Effects of an 8-Week Dairy Production Medicine Course on Veterinary Student Self-Confidence and Perceptions of Knowledge and Skills Used by Dairy Veterinarians.

The 8-week dairy production medicine course at the National Center of Excellence in Dairy Production Medicine Education for Veterinarians is designed to equip senior veterinary students with the knowledge and skills needed to serve the dairy industry. Course developers identified 59 topics of importance for dairy production medicine veterinarians. Students (N = 50) were surveyed before and after the course to determine their perceptions of (a) the importance of the 59 topics for their intended positions and (b) their knowledge and skill in those areas. We expected the course to affirm or strengthen perceptions of importance and increase confidence. Students rated 57 of the topics as moderately or very important before the course. Ratings were unchanged (56 topics) or increased (3 topics) after the course. Before the course, students believed they had a lot of knowledge and skill in just one area: animal behavior and handling. At the end of the course, students believed they had a lot of knowledge and skill in 21 areas; confidence ratings were higher for 47 of the 59 topics. Alumni were surveyed 1-2 years after graduation to determine the importance of the 59 topics to their positions, their impressions about how well the course prepared them in those areas, and whether they referred back to course materials. Feedback was used to adjust the course. The topics alumni rated as most important were similar to those students predicted would be most important. Seventy-five percent of alumni used the course website as a resource in practice.

A Pilot Study of Chicago Waterways as Reservoirs of Multidrug-Resistant Enterobacteriaceae (MDR-Ent) in a High-Risk Region for Community-Acquired MDR-Ent Infection in Children.

Community-acquired multidrug resistant Enterobacteriaceae (MDR-Ent) infections continue to increase in the United States. In prior studies, we identified neighboring regions in Chicago, Illinois, where children have 5 to 6 times greater odds of MDR-Ent infections. To prevent community spread of MDR-Ent, we need to identify the MDR-Ent reservoirs. A pilot study of 4 Chicago waterways for MDR-Ent and associated antibiotic resistance genes (ARGs) was conducted. Three waterways (A1 to A3) are labeled safe for "incidental contact recreation" (e.g., kayaking), and A4 is a nonrecreational waterway that carries nondisinfected water. Surface water samples were collected and processed for standard bacterial culture and shotgun metagenomic sequencing. Generally, A3 and A4 (neighboring waterways which are not hydraulically connected) were strikingly similar in bacterial taxa, ARG profiles, and abundances of corresponding clades and genera within the Enterobacteriaceae Additionally, total ARG abundances recovered from the full microbial community were strongly correlated between A3 and A4 (R2 = 0.97). Escherichia coli numbers (per 100 ml water) were highest in A4 (783 most probable number [MPN]) and A3 (200 MPN) relative to A2 (84 MPN) and A1 (32 MPN). We found concerning ARGs in Enterobacteriaceae such as MCR-1 (colistin), Qnr and OqxA/B (quinolones), CTX-M, OXA and ACT/MIR (beta-lactams), and AAC (aminoglycosides). We found significant correlations in microbial community composition between nearby waterways that are not hydraulically connected, suggesting cross-seeding and the potential for mobility of ARGs. Enterobacteriaceae and ARG profiles support the hypothesized concerns that recreational waterways are a potential source of community-acquired MDR-Ent.

Prior Experience, Career Intentions, and Post-Graduate Positions of Veterinary Students Who Participated in an 8-week Dairy Production Medicine Course.

Three cohorts of senior veterinary students (n = 50) from seven United States (US) colleges of veterinary medicine took an 8-week dairy production medicine course at the Dairy Center of Excellence in Production Medicine Education for Veterinarians (DCE) between 2012 and 2014. Participants completed a questionnaire before and after the course and 1 to 2 years after graduation. Objectives were to determine the prior academic training and livestock experience of course participants, to compare students' career aspirations before and after taking the course, and to identify factors associated with post-graduate position. Response rates were 58%-96%. Most students had taken undergraduate animal science courses (83%), worked (76%) and/or lived (52%) on a livestock operation, participated in youth livestock activities (63%), worked at a mixed practice (71%), taken production medicine-related elective courses (65%), taken other food animal rotations (91%), and/or done dairy externships (65%) before taking the DCE course. Students who were very likely to pursue a dairy-focused position before taking the course (36%) remained committed after the course, whereas students who were not likely initially (39%) were not further motivated by the course. Students who had worked with a dairy veterinarian were more likely to pursue a dairy-focused position than those who had not. Most course alumni accepted positions in mixed practice, with a ≥ 50% (54%) or < 50% (23%) dairy component, and post-graduate positions were consistent with students' predictions. Students who held an undergraduate degree or had worked for a dairy veterinarian were more likely to accept a dairy-focused practice position than those who did not.

Considerations for studying transmission of antimicrobial resistant enteric bacteria between wild birds and the environment on intensive dairy and beef cattle operations.

BACKGROUND: Wild birds using livestock facilities for food and shelter may contribute to dissemination of enteric pathogens or antimicrobial resistant bacteria. However, drivers of microbial exchange among wildlife and livestock are not well characterized. Predisposition for acquiring and retaining environmental bacteria may vary among species because of physiologic or behavioral differences, complicating selection of a bacterial model that can accurately characterize microbial connections among hosts of interest. This study compares the prevalence and antibiotic resistance phenotypes of two potential model bacterial organisms isolated from wild birds and their environments. METHODS: We compared prevalence and resistance profiles of Escherichia coli and Enterococcus species isolated from environmental swabs and bird feces on a residential control site, a confinement dairy, a pasture-based beef farm, and a confinement beef farm. RESULTS: Bird feces at all sites had low-to-moderate prevalence of Escherichia coli (range: 17-47%), despite potential for exposure on farms (range: 63-97%). Few Escherichia coli were isolated from the control environment. Enterococcus faecalis was dominant in birds at both beef farms (62% and 81% of Enterococcus isolates) and low-to-moderately prevalent at the dairy and control sites (29% and 23% of isolates, respectively). Antimicrobial resistance prevalence was higher in farm samples compared to those from the residential control, but distribution of resistant isolates varied between the bacterial genera. Birds on all farms carried resistant Enterococcus at similar rates to that of the environment, but resistance was less common in bird-associated Escherichia coli despite presence of resistant isolates in the farm environment. DISCUSSION: Bacterial species studied may affect how readily bacterial exchange among populations is detected. Selection of microbial models must carefully consider both the questions being posed and how findings might influence resulting management decisions.

Di (2-ethylhexyl) phthalate (DEHP) alters proliferation and uterine gland numbers in the uteri of adult exposed mice.

Di-(2-ethylhexyl) phthalate (DEHP) is an endocrine-disrupting chemical that has been shown to impair normal reproductive function in males and females. This study investigated whether adult exposure to environmental and occupational doses of DEHP alters homeostasis of uterine proliferation, morphology, and number of uterine glands. Adult female CD1 mice were orally dosed with DEHP (0, 20 µg/kg/day, 200 µg/kg/day, 20 mg/kg/day or 200 mg/kg/day) for 30 days. Results indicated that DEHP at 200 µg/kg/day caused a reduction in epithelial cell proliferation in the uterus (p < .05). We also observed an increase (p < .05) in the number of uterine glands in mice dosed with 200 mg/kg/day DEHP. Results showed that DEHP caused an increase (p < .05) in dilated blood vessels in the endometrium at 200 µg/kg/day, 20 mg/kg/day and 200 mg/kg/day. DEHP also increased proliferation of endometrial stromal cells at 200 µg/kg/day DEHP (p < .0010), 20 mg/kg/day DEHP (p < .0001) and 200 mg/kg/day DEHP (p < .0186). Results suggest that, exposure to specific doses of DEHP for 30 days can have adverse effects on reproductive function.