receptor type I (TNFR1) in Abeta25-35-induced inflammatory reaction in SH-SYSY cell lines.</p><p><b>METHOD</b>The 5 micromol . L-1 Abeta25-35 was used to treat SH-SY5Y cells for 24 hours, in order to establish the Alzheimer's disease (AD) model. Before modeling, berberine was given for pretreatment for 2 hours. The experiment included the normal control group, the AD model group, and indometacin low dose and high dose groups. Spectrophotometry was adopted to detect the activity of LDH. Meanwhile, the level of TNF-alpha was determined by ELISA, and the expression of TNFR1 genes was detected by RT-PCR.</p><p><b>RESULT</b>Compared with the normal control group, the AD cell model group showed significant increase in LDH, TNF-alpha, and TNFR1 gene and protein expressions in the culture media. After intervention with berberine, the activity of LDH and TNF-alpha reduced in cell supernatant. The intervention with berberine could down-regulate TNFR1 gene and protein expressions, particularly 1, 10 x 10(-6) mol . L-l berberine showed a more notable effect in regulating TNFR1.</p><p><b>CONCLUSION</b>Berberine has the protective effect in Abeta-induced inflammatory injury in SH-SY5Y cells. Its mechanism may be related to the expression of its anti inflammatory factor TNF-alpha and its type I receptor TNFR1. Specifically, its regulation to TNFR1 shows dose dependence.</p>
