Emotional and behavioral effects of after-school tutoring on school-age children in a district in Shanghai / 上海预防医学

ObjectiveTo explore the effects of after-school tutoring for school-age children on their emotions and behaviors， and to provide a basis for developing after-school tutoring and psychological support strategies for school-age children. MethodsFour elementary schools in a district of Shanghai were selected by simple random sampling method. Whole group sampling was conducted by class， and parents of students were surveyed by general questionnaire and the scale of strengths and difficulties. Information was collected on the basic conditions of children and families and the results were compared and analyzed. ResultsThis study showed that 88.26% of children attended extracurricular classes， of which 26.16% attended 3 or more classes， 42.5% attended cultural classes， 28.3% attended sports classes， and 59.8% attended art classes. Children who did not attend classes had higher pro-social scores. Children who attended reading class had lower scores for emotional problems that was a protective factor for emotional problems. Children who chose extracurricular programs in sports and reading also had lower overall scores for difficulties. Excessive use of electronic devices by school-age children on weekdays or weekends had a negative effect on emotional and conduct problem scores regardless of whether they chose extracurricular programs. It appeared that the choice of cultural programs， art programs and different levels of exercise intensity had no effect on children's emotions and behaviors. ConclusionFor children with low pro-social level， extracurricular classes should be carefully selected. For extracurricular classes， more reading and sports-related courses can be considered， which have positive effects on school-age children’s emotions and behaviors. Regardless of the choice of extracurricular classes， try to avoid excessive online classes.

A latent class analysis of feeding practices among preschoolers’ parents and its correlations with parental depression status / 中华行为医学与脑科学杂志

Objective:To explore the latent classes of parental feeding behaviors in preschool children, and to examine the relationship between potential types of parental feeding behaviors and parental depression status.Methods:From May to July 2021, parents of preschoolers from eight kindergartens in Shanghai were recruited and investigated.A paper-based questionnaire consisting of the Chinese preschooler’s caregivers’ feeding behavior scale (CPCFBS) and the center for epidemiological studies depression scale (CESD-10) was employed.Finally, a total of 1 006 valid questionnaires were retrieved.Mplus 8.0 and SPSS 26.0 were used for statistical analysis.The latent class analysis was used to identify subgroups of parents based on their feeding practices.Multinomial Logistic regression was used to examine the relevant influencing factors.Results:The feeding behaviors of parental of preschool children could be divided into four potential categories: " high responsiveness and high non-responsiveness type" (24.55%), " low responsiveness and high non-responsiveness type" (27.44%), " high responsiveness and low non-responsiveness type" (28.33%) and " low responsiveness and low non-responsiveness type" (19.68%). The parents with depression status were less likely to be categorized as " high responsiveness and low non-responsiveness type" ( OR=0.386, 95% CI: 0.218-0.684). The parents with older children were more likely to be categorized as " low responsiveness and low non-responsiveness type" ( OR=1.318, 95% CI: 1.039-1.672). Conclusion:The feeding practices of parents of preschool children can be categorized into four latent classes.The parents with depression status are more likely to adopt non-responsive feeding practice than responsive feeding practice.Actively paying attention to and improving the depression status of parents may help the feeder to adopt scientific feeding behavior.

Analysis on the status and influencing factors of health care intention in transitional children and adolescents with diabetes / 中国实用护理杂志

Objective:To investigate the intention to participate in health care in transitional children and adolescents with diabetes and analyze its influencing factors.Methods:This was a cross-sectional survey study. From March to October 2021, 185 children and adolescents with diabetes were selected as research subjects by convenience sampling method from People′s Hospital Affiliated to Jiangsu University. General data questionnaire, Participation in Health Care Intention Questionnaire, Health Literacy Scale and Diabetes Self-management Scale were used to investigate, and multiple linear regression was used to analyze the influencing factors of the population′s intention to participate in health care.Results:The transitional children and adolescents with diabetes participation in health care intention total score was (124.87 ± 16.31) points, the health literacy total score was (33.70 ± 4.38) points, diabetes self-management total score was (35.11 ± 5.19) points. The regression analysis found that age, course of the disease, diabetes type, family structure modes, health literacy, and self-management ability were the main factors influencing adolescents involved in health care intention ( t values were -1.99-2.66, all P<0.05), including health literacy ability and disease management ability was positively correlated with the disease ( r = 0.250, 0.232, both P<0.01). Conclusions:The transitional children and adolenscents with diabetes have a medium level of intention to participate in health care. The transitional children and adolenscents with older age, longer disease course and nuclear family structure had higher levels of health care intention. The higher the level of health literacy and self-management, the higher the level of intention to participate in health care, medical staff should take targeted measures to improve the intention to participate in health care, promote this group to improve disease management ability and quality of life.

A pan-variant mRNA-LNP T cell vaccine protects HLA transgenic mice from mortality after infection with SARS-CoV-2 Beta

Clinically licensed COVID-19 vaccines ameliorate viral infection by inducing vaccinee production of neutralizing antibodies that bind to the SARS-CoV-2 Spike protein to inhibit viral cellular entry (Walsh et al., 2020; Baden et al., 2021), however the clinical effectiveness of these vaccines is transitory as viral variants arise that escape antibody neutralization (Tregoning et al., 2021; Willett et al., 2022). Vaccines that solely rely upon a T cell response to combat viral infection could be transformational because they can be based on highly conserved short peptide epitopes that hold the potential for pan-variant immunity, but a mRNA-LNP T cell vaccine has not been shown to be sufficient for effective antiviral prophylaxis. Here we show that a mRNA-LNP vaccine based on highly conserved short peptide epitopes activates a CD8+ and CD4+ T cell response that prevents mortality in HLA-A*02:01 transgenic mice infected with the SARS-CoV-2 Beta variant of concern (B.1.351). In mice vaccinated with the T cell vaccine, 24% of the nucleated cells in lung were CD8+ T cells on day 7 post infection. This was 5.5 times more CD8+ T cell infiltration of the lungs in response to infection compared to the Pfizer-BioNTech Comirnaty(R) vaccine. Between days 2 and 7 post infection, the number of CD8+ T cells in the lung increased in mice vaccinated with the T cell vaccine and decreased in mice vaccinated with Comirnaty(R). The T cell vaccine did not produce neutralizing antibodies, and thus our results demonstrate that SARS-CoV-2 viral infection can be controlled by a T cell response alone. Our results suggest that further study is merited for pan-variant T cell vaccines, and that T cell vaccines may be relevant for individuals that cannot produce neutralizing antibodies or to help mitigate Long COVID.

Research on Rural Landscape Spatial Information Recording and Protection Based on 3D Point Cloud Technology under the Background of Internet of Things.

The village with historical and cultural accumulation not only is the witness of the interaction between human activities and natural environment but also contains a lot of intangible cultural heritage. It is an important research object in the history of human social development and has great reference value for the construction of modern Humayun settlements. With the continuous change of modern rural landscape, the traditional engineering drawing cannot meet the needs of people for spatial information measurement. There are some problems such as slow drawing, low precision, and poor benefit, so computer drawing must be used. Therefore, this paper puts forward the research on rural landscape spatial information recording and protection based on 3D point cloud technology under the background of Internet of Things and realizes the recording of rural landscape spatial information through the construction of 3D point cloud spatial information recording model. The experimental results show that the improved unit point cloud spatial information recording model has better feature extraction effect and feature point extraction efficiency, and can classify the point cloud more carefully. At the same time, the scene 3D point cloud classification method based on conditional random field can better deal with the interference factors in the outdoor landscape and show a better classification effect.

Advance in chin-down maneuver in aspiration for dysphagic patients (review) / 中国康复理论与实践

ObjectiveTo identify the appropriate definition, scope of application, the mechanism and the efficacy of the chin-down maneuver in aspiration reduction or prevention. MethodsLiteratures about chin-down published before July, 2021 were reviewed. ResultsA total of 13 articles were included. Chin-down refered to the action of bending the neck and leaning the chin as far as possible toward the chest. Chin-down maneuver could change the position and pressure of the pharyngeal structure to guide the food safely into the esophagus, to prevent or reduce aspiration. Chin-down maneuver was effctive on dysphagia caused by insufficient laryngeal elevation, delayed pharyngeal reflex, epiglottal defect, lychee cartilage defect, and glottis closure defect. However, this action worked in patients with premature overflow of food clumps, weak pharyngeal contraction muscle strength, and poor oral preservation. ConclusionChin-down maneuver is beneficial to aspiration dysphagia after dysphagia.

Preliminary Immunogenicity of a Pan-COVID-19 T Cell Vaccine in HLA-A*02:01 Mice

New strains of SARS-CoV-2 have emerged, including B.1.351 and P.1, that demonstrate increased transmissibility and the potential of rendering current SARS-CoV-2 vaccines less effective. A concern is that existing SARS-CoV-2 spike subunit vaccines produce neutralizing antibodies to three dimensional spike epitopes that are subject to change during viral drift. Here we provide an initial report on the hypothesis that adaptive T cell based immunity may provide a path for a pan-COVID-19 vaccine that is resilient to viral drift. T cell based adaptive immunity can be based on short peptide sequences selected from the viral proteome that are less subject to drift, and can utilize multiple such epitopes to provide redundancy in the event of drift. We find that SARS-CoV-2 peptides contained in a mRNA-LNP T cell vaccine for SARS-CoV-2 are immunogenic in mice transgenic for the human HLA-A*02:01 gene. We plan to test the efficacy of this vaccine with SARS-CoV-2 B.1.351 challenge trials with HLA-A*02:01 mice.

Progress in feeding recommendations for small for gestational age infants / 国际儿科学杂志

As a special group of newborns, small for gestational age (SGA) has high morbidity and mortality in perinatal period, and survivors often leave many health problems and sequelae.Early active nutritional support is beneficial to improve physical growth and nervous system development, and improve immunity, but rapid growth is also associated with obesity, insulin resistance, metabolic syndrome and so on.How to balance the advantages and disadvantages and achieve optimal catch-up growth is an important challenge for SGA nutritional support.This paper reviews the characteristics of SGA growth and development, nutritional requirements, feeding strategies and growth rate, in order to provide a basis for further research on feeding guidelines for children younger than gestational age.

Establishment of Geographic Information System on risk assessment regarding infectious diseases imported to China / 中华流行病学杂志

Objectives@#To establish a geographic information application system for analyzing the spatial and temporal distribution of major infectious diseases in various regions of the world and to assess the risk of importation of those diseases, to China.@*Methods@#We collected and integrated the following information on: 1) outbreaks and areas of epidemics of major infectious diseases in the world from 2000 to 2017, 2) cases of infectious diseases in arriving travelers through active surveillance at international entry-exit ports in mainland China from 2014 to 2016, 3) numbers of annual global international flights and travelers in the country. With the above information, a global space-time distribution database on major infectious diseases was then established, using the technology related to the system. Models regarding technologies on time-space analysis, probabilistic risk assessment and geographic information visualization, were applied to establish a geographic information system on risk assessment of infectious diseases that imported to China.@*Results@#Through integration of information on outbreaks and epidemic areas of 60 major infectious diseases in 220 countries and regions around the world, as well as 42 kinds of infectious diseases identified among the international arrivals in mainland China, a system was then developed. Information on the distribution of major infectious diseases and their potential risks in the worldwide various regions, characteristics of spectrum and disease burden of infectious diseases imported to each province of mainland China were displayed. Thus, risks on importing infectious diseases in each province via air way were able to be evaluated and simulated by the probabilistic risk assessment model, under the information on specific kind of infectious disease, outside China.@*Conclusion@#Geographic Information System on Risk Assessment Regarding Infectious Diseases Imported to China provides basic data for epidemiological reconnaissance and assessment on risks of importing infectious diseases outside China, thus would be helpful for the improvement of strategies on surveillance, prevention and control regarding the importing infectious diseases, in China.

Clinical effect of Xingnao acupuncture method and swallowing rehabilitation training in the treatment of dysphagia after stroke / 中国基层医药

Objective To explore the effect of Xingnao acupuncture combined with swallowing rehabilitation training on dysphagia after stroke .Methods 66 patients with dysphagia after stroke were selected as the research subjects ,and they were randomly divided into the observation group and the control group ,33 cases in each group .The control group was given rehabilitation training on the basis of conventional treatment ,while the observation group was given Xingnao acupuncture method on the basis of the control group .The therapeutic effect ,swallowing function and adverse reactions were observed before and after treatment in the two groups .Results After treatment,the treatment effect in the observation group was significantly better than that in the control group ,there was statistically significant difference between the two groups (Z=-4.123,P<0.05).The total effective rates of the two groups were 93.94％, 75.76％,respectively,there was no statistically significant difference between the two groups (χ2 =1.906,P>0.05). After treatment,the Watian drinking water test scores,standardized swallowing assessment (SSA) score,dysphagia score in the control group were (1.39 ±0.47) points,(23.02 ±5.24) points,(1.25 ±0.55) points,respectively, which in the observation group were (0.74 ±0.39) points,(18.26 ±3.71) points,(0.74 ±0.28) points,and the differences were statistically significant compared with before treatment ( the control group:t =21.453,10.644, 26.212,all P<0.05;the observation group:t=27.779,14.15,37.469,all P<0.05),which in the observation group were better than those in the control group ,the differences were statistically significant ( t=6.114,4.259,4.747,all P<0.05).There was no statistically significant difference in the incidence of adverse events between the two groups (P>0.05).Conclusion Xingnao acupuncture combined with swallowing rehabilitation training in the treatment of dysphagia after stroke can effectively improve the swallowing function ,improve the quality of life of patients ,and it is safe,reliable and worthy of promotion in clinical practice .

Analysis of influence factors on anemia in preterm infants / 临床儿科杂志

Objective To study the influencing factors on anemia in preterm infants at the corrected age of 6-month-old based on gestational age (GA),birth weight (BW) and feeding pattems.Method Preterm infants with GA＜37 weeks (n=124)were followed up to 6 months of corrected age (CA) between June 2014 and November 2015.The incidence of anemia in preterm infants among different groups according to GA,BW and feeding pattem was statistically analyzed.Results Preterm infants included are of 70 males and 54 females.Median age of GA was 33.7 weeks with an average BW of 1910g.The incidences of anemia was 30.6％ (95％ CI:23％,38％) in preterm infants at 6 months of CA,66％ in breast-fed preterm infants.which is significantly higher than 19％ in those receiving mixed feeding and 13％ in those receiving formula feeding (P=0.000).No significant differences in anemia incidence were found among preterm infants of different GA and BW.Conclusions Anemia incidence in 6-month-old preterm infants is associated with feeding pattern,which conferrs greatest risk.It is necessary to undergo further analysis and treatments for preterm infants with anemia.

Comparison in physical and neurological development between two types of children born small for gesta-tional age / 国际儿科学杂志

Almost 3% of newborns are diagnosed as small for gestational age(SGA)worldwide.Born SGA is one of the important causes of perinatal morbidity and mortality,and is also associated with metabolic diseases in adulthood.Low birth weight by itself is insufficient to characterize growth restriction,as it does not include information about the neonate’s body proportionality.Depending on the origin,timing and severity of in-sult,small for gestational age infants are classified into two types:proportionate or symmetric growth restriction (SGR)and disproportionate or asymmetric growth restriction(AGR).There may be differences in physical and neurological development of these two types.This study compares three classification indexes,and to find differ-ences in postnatal growth of these two types.

Effect of diltiazem in patients with coronary disease following percutaneous coronary intervention / 中国基层医药

Objective To observe the clinical efficacy of diltiazem in patients with coronary disease following percutaneous coronary intervention(PCI).Methods From Jan 2014 to Jan 2016,116 cases of coronary disease were selected as the research subjects,they were randomly divided into the observation group(62cases)and the control group(54 cases).The control group was given conventional therapy,while the observation group was given diltiazem therapy on the basis of control group.The levels of BP,HR,NT -proBNP,CRP and angina frequency in the two groups were compared before and 24h,48h after PCI.The incidence of major adverse cardiovascular events(MACEs) was assessed at the sixth month after PCI.Results After surgery,the CRP levels of the observation group and control group were (40.1 ±7.4)mg/L and (41.7 ±8.8)mg/L,the NT -proBNP levels of the two groups were (501.8 ± 56.5)ng/L and (445.7 ±50.6)ng/L,which were significantly higher than preoperation(t =5.684,P <0.05).The blood pressure,heart rate,CRP,NT -proBNP of the observation group since 24 hours after PCI were (73.1 ± 6.2)mmHg,(121.7 ±15.8)mmHg,(68.1 ±7.9)times/min,(24.8 ±3.7)mg/L,(201.7 ±20.6)ng/L,which were significantly lower than those of control group [(85.6 ±9.3 )mmHg,(134.3 ±17.4)mmHg,(77.5 ± 9.2)times/min,(36.5 ±7.9)mg/L,(481.5 ±48.2)ng/L].The effect was kept over 48 hours(F =7.281,8.097, 6.945,7.682,6.517,all P <0.05).Angina pectoris after treatment in the observation group and control group were (1.5 ±0.9)times/month,(2.9 ±0.7)times/month,which were significantly lower than before treatment(t =7.584,P <0.05).The incidence rate of total MACE in the observation group was 4.84%,which was significantly lower than that in the control group(χ2 =6.942,P <0.05).Conclusion Diltiazem after PCI can decrease the adverse reaction, and help to improve the prognosis of patients.

Analgesic effect of acupuncture during migraine acute attack period:a Meta analysis / 重庆医学

Objective To evaluate the effect of acupuncture for treating migraine acute attack to offer some evidence‐based basis for clinical application .Methods The Chinese and English literatures on the acupuncture for treating migraine acute attack were retrived from January 1989 to December 2014 ,the literatures were screened according to inclusion and exclusion criteria ,the Meta‐analysis was performed on these chose literatures .Results A total of 5 studies were included and 618 migraineurs were in‐volved ,four literatares were performed the Meta‐analysis ,and 1 literature was performed the description analysis .Meta‐analysis re‐sults showed that there was statistically significant differences between the acupuncture group and the sham acupuncture group in the VAS score reduction value at 2 h[MD=0 .36 ,95% CI:0 .08 ,0 .65 ,P=0 .01] ,4 h[MD=0 .49 ,95% CI:0 .14 ,0 .84 ,P=0 .007] after acupuncture;while when the VAS score was used as the evaluation indicator ,there was no statistically significant differences were found at 2 h[MD= -0 .38 ,95% CI:-0 .83 ,0 .07 ,P=0 .10] ,4 h[MD= -0 .42 ,95% CI:-0 .96 ,0 .12 ,P=0 .12] after acu‐puncture in the VAS score between the acupuncture group and the sham acupuncture group .Conclusion Acupuncture could effec‐tively relieve the intensity of headache in migraine ,the analgesic effect of acupuncture for treating migraine attacks is significantly superior to the sham acupuncture group ,while with the VAS score as the evaluation indicator ,the difference between the acupunc‐ture group and the sham acupuncture group has no statistical significance .

Distribution characteristics of overweight and obesity in 0-5 years old children born small for gestational age in Shanghai city / 中华实用儿科临床杂志

Objective To understand the distribution of overweight and obesity in 0-5 years old children who were born small for gestational age ( SGA) in Shanghai through a cross-sectional investigation. Methods All resident children aged 0-5 years were included, covering all 18 districts in Shanghai. Health-check cards were prepared for SGA children. The check-up included weight,length/height and head circumference. Body mass index ( BMI) was used to evaluate the overweight and obesity according to the standard of World Health Organization ( WHO) . Results This study investigated 728 602 children aged 0-5 years in Shanghai,and ultimately 23 871 of them were defined as SGA,a-mong whom 9 805(41. 4%) were boys and 14 066(58. 9%) were girls. The BMI of SGA children were higher than that of appropriate for gestational age( AGA) from 4 to 18 months,while for the rest of the time,they were basically the same. There was no difference in changing tendency of BMI between SGA children and those AGA children. The distri-bution of overweight and obesity according to the standard of WHO in SGA boys among different ages was 7. 7% to 20. 7%, and 15. 7% in average;the distribution of SGA girls among different ages was 5. 9% to 18. 3%,and 12. 9% in average. The proportion of overweight at the age of 4-18 months was significantly higher than that of other ages. There was no correlation between overweight at 0-2 years old and overweight at the age of 5(P>0. 05). Conclusions Com-pared with SGA girls,overweight and obesity in SGA boys were more serious. The age of 4-18 months were the period of high incidence of overweight. There was no correlation between overweight at 0-2 years old and overweight at the age of 5.

On-pump coronary artery bypass grafting:a report of 350 cases / 局解手术学杂志

Objective To summarize the clinical experiences of coronary artery bypass grafting ( CABG) in the treatment of coronary artery disease. Methods CABG were performed on 395 consecutive cases from January 2002 to December 2012,including 299 male and 96 female with a mean age of 62. 3 years old. All the operation were performed under cardiopulmonary bypass (CPB ) with moderate hypothermi-a. Left ventricular aneurysm plasty operation were performed in 18 patients. Results The mean number of grafts was 3. 2,the mean CPB time was 88 min( 62~170 min) ,aortic cross-clamping time was 68 min( 25~102 min) ,mean ventilation time was 18 h( 12~72 h) . There were 8 deaths with a mortality of 2. 0%. Six patients died of multiple organ failure,1 patients died of ventricular fibrillation after operation,1 patients died of acute myocardial infarction. Postoperative follow up was carried out on 280 cases,follow-up time was from 8 months to 11 years. Five of them died of unknow causes. The heart function of the rest was significantly improved. 195 patients were free of angina. 85 pa-tients’ s symptom got better. Conclusion CABG performed under cardiopulmonary bypass ( CPB ) with moderate hypothermia is safe and effective for the treatment of coronary artery disease.

Archiater Zhang Nianzhi's Experience in Treating Primary Bronchial Lung Cancer with Method of Supporting Essence to Remove Pathogeny / 浙江中医药大学学报

[Objective]To sum up archiater Zhang Nianzhi's experience in treating lung cancer by supporting essence to remove pahtogeny. [Method] By analysing the causa morbi and mechanism of primary bronchial lung cancer, it expounds Zhang Nianzhi's treating primary bronchial lung cancer by insist-ing on the general rule of strengthening body resistance to eliminate pathogenic factors, applying revised self-made Feiji-1 to the disease, exampled with cases. [Result] Mr Zhang's method centers on nourishing Yin and clearing Qi and removing sputum and stasis, smartly applying Feiji-1 decoction on the disease, with good result. [Conclusion] Mr. Zhang's thought of strengthening body resistance to remove pathogeny, smartly applying self-made Feiji-1 decoction has effective result on clinic.

Study on the treatment of wastewater containing mercury by macromolecular heavy metal flocculant mercaptoacetyl polyethyleneimine.

A novel water-soluble macromolecule heavy metal flocculant mercaptoacetyl polyethyleneimine (MAPEI) was synthesized by reacting polyethyleneimine (PEI) with thioglycolic acid (TGA). The removal of mercury ions (Hg2+) and turbidity from aqueous solutions by MAPEI was investigated. The MAPEI is efficient for the simultaneous removal of Hg2+ and turbidity. The highest removal ratio of mercury ions was above 95%. Factors were studied that could affect the process, such as pH, molecular weight of PEI, co-existing ions, and so on, and the results show that, with the increase in pH or molecular weight of PEI, the removal rate increases; alkali metal and alkaline-earth metal ions and chloride ions (Cl-) and nitrate (NO3-) ions can promote the removal of Hg2+, while sulfate (SO4(2-)) suppresses the process. In the process of treating wastewater containing both turbidity and mercury ions, both have a synergic removal effect with each other. Compared with TGA, MAPEI shows obvious advantages, including higher removal efficiency, larger flocs, and higher floc sedimentation velocity. In addition, compared with PEI, both PEI and MAPEI are almost equally not efficient for turbidity removal, but the presence of mercury ions can improve greatly the efficiency of MAPEI in the removal of turbidity.

Effect of chronic atrial fibrillation on Ca~(2+) /calmodulin dependent protein kinase expression in human atrial myocytes / 第三军医大学学报

Objective To investigate the effect of chronic atrial fibrillation on free calcium concentration and expression of Ca 2+ /calmodulin dependent protein kinase Ⅱ (CaMKⅡ) in human atrial myocytes. Methods The intracellular free calcium concentration in rapidly isolated atrial myocytes and the expression of CaMKⅡ in atrial tissue of rheumatic heart disease patients with atrial fibrillation (AF) or with normal sinus rhythm were measured by laser scanning confocal microscopy and Western blotting respectively. Results The intracellular Ca 2+ concentration of patients with atrial fibrillation was significantly higher than that of patients with normal sinus rhythm [(276.38?38.12) vs (122.28?45.63) nmol/L, (P

Clinical analysis of on-pump, beating-heart intracardiac proc edures in 1 032 cases / 第三军医大学学报

Objective　To improve intracardiac operation skil ls on bea-ting-heart with mild hypothermic cardiopulmonary bypass (On pump beating-heart technique), and to review the clinical experience in 1 032 c ases. Methods　A total of 1 032 cases of intracardiac operatio ns on pump beating-heart from November 1997 to September 2000 were reviewed. Of them, 714 cases were congenital heart diseases (CHD), and 318 cases were valvul ar heart diseases (VHD). The technique was improved by establishing simultaneous left atrium and ventricle suction and integrating sequential de-airing procedu re. Results　The operative mortality was 2.33% (24/1 032), the m ortality was 2.7% (19/714) in cases with CHD, and 1.6% (5/318) in those with VHD. There was no pati ent complicated with systemic air embolism or permanent atrioventricular conduct ion block. Conclusion　Results suggested that intracardiac procedures on pump beating-heart with mild hypothermic cardiopnlmonary bypass is safe and available in patients with CHD or VHD. It might extenuate the heart and lung injury by hypothermia and ischemia-reperfusion during cardiopulmonary bypass. Cardiac conducting block might be prevented during operation.