1.
Plant Mol Biol
; 14(2): 111-7, 1990 Feb.
Article
in English
| MEDLINE
| ID: mdl-2101685
ABSTRACT
The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species.
Subject(s)
DNA, Bacterial/genetics , Plant Tumors/microbiology , Rhizobium/genetics , DNA Probes , DNA, Bacterial/analysis , Genetic Vectors , Plants/genetics , Plasmids , Restriction Mapping , Rhizobium/pathogenicity , Transfection , Trees
2.
Tandlakartidningen
; 77(12): 675-7, 1985 Jun 15.
Article
in Swedish
| MEDLINE
| ID: mdl-3865438