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Sci Rep ; 8(1): 3481, 2018 02 22.
Article in English | MEDLINE | ID: mdl-29472564

ABSTRACT

The interaction of light with biological tissue has been successfully utilized for multiple therapeutic purposes. Previous studies have suggested that near infrared light (NIR) enhances the activity of mitochondria by increasing cytochrome c oxidase (COX) activity, which we confirmed for 810 nm NIR. In contrast, scanning the NIR spectrum between 700 nm and 1000 nm revealed two NIR wavelengths (750 nm and 950 nm) that reduced the activity of isolated COX. COX-inhibitory wavelengths reduced mitochondrial respiration, reduced the mitochondrial membrane potential (ΔΨm), attenuated mitochondrial superoxide production, and attenuated neuronal death following oxygen glucose deprivation, whereas NIR that activates COX provided no benefit. We evaluated COX-inhibitory NIR as a potential therapy for cerebral reperfusion injury using a rat model of global brain ischemia. Untreated animals demonstrated an 86% loss of neurons in the CA1 hippocampus post-reperfusion whereas inhibitory NIR groups were robustly protected, with neuronal loss ranging from 11% to 35%. Moreover, neurologic function, assessed by radial arm maze performance, was preserved at control levels in rats treated with a combination of both COX-inhibitory NIR wavelengths. Taken together, our data suggest that COX-inhibitory NIR may be a viable non-pharmacologic and noninvasive therapy for the treatment of cerebral reperfusion injury.


Subject(s)
Brain Injuries/radiotherapy , Electron Transport Complex IV/genetics , Infrared Rays/therapeutic use , Reperfusion Injury/radiotherapy , Animals , Brain/pathology , Brain/radiation effects , Brain Injuries/genetics , Brain Injuries/pathology , Electron Transport Complex IV/radiation effects , Glucose/metabolism , Hippocampus/metabolism , Hippocampus/pathology , Hippocampus/radiation effects , Humans , Membrane Potential, Mitochondrial , Mitochondria/genetics , Mitochondria/radiation effects , Neurons/metabolism , Neurons/radiation effects , Oxidation-Reduction/radiation effects , Rats , Reperfusion Injury/genetics , Reperfusion Injury/pathology
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