ABSTRACT
To illustrate the relationship between environment hydrophobicity and soybean peptide and its calcium complexes when they are absorbed transmembrane, different solution environments (HBS buffer, TFE hydrophobic solution and cell suspension) were used to simulate hydrophilic and hydrophobic environments. In this study, soybean peptides (10-30 kDa) with a high calcium binding capacity were prepared by enzymatic hydrolysis and ultrafiltration. The results of cell experiments showed that the peptide could transport calcium into cells for absorption. Secondary structure changes of the peptide and its calcium complexes in different solution environments showed that the secondary structure of the peptide changed during the transmembrane absorption, and the contents of α-helix and ß-sheet structures increased. Besides, the ß-sheet structures in the peptide-calcium complexes were further converted to an α-helix structure. This conversion may be induced by the hydrophobicity of peptide solutions. In addition, when the conformation changes, the positively charged peptides in the sample will be exposed and then interact with cells, which is beneficial for the transmembrane of peptide-calcium complexes.
Subject(s)
Calcium/chemistry , Glycine max/chemistry , Peptides/chemistry , Buffers , Circular Dichroism , Hydrolysis , Hydrophobic and Hydrophilic Interactions , Phosphates/chemistry , Protein Conformation , Protein Conformation, alpha-Helical , Protein Structure, Secondary , Trifluoroethanol/chemistryABSTRACT
[ ABSTRACT] AIM:To investigate the effect of Jia-jian-yi-yin decoction on endothelium-dysfunction in ovariecto-mized rats.METHODS:The ovariectomized rats were treated with Jia-jian-yi-yin decoction or turbid liquid of estradiol va-lerate for 8 weeks.The vascular ring tension was measured.Scanning electron microscopy and Western blotting were ap-plied to assess the function of endothelium-dependent dilation, aortic endothelial morphology and the expression of endothe-lial lipase.The pathologic sections were prepared to observe the effect of Jia-jian-yi-yin decoction on the uterus.RE-SULTS:In ovariectomized rats, the decrease in endothelium-dependent relaxation to acetylcholine ( ACh) was reversed to normal level, the endothelial morphology returned to normal without lipid accumulation and the endothelial lipase expression was decreased by Jia-jian-yi-yin decoction.Furthermore, no obvious change of the wet weight of uterine between the ovari-ectomized rats with or without Jia-jian-yi-yin decoction treatment was observed.CONCLUSION:Jia-jian-yi-yin decoction may have protective effects on endothelium-dependent vasodilation and aortic endothelial morphology in estrogen-deficient animals.
ABSTRACT
ObjectiveTo evaluate the efficacy of preoperative transarterial chemo-embolization and radical surgery for renal carcinoma.MethodsA lotal of 68 cases underwent transarterial chem-embolization by injecting lipiodcl-MMC emulsion,DDP,ADM and gelain sponge successfully and 64 of them underwent radical surgery 82～110 h after embolization.The efficacy was retrospectively studied.ResultsPreoperative transarterial chem-embolization made resection easier,with 1,3,5,and 8 year survival rate being 91.7%,83.3%,66.7% and 45.0%,respectively.The side effect of chemo-embolization were lumbago in all,fever in 52,gastrointestive reaction in 48.No extra-renal embolization has been noted.ConclusionsPreoperative transarterial chem-embolization is helpful and is highly recommented as a preoperative routine procedure.It might serve as one of the main procedures for non-operatabe cases.
