ABSTRACT
OBJECTIVE: To optimize the technology of enzymolysis extraction of total anthraquinones. METHODS: Used the total anthraquinone concentration extracted from rhubarb (Rheum palmatum, L) via enzymolysis as experimental assess index, effect of enzyme dose, enzyme solution temperature, enzyme solution time and pH on total anthraquinone extraction were investigated respectively based on single factor variance analysis. Furthermore, its extraction process was optimized based on response surface methodology. RESULTS: The yield of total anthraquinones demonstrated the top (3.1730 +/- 0.1023 mg/L) while the enzyme dose was up to 0.12 g, besides, for the enzyme solution time, the total anthraquinones yield exhibited a trend of increase with the increase of enzyme solution time and the top concentration of total anthraquinones was up to 2.1410 +/- 0.1252 mg/L while it was 48 h; Moreover, the top field of total anthraquinones were up to 2.2777 +/- 0.2133 mg/L and 4.1360 +/- 0.3877 mg/L while the pH and enzyme solution temperature were up to 4.8 and 50 degrees C in respectively. Finally, the most optimum enzymolysis extraction processing technology via response surface methodology was as follows: pH: 4.59, enzyme dose: 0.1 g as well as enzyme solution temperature: 45.86 degrees C. CONCLUSION: The condition of extraction processing technology is easily-controlled, simple and lower cost, and the final extraction concentration of total anthraquinone is up to 4.3231 mg/L under the processing technology above.
