Bacillus subtilis BY-kinase PtkA controls enzyme activity and localization of its protein substrates.

Bacillus subtilis BY-kinase PtkA was previously shown to phosphorylate, and thereby regulate the activity of two classes of protein substrates: UDP-glucose dehydrogenases and single-stranded DNA-binding proteins. Our recent phosphoproteome study identified nine new tyrosine-phosphorylated proteins in B. subtilis. We found that the majority of these proteins could be phosphorylated by PtkA in vitro. Among these new substrates, single-stranded DNA exonuclease YorK, and aspartate semialdehyde dehydrogenase Asd were activated by PtkA-dependent phosphorylation. Because enzyme activity was not affected in other cases, we used fluorescent protein tags to study the impact of PtkA on localization of these proteins in vivo. For several substrates colocalization with PtkA was observed, and more importantly, the localization pattern of the proteins enolase, YjoA, YnfE, YvyG, Ugd and SsbA was dramatically altered in DeltaptkA background. Our results confirm that PtkA can control enzyme activity of its substrates in some cases, but also reveal a new mode of action for PtkA, namely ensuring correct cellular localization of its targets.

A direct observation instrument for assessment of nurses' patient transfer technique (DINO).

The aim of this project was to develop a direct observation instrument to assess the work technique of nursing personnel during patient transfers and to test the validity and reliability of the instrument. An expert group developed this instrument, called DINO (DIrect Nurse Observation instrument for assessment of work technique during patient transfers), which contains 16 items divided into three phases of a transfer: the preparation, performance and result phases. To quantify the assessments a scoring system was constructed, giving an overall score for each transfer, depending on the level of musculoskeletal health and safety. Four observers assessed 45 patient transfers at hospital wards and showed in an evaluation that the inter-observer reliability and criterion-related validity of DINO was satisfactory. The assessments with the DINO instrument are done directly when the transfer occurs, without costly equipment. Therefore, it has a wide range of applications. For example, when evaluating training in work technique or when identifying an unsafe work technique as a risk factor for musculoskeletal problems in epidemiological studies.

Evaluation of training in patient handling and moving skills among hospital and home care personnel.

The aim of the study was to evaluate the training programme in patient handling and moving skills according to the Stockholm Training Concept. The evaluation focused on work technique, musculoskeletal problems, job strain and the experience of the person being transferred. The training programme consisted of two models of learning: traditional groups and quality circles. Fifty-one people employed at geriatric hospitals and in primary care, participated in the training programme. For the evaluation, the participants were video-recorded in one standardized transfer situation: moving the patient from bed to wheelchair, before and after the training. The participants' work technique during the patient transfer was assessed using seven items. A questionnaire covering individual factors, physical exertion, job strain, and musculoskeletal problems, was filled in by the participants before training and also six months after. The results showed improvements in six of the seven work technique items, directly after training. A decrease in the participants' physical exertion during transfers of patients from 'bed to chair' was shown six months after completion of the training programme. There was no significant decrease in the participants' musculoskeletal problems and job strain after training. Ninety-eight per cent of the participants were positive about having participated in the training. Training in patient handling and moving skills led to improved work technique. The participants improved their transfer technique and experienced less discomfort during the transfer, and patients experienced greater comfort and safety when being transferred.