ABSTRACT
Objective To explore the association between inducible nitric oxide synthase (iNOS) gene polymorphisms and ischemic stroke in Uygur population of Xinjiang.Methods A case-control study was performed,included 316 patients with ischemic stroke,admitted to our hospital from June 2007 to December 2013,and 316 age-and gender-matched healthy subjects in Uygur population of Hami.The genotypes ofrs2779248(C/T) and rs1137933(C/T) were determined by Taqman probe fluorescent quantitative PCR.Results The distribution frequencies of rs2779248 (CC,CT and TT) were 51.9%,42.1% and 6.0% in the patient group,ands 61.7%,30.1% and 8.2% in the controls,respectively.As compared with those with rs2779248 CC genotype,individuals with CT+TT genotype and CT genotype had higher risk ofischemic stroke,the odds ratio was 1.49 (95%CI:1.09-2.05,P=0.013)and 1.66 (95%CI:1.19-2.32,P=0.003).The distribution frequencies ofrs1137933 (CC,CT and TT) were 66.8%,28.5% and 4.7% in the patient group,ands 66.8%,27.8% and 5.4% in the controls,respectively;no significant difference was noted between the patient group and controls in the distribution frequencies (P>0.05).Multi-factor Logistic regression analysis indicated that mutation of genotype C>T in rs2779248was the independent risk factor of ischemic stroke in Uygur population of Hami after adjusting related risk factors (OR=1.55,95%CI:1.00-2.40,P=0.049).Conclusion The polymorphisms ofrs2779248 in iNOS gene may modify the risk of ischemic stroke in Uygur population of Hami,but no association is found in rs1137933.
ABSTRACT
Objective To investigate the correlation between inducible nitric oxide synthase (iNOS) gene polymorphism and ischemic stroke in Chinese Han population.Methods Patients with first-ever stroke and the age-and sex-matched healthy controls were enrolled in the study.Taqman probe fluorescence quantitative polymerase chain reaction technique was used to detect the genotype distribution of rs2779248 C/T and rs1137933 C/T polymorphisms.Results A total of 246 patients with ischemic stroke and 246 controls were enrolled.The distribution frequencies of rs2779248 CC,CT and TT genotypes in the patient group were 57.7%,36.6%,and 5.7%,respectively,and in the control group were 68.7%,28.0%,and 3.3%,respectively.The T allele frequency of the patient group was significantly higher than that of the control group (24.0% vs.17.3%;P =0.015).Multivariate logistic regression analysis showed that after adjusting for the risk factors including age,sex,hypertension,and diabetes,the risk of ischemic stroke in the CT + TT genotype carriers was 1.64 times of the CC genotype carriers (odds ratio 1.64,95% confidence interval 1.07-2.51;P=0.022).The distribution frequencies of rs1137933 CC,CT and TT in the patient group were 58.1%,37.8%,and 4.1%,respectively and in the control group were 68.3%,29.3%,and 2.4%,respectively.The T allele frequency of the patient group was significantly higher than that of the control group (23.0% vs.17.1%;P =0.013).Multivariate logistic regression analysis showed that after adjusting for traditional risk factors,the risk of ischemic stroke of patients with the TT + CT genotype carriers was 1.60 times of the CC genotype carriers (odds ratio 1.60,95% confidence interval 1.05-2.46;P=0.030).Conclusions The rs2779248 C/T and rs1137933 C/T polymorphisms in iNOS gene may be associated with the risk of onset of ischemic stroke in Chinese Han population.