Subject(s)
Heterozygote , Mice, Nude/immunology , Thymus Gland/immunology , Animals , Bone Marrow Cells/cytology , CD4 Lymphocyte Count , Colony-Forming Units Assay , Colony-Stimulating Factors/analysis , Femur , Hematopoietic Stem Cells/cytology , Mice , Spleen/immunology , Spleen/pathology , Thymus Gland/pathologyABSTRACT
During in vitro incubation, the pulmonary and thymic tissue of mice released a colony stimulating factor (CSF) supporting the development of colonies of granulocytes and macrophages from bone marrow progenitors. Cardiac, splenic, renal and bone marrow tissues were not active. The dysgenetic thymus of nude mice was a very potent source of CSF during in vitro incubation.
Subject(s)
Granulocyte-Macrophage Colony-Stimulating Factor/isolation & purification , Thymus Gland/metabolism , Aging , Animals , Cells, Cultured , Culture Media , Lung/metabolism , Lung/ultrastructure , Mice , Mice, Inbred Strains , Mice, Nude , Organ Culture Techniques , Thymus Gland/ultrastructureABSTRACT
Random-bred ICR mice exhibited a wide range of spleen colony-forming units (CFU-S) and granulocyte-macrophage colony-forming cells (GM-CFC) between individuals. Both CFU-S and GM-CFC values were, however, relatively stable in individual mice over a period of 6 weeks, although the cellularity of the marrows changed significantly during the same period. CFU-S and GM-CFC values were only weakly correlated in individual mice. Bone marrows of mice with low CFU-S values did not have a lower cellularity than mice with high CFU-S values. A low level of CFU-S thus appeared to be compensated for by a higher clonal expansion of maturing cells. It may be concluded that there is wide variation in the organization of the stem cell compartment. Individuals may thus differ markedly in the organization of the marrow. Such differences may prevail in man and be of functional importance at times of extra demands, e.g., during cytostatic therapy.
Subject(s)
Hematopoietic Stem Cells/cytology , Animals , Colony-Forming Units Assay , Female , Granulocytes/cytology , Macrophages/cytology , Mice , Mice, Inbred C57BL , Mice, Inbred ICR , Spleen/cytologyABSTRACT
Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during the last phase the decrease became stabilized at a value which differed significantly from the maximum (P less than 0.01). The cause of variability in the number of positively reacting cells in relation to the exposure time is discussed.
Subject(s)
Epithelium/microbiology , Streptococcus agalactiae/physiology , Bacteriological Techniques , Cells, Cultured , Female , Humans , Mouth Mucosa/microbiology , Time Factors , Vagina/microbiologyABSTRACT
Proof of adherence of group B streptococci (GBS) to human and bovine vaginal epithelial cells and to bovine cells of milk cisternae of the mammary gland was employed as a criterion determining the possibility of colonization of these organs with GBS, or as another method of testing the transfer of GBS between man and cattle. GBS of both human and animal origin adhered to human epithelial cells in a similar way. On the other hand, a significantly stronger adherence of bovine GBS to vaginal epithelial cells and cells of milk cisternae of cattle was found than of human GBS. Thus the direction of colonization - animal is more probable than the opposite way. Neither in animal nor in human strains a correlation between the equipment of strains with type antigens and intensity of adherence could be found.
Subject(s)
Cattle/microbiology , Streptococcus agalactiae/physiology , Vagina/microbiology , Animals , Epithelium/physiology , Female , Genitalia, Female/microbiology , Humans , Mammary Glands, Animal/cytology , Mastitis, Bovine/transmission , Milk/microbiology , Pregnancy , Species Specificity , Streptococcal Infections/transmission , Streptococcus agalactiae/isolation & purification , Vagina/cytologyABSTRACT
Hydrogen ion concentration in a medium in which the adherence of group B streptococci to vaginal and buccal cells takes place, significantly influences the reaction intensity. At physiological pH, group B streptococci adhere significantly more weakly than at pH 5.5 to buccal epithelia, and at pH 7.2 to vaginal epithelia. Thus at nonphysiological pH values the percentage of adherent cells is markedly higher.
