Simplicillium sinense sp. nov., a novel potential pathogen of tinea faciei.

Simplicillium species are widely distributed with a broad spectrum of hosts and substrates. Generally, these species are entomopathogenic or mycoparasitic. Notably, some isolates of Simplicillium lanosoniveum and Simplicillium obclavatum were obtained from human tissues. In this study, two fungi were isolated from the annular itchy patch of infected skin of a 46-year-old man with diabetes mellitus. Based on a combination of morphological characteristics and phylogenetic analysis, a novel species, Simplicillium sinense, was introduced herein. It morphologically differs from the remaining Simplicillium in the size of phialides and conidia. Additionally, it grows slowly on YPD at 37°C. Antimicrobial susceptibility testing presented that this fungus is resistant to most azole antifungals. Therefore, the diagnosis of tinea faciei was made, and after 2 weeks of being treated with oral terbinafine (250 mg, once a day) and topical terbinafine cream for 1 month, the rash was mainly resolved and no recurrence happened after 6 months of follow-up. Herein, Simplicillium sinense was introduced as a new fungal taxon. Meanwhile, a case of superficial infection caused by S. sinense was reported. So far, it is the third Simplicillium species obtained from human tissue. Meanwhile, terbinafine is recommended as the first-line antifungal treatment against Simplicillium infection.

Four new species of Trichoderma in the Harzianum clade from northern China.

The Harzianum clade of Trichoderma comprises many species, which are associated with a wide variety of substrates. In this study, four new species of Trichoderma, namely T. lentinulae, T. vermifimicola, T. xixiacum, and T. zelobreve, were encountered from a fruiting body and compost of Lentinula, soil, and vermicompost. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the gene encoding the second largest nuclear RNA polymerase subunit (RPB2), the translation elongation factor 1-α encoding gene (TEF1-α). The analysis combining sequences of the three gene regions distinguished four new species in the Harzianum clade of Trichoderma. Among them, T. lentinulae and T. xixiacum clustered with T. lixii, from which these new species differ in having shorter phialides and smaller conidia. Additionally, T. lentinulae differs from T. xixiacum in forming phialides with inequilateral to a strongly-curved apex, cultural characteristics, and slow growth on PDA. Trichoderma vermifimicola is closely related to T. simmonsii, but it differs from the latter by producing phialides in verticillate whorls and smaller conidia. Trichoderma zelobreve is the sister species of T. breve but is distinguished from T. breve by producing shorter and narrower phialides, smaller conidia, and by forming concentric zones on agar plates. This study updates our knowledge of species diversity of Trichoderma.

Changes of amplitude integrated electroencephalogram during brain development in premature / 中华实用儿科临床杂志

Objective To explore the changes of amplitude integrated electroencephalogram (aEEG) during brain development in premature.Methods One hundred and forty-eight premature (gestational age 28-36 weeks) were selected.According to their gestational ages,148 premature were divided into 28-30 weeks group(40 cases),with 21 male,19 female;＞30-33 weeks group (49 cases),with 25 male,24 female;＞33-36 weeks group (59 cases),33 male,26 female.At the same time,40 cases of gestational age 37-40 weeks full-term newborns were selected as control group,with 23 male,17 female.Olympic CFM 6000 cerebral function monitor(produced by the American Natus company) was used to detect the changes of their aEEG,and analysis of the changes of aEEG were conducted.The first monitor was performed 3 days after birth,then once a week,monitoring for 2 hours each time,till the premature were discharged from hospital.The average of the monitoring data was obtained as a result for the analysis,and data analysis was conducted by using SAS 9.0 statistical software.Results The sleep-wake cycle continuity voltage positive rate and cycle occurrence rate of the 28-30 weeks,＞ 30-33 weeks,＞ 33-36 weeks premature were significantly lower than those of the 37-40 weeks full-term newborns (all P ＜ 0.05).The 28-30 weeks,＞ 30-33 weeks,＞ 33-36 weeks premature spectrun lower bound voltage value of quiet sleep cycle and activity sleep cycle were significantly lower than those of the 37-40 weeks full-term newborns (all P ＜ 0.05).The 28-30 weeks,＞ 30-33 weeks,＞ 33-36 weeks premature spectrum width of quiet sleep cycle and activity sleep cycle were significantly wider than the 37-40 weeks full-term newborns (F =13.444,11.982,all P ＜ 0.05).The 28-30 weeks,＞ 30-33 weeks,＞ 33-36 weeks premature total duration of sleep cycle were significantly higher than the 37-40 weeks full-term newborns (F =12.451,P ＜ 0.05).Conclusions With the increase of gestational age,premature brain development becomes gradually mature,and aEEG can reflect the degree of premature brain development to some extent.

Features of detection of facial expressions in college students with mobile phone dependence / 中国心理卫生杂志

Objective:To investigate the mobile phone dependence college students'features of detection of different facial expressions and give suggestion to future intervention for mobile phone dependence students.Methods:Totally 548 college students were assessed with the Mobile Phone Addiction Tendency Scale (MPATS).Two groups were screened by cluster analysis and high and low group standard,namely,mobile phone dependence group and control group,40 participants in each.A visual search task was used to examine the efficiency of facial processing.Participants searched displays of schematic faces and were required to determine whether the faces displayed were all the same or whether one was different.Results:When the expressions of the faces were the same,the main effect of number of faces was significant [F(1,468) =11.26,P ＜ 0.01],the detection of four faces was faster than that of eight faces [(1079.1 ± 187.9) msvs.(1139.2 ±202.7) ms].When the expressions of the faces were different,the main effect of expression types was statistically significant [F (1,312) =10.30,P ＜ 0.01],the detection of sad faces was faster than that of happy faces [(941.0 ± 168.5) ms vs.(997.8 ± 152.7) ms,P ＜ 0.01].The types of expressions had significant interaction with groups of participants [F (1,312) =16.55,P ＜ 0.01],mobile phone dependence group detected the sad faces significantly faster than the control group [(975.5 ± 112.4) ms vs.(906.4 ± 126.5) ms,P ＜0.05].Conclusion:The detection of sad faces is faster in those with mobile phone dependence than in controls.The detection of the sad and happy faces are serial search in both groups.

[Study on molecular recognition technology in active constituents extracted and isolated from Aconitum pendulum].

OBJECTIVE: To investigate CD molecular recognition technology applied in active constituents extracted and isolated from traditional Chinese medicine--Aconitum pendulum. METHODS: The inclusion constant and form probability of the inclusion complex of Aconitum pendulum with p-CD was calculated by UV spectra method. The active constituents of Aconitum pendulum were extracted and isolated by molecular recognition technology. The inclusion complex was identified by UV. The chemical constituents of Aconitum pendulum and inclusion complex was determined by HPLC. The analgesic effects of inclusion complex was investigated by experiment of intraperitoneal injection of acetic acid in rats. RESULTS: The inclusion complex was identified and confirmed by UV spectra method, the chemical components of inclusion complex were simple, and the content of active constituents increased significantly, the analgesic effects of inclusion complex was well. CONCLUSION: The molecular recognition technology can be used for extracting and isolating active constituents of Aconitum pendulum, and the effects are obvious.

Role strain of different gender nursing students in obstetrics practice: a comparative study.

The purpose of this study was to compare gender-based differences in levels of role strain and related attitudes among nursing students and to examine factors associated with role strain experienced by students during obstetric practice. A cross-sectional survey was used on a sample of 95 female and 55 male baccalaureate nursing students who had completed their obstetric practice at one of two universities in central Taiwan. Results showed role strain in male nursing students to be significantly higher than that in female nursing students, especially in terms of role incongruity. The attitude of male nursing students toward clinical instructors, healthcare providers, and clients and their families and the stereotyped viewpoint about the gender role and occupation were all significantly higher than those of their female counterparts. Most variance (57.3%) in role strain in male nursing students was explained by attitude toward clinical instructors, attitude toward clients with their families, a lack of interest in nursing, and attitude toward healthcare providers. By way of comparison, attitude toward clinical instructors, attitude toward clients and their families, and attitude toward healthcare providers explained 30.1% of the variance of role strain in female nursing students. Results of this study may enhance understanding of the experiences of nursing students in obstetric practice and serve as a reference for nursing educators on curriculum development and clinical instruction.

[Value of MR imaging and MR angiography in the differential diagnosis of carotid space tumour].

OBJECTIVE: To determine the imaging features of magnetic resonance imaging (MRI) and 2D time of flight (TOF) MR angiography (MRA) and study the value in the differential diagnosis and surgical planning for carotid space tumors. METHODS: Twenty-six patients with suspected pulsatile carotid space mass were imaged by MRI and 2D TOF MRA from 1996 to 2003. Its characteristic findings were analyzed for lesion shape, margin, signal intensity, angle of common carotid bifurcation, and the relationship between the great vessels and carotid space mass. RESULTS: Of the 26 patients, 22 were verified histopathologically, including 15 carotid body tumors (1 patient had bilateral carotid body tumors), 4 carotid artery aneurysms, 3 schwannomas, and 1 metastatic carcinoma. The rest four patients had clinical pseudomasses proved by MRI and MRA as considerable dilated or tortuous carotid artery as compared with the contralateral one. Combined MRI and MRA assessment of carotid body tumors and carotid artery aneurysm yielded an accuracy of 100%. It was also revealed that the anatomy shown on the MRI and axial MRA source images was consistent with that found by surgery. CONCLUSION: MRI in combination with MRA is considered as non-invasive imaging technique for the evaluation of carotid space tumor showing superiority to other modalities in the differential diagnosis between vascular versus non-vascular tumours. This method may take the place of traumatic carotid angiography.

Value of MR imaging and MR angiography in the differential diagnosis of carotid space tumour / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To determine the imaging features of magnetic resonance imaging (MRI) and 2D time of flight (TOF) MR angiography (MRA) and study the value in the differential diagnosis and surgical planning for carotid space tumors.</p><p><b>METHODS</b>Twenty-six patients with suspected pulsatile carotid space mass were imaged by MRI and 2D TOF MRA from 1996 to 2003. Its characteristic findings were analyzed for lesion shape, margin, signal intensity, angle of common carotid bifurcation, and the relationship between the great vessels and carotid space mass.</p><p><b>RESULTS</b>Of the 26 patients, 22 were verified histopathologically, including 15 carotid body tumors (1 patient had bilateral carotid body tumors), 4 carotid artery aneurysms, 3 schwannomas, and 1 metastatic carcinoma. The rest four patients had clinical pseudomasses proved by MRI and MRA as considerable dilated or tortuous carotid artery as compared with the contralateral one. Combined MRI and MRA assessment of carotid body tumors and carotid artery aneurysm yielded an accuracy of 100%. It was also revealed that the anatomy shown on the MRI and axial MRA source images was consistent with that found by surgery.</p><p><b>CONCLUSION</b>MRI in combination with MRA is considered as non-invasive imaging technique for the evaluation of carotid space tumor showing superiority to other modalities in the differential diagnosis between vascular versus non-vascular tumours. This method may take the place of traumatic carotid angiography.</p>