"A shoulder to lean on during your first year"-An exploration into a Canadian post-secondary institution's peer mentor program for varsity student athletes.

The transition period from high school to post-secondary can be particularly challenging for many, including varsity student-athletes (SAs). To better support SAs through this transitional experience, some institutions have created peer mentor programs. What is unclear, however, is the perceived value of these mentorship programs from the perspectives of multiple stakeholder positions. This paper contributes to the Scholarship of Teaching and Learning by presenting findings of a program evaluation that investigated the perceived value of a peer mentor program to its stakeholders. To accomplish this, semi-structured interviews were conducted with 30 participants to discuss SA's experiences with being a first year student, making the transition from high school to post-secondary studies, and also, to discuss their lived experiences with the peer mentor program developed for SAs. Using the findings from the inductive thematic analyses, the peer mentor program's effectiveness, areas of strengths, and areas of improvement are discussed to better align with the stakeholders' needs and experiences. Findings offer insights into a) the trials and tribulations of the first year SA experience, b) how peer mentor programs can better support SA's transition to post-secondary education, c) the benefits of conducting a program evaluation, and d) strategies to enhance the peer mentor program to better support students' needs.

Provincial and territorial congenital anomalies surveillance: a summary of surveillance programs across Canada. / Surveillance provinciale et territoriale des anomalies congénitales : résumé des programmes de surveillance au Canada.

The Canadian Congenital Anomalies Surveillance Network was established in 2002 to address gaps in the national surveillance of congenital anomalies (CAs) and support the sustainability of high-quality, population-based, CA surveillance systems within provinces and territories. This paper highlights the methodologies of each local CA surveillance system, noting similarities and variabilities between each system, to contribute to enhanced national CA surveillance efforts.

The Canadian Congenital Anomalies Surveillance Network was established in 2002 under the umbrella of the Canadian Perinatal Surveillance System to support highquality, population-based congenital anomalies surveillance systems in Canada. Each local congenital anomalies surveillance system covers diverse populations and geography, operates under different structures and has varying program maturity. Engagement of every jurisdiction is essential for sustaining local and national CA surveillance. Provincial and territorial CA surveillance systems are uniquely positioned to support public health priorities.

Le Réseau canadien de surveillance des anomalies congénitales a été créé en 2002, dans le cadre du Système canadien de surveillance périnatale, afin de soutenir des systèmes de surveillance des anomalies congénitales de haute qualité et fondés sur la population à l'échelle du Canada. Les systèmes locaux de surveillance des anomalies congénitales couvrent des populations et des zones géographiques diverses, fonctionnent selon des structures différentes et ont une maturité variable. La participation de chaque administration est essentielle pour soutenir la surveillance locale et nationale des anomalies congénitales. Les systèmes provinciaux et territoriaux de surveillance des anomalies congénitales sont particulièrement bien placés pour soutenir les priorités en matière de santé publique.

A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes.

Identifying virulence-critical genes from pathogens is often limited by functional redundancy. To rapidly interrogate the contributions of combinations of genes to a biological outcome, we have developed a multiplex, randomized CRISPR interference sequencing (MuRCiS) approach. At its center is a new method for the randomized self-assembly of CRISPR arrays from synthetic oligonucleotide pairs. When paired with PacBio long-read sequencing, MuRCiS allowed for near-comprehensive interrogation of all pairwise combinations of a group of 44 Legionella pneumophila virulence genes encoding highly conserved transmembrane proteins for their role in pathogenesis. Both amoeba and human macrophages were challenged with L. pneumophila bearing the pooled CRISPR array libraries, leading to the identification of several new virulence-critical combinations of genes. lpg2888 and lpg3000 were particularly fascinating for their apparent redundant functions during L. pneumophila human macrophage infection, while lpg3000 alone was essential for L. pneumophila virulence in the amoeban host Acanthamoeba castellanii. Thus, MuRCiS provides a method for rapid genetic examination of even large groups of redundant genes, setting the stage for application of this technology to a variety of biological contexts and organisms.

Maturity-Associated Polygenic Profiles of under 12-16-Compared to under 17-23-Year-Old Male English Academy Football Players.

The purpose of this study was to examine polygenic profiles previously associated with maturity timing in male academy football players across different age phases. Thus, 159 male football players from four English academies (U12-16, n = 86, aged 13.58 ± 1.58 years; U17-23, n = 73, aged 18.07 ± 1.69 years) and 240 male European controls were examined. Polygenic profiles comprised 39 single nucleotide polymorphisms and were analysed using unweighted and weighted total genotype scores (TGSs; TWGSs). There were significant differences in polygenic profiles between groups, whereby U17-23 players had more genetic variants associated with later maturity compared to U12-16 players (TGS, p = 0.010; TWGS, p = 0.024) and controls (TGS, p = 0.038; TWGS, p = 0.020). More specifically, U17-23 players had over two-times the odds of possessing >36 later-maturing alleles than <30 compared to U12-16 players (odds ratio (OR) = 2.84) and controls (OR = 2.08). These results suggest there was a greater proportion of relatively later-maturing players as maturation plateaus towards adulthood, which may be explained by the 'underdog hypothesis'. This study provides the first known molecular evidence that supports the notion that a maturity selection bias exists within male academy football.

On the predictive validity of the National Football League combine: does it forecast future success?

The National Football League (NFL) Combine provides NFL teams the ability to assess prospective athletes' medical histories and physical and psychological abilities. Using this information, NFL personnel must then decide whether an athlete is a good fit to their team. Given the combine's 40-year history and the availability of peer reviewed articles on the function and efficacy of the combine, the purpose of this systematic review was to synthesize the literature evaluating the predictive validity of the combine according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). Full-text, peer-reviewed articles containing information relevant to the NFL combine and at least one measure of future success were retained. The search yielded 1954 articles and after screening, 68 articles remained. These remaining articles focused on measures of success pertaining to (a) medical testing and future performance (n = 25), (b) combine tests (n = 12), (c) draft position (n = 10), (d) draft and future performance (n = 8), (e) medical testing (n = 7), (f) career longevity (n = 3), (g) draft and salary (n = 2), and (h) playing performance (n = 1). Due to the mixed results of combine measures on future success, this review highlights the need for more research to investigate the combine's influence on long-term performance and success.

Excellence fulfilled? On the unique developmental needs of professional athletes.

While the term "athlete development" has been used to capture the changes (physical, psychological, etc.) that occur as an athlete moves from initial sport engagement to elite performance, much of the research in this area has focused on earlier stages of the pathway, with very little work examining the highest levels of sport. Considering a person's bio-psycho-social development continues through adulthood, the limited attention to development for athletes at higher competitive levels is perhaps surprising. In this short article, we highlight several notable discrepancies between different competitive levels (e.g., pre-professional sport and professional sport) in the way development is conceptualized, contextualized, and operationalized. We use available evidence to provide guidance for researchers and practitioners to encourage the delivery of structured developmental programming in professional sport systems to aid with the transitionary period between pre-elite and elite levels, and to help foster career longevity.

Language games and blurry terminology: Can clarity enhance athlete development?

This perspective focuses on the need for researchers and practitioners to carefully consider the clarity and consistency of their language in the context of athlete development. Evidence supporting a lack of congruency in the way certain terms and expressions are defined, understood, and operationalized continues to accumulate, highlighting the importance of this area for sport stakeholders and the potential looming crises. In systems that regularly rely on precision and accuracy, it will be critical that all involved in the co-creation of knowledge generation and application carefully consider terms that may further complicate athlete development practices. We highlight some potentially blurry terms and draw attention to potential avenues for future research.

Lifespan models of athlete development: What have we learned from previous attempts?

Sport has a unique place in many cultures, emphasizing the links between physical elements of movement with psychological and social outcomes. Sport participation continues to attract the interest of researchers from a range of perspectives, yet there remains a strong need to understand the "who", "what", "where", "when" and "why" aspects of sport involvement over the life course. While the research literature includes multiple athlete development models that consider these components, they are incomplete frameworks for understanding lifespan sport engagement. In this article, we discuss the value in building multidimensional developmental models of sport participation that encapsulate experiences across all ages and stages of competitive and recreational sport, and pay special attention to the high degree of complexity of the movement between and within sport both competitively and recreationally. In addition, we highlight several challenges to creating such a lifespan development model, and consider areas of future direction to overcome some of these hurdles.

Inflammation and DKK1-induced AKT activation contribute to endothelial dysfunction following NR2F2 loss.

NR2F2 is expressed in endothelial cells (ECs) and Nr2f2 knockout produces lethal cardiovascular defects. In humans, reduced NR2F2 expression is associated with cardiovascular diseases including congenital heart disease and atherosclerosis. Here, NR2F2 silencing in human primary ECs led to inflammation, endothelial-to-mesenchymal transition (EndMT), proliferation, hypermigration, apoptosis-resistance, and increased production of reactive oxygen species. These changes were associated with STAT and AKT activation along with increased production of DKK1. Co-silencing DKK1 and NR2F2 prevented NR2F2-loss-induced STAT and AKT activation and reversed EndMT. Serum DKK1 concentrations were elevated in patients with pulmonary arterial hypertension (PAH) and DKK1 was secreted by ECs in response to in vitro loss of either BMPR2 or CAV1, which are genetic defects associated with the development of PAH. In human primary ECs, NR2F2 suppressed DKK1, whereas its loss conversely induced DKK1 and disrupted endothelial homeostasis, promoting phenotypic abnormalities associated with pathologic vascular remodeling. Activating NR2F2 or blocking DKK1 may be useful therapeutic targets for treating chronic vascular diseases associated with EC dysfunction.NEW & NOTEWORTHY NR2F2 loss in the endothelial lining of blood vessels is associated with cardiovascular disease. Here, NR2F2-silenced human endothelial cells were inflammatory, proliferative, hypermigratory, and apoptosis-resistant with increased oxidant stress and endothelial-to-mesenchymal transition. DKK1 was induced in NR2F2-silenced endothelial cells, while co-silencing NR2F2 and DKK1 prevented NR2F2-loss-associated abnormalities in endothelial signaling and phenotype. Activating NR2F2 or blocking DKK1 may be useful therapeutic targets for treating vascular diseases associated with endothelial dysfunction.

A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes.

Identifying virulence-critical genes from pathogens is often limited by functional redundancy. To rapidly interrogate the contributions of combinations of genes to a biological outcome, we have developed a multiplex, randomized CRISPR interference sequencing (MuRCiS) approach. At its center is a new method for the randomized self-assembly of CRISPR arrays from synthetic oligonucleotide pairs. When paired with PacBio long-read sequencing, MuRCiS allowed for near-comprehensive interrogation of all pairwise combinations of a group of 44 Legionella pneumophila virulence genes encoding highly conserved transmembrane proteins for their role in pathogenesis. Both amoeba and human macrophages were challenged with L. pneumophila bearing the pooled CRISPR array libraries, leading to the identification of several new virulence-critical combinations of genes. lpg2888 and lpg3000 were particularly fascinating for their apparent redundant functions during L. pneumophila human macrophage infection, while lpg3000 alone was essential for L. pneumophila virulence in the amoeban host Acanthamoeba castellanii. Thus, MuRCiS provides a method for rapid genetic examination of even large groups of redundant genes, setting the stage for application of this technology to a variety of biological contexts and organisms.

Sources of information used by elite distance running coaches for selection decisions.

Talent identification and selection are critical components of competitive sport success. Despite the time, effort, and resources invested, the accuracy of selection decisions remains generally poor. While much of the scholarship in this area has focused on the factors discriminating skilled and less-skilled individuals, limited research exists on what information is used in the decision-making process for athlete selection. The current study seeks to gain a better understanding of the information used by elite distance running coaches when forming judgements for athlete selection. Ten semi-structured interviews with elite distance running coaches from across Canada were transcribed and analyzed using inductive thematic analysis. It was interpreted that coaches mainly gather information using their coach's eye to determine an athlete's 'fit' to the team. Coaches also use more objective information such as race times and movement analyses to assess performance and judge future 'potential'. As well, the decisions were believed to be influenced by situational considerations at the time of the selection procedure. Specifically, these considerations affecting a coach's selection included length of time to make a decision, personal limitations in decision-making abilities, and team circumstances. Interestingly, coaches recognized limitations in their selection practices and procedures and discussed some of their personal and system-level biases, highlighting their awareness of potential selection inefficiencies/inaccuracies. Overall, distance running coaches used a variety of techniques to gather information before a selection was made, relying on both subjective and objective information for crafting judgments. Findings are discussed in relation to implications for coaches, sport organizations, and talent identification and selection programs.

Mineralocorticoid receptor antagonist treatment of established pulmonary arterial hypertension improves interventricular dependence in the SU5416-hypoxia rat model.

Treatment with mineralocorticoid receptor (MR) antagonists beginning at the outset of disease, or early thereafter, prevents pulmonary vascular remodeling in preclinical models of pulmonary arterial hypertension (PAH). However, the efficacy of MR blockade in established disease, a more clinically relevant condition, remains unknown. Therefore, we investigated the effectiveness of two MR antagonists, eplerenone (EPL) and spironolactone (SPL), after the development of severe right ventricular (RV) dysfunction in the rat SU5416-hypoxia (SuHx) PAH model. Cardiac magnetic resonance imaging (MRI) in SuHx rats at the end of week 5, before study treatment, confirmed features of established disease including reduced RV ejection fraction and RV hypertrophy, pronounced septal flattening with impaired left ventricular filling and reduced cardiac index. Five weeks of treatment with either EPL or SPL improved left ventricular filling and prevented the further decline in cardiac index compared with placebo. Interventricular septal displacement was reduced by EPL whereas SPL effects were similar, but not significant. Although MR antagonists did not significantly reduce pulmonary artery pressure or vessel remodeling in SuHx rats with established disease, animals with higher drug levels had lower pulmonary pressures. Consistent with effects on cardiac function, EPL treatment tended to suppress MR and proinflammatory gene induction in the RV. In conclusion, MR antagonist treatment led to modest, but consistent beneficial effects on interventricular dependence after the onset of significant RV dysfunction in the SuHx PAH model. These results suggest that measures of RV structure and/or function may be useful endpoints in clinical trials of MR antagonists in patients with PAH.

Survival Versus Attraction Advantages and Talent Selection in Sport.

Athlete selection (often referred to as talent selection) reflects the end point of what is a complex decision-making process coaches, administrators, and/or scouts use when deciding who remains and who is removed from a sample of potential athletes. In this paper, we conceptualize athlete selection as an evolutionary process where selection pressures (e.g., performance demands, system limitations) influence the value of one trait/characteristic over another. Athletes are selected either through demonstrating enhanced performance (survival advantages) or by having characteristics that are desirable to the coach/recruiter making the selection (attraction advantages). Based on these varying pressures, our understanding of whether profiles of current athletes represent the actual elements of performance necessary for success or simply those most needed for selection at key points in athlete development is extremely limited.

To draft or not to draft? A systematic review of North American sports' entry draft.

In theory, professional sport "entry drafts" are designed to promote parity by granting poorly performing teams with early selections and winning teams with later selections. While this process has intentions to "level the playing field", mixed findings exist in the literature. The aim of this review is to identify and synthesize the literature examining the efficacy of the draft for professional, North American sport leagues. A systematic review of four databases was performed according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines. Full-text articles containing relevant data on the draft system for the four major professional North American sports were identified. Further restrictions were made to include articles focusing on a specific outcome regarding future success (i.e., whether the draft related to a measure of future performance). The search returned 10 962 records and after screening, 18 articles were synthesized. Of the articles examined, the measures of future success with relation to draft order were (a) career length and/or number of games played at the majors (n = 8), (b) future performance statistics at the professional level (n = 5), (c) change in winning percentage and/or number of wins produced (n = 3), (d) financial compensation (n = 1), and (e) a combination of measures (a) to (d), (n = 1). Most commonly, the first/early rounds most accurately predicted future measures of success (ie, number of games played, signing bonuses, and playing statistics) across sports. The middle and late rounds were less accurate, with the degree of accuracy increasing slightly in the last rounds. This review highlights several opportunities to better understand the draft process (e.g., potential improvements in middle round picks) and emphasizes the need for more research on analyzing and scrutinizing the draft.

Predicting Seasonal Performance in Professional Sport: A 30-Year Analysis of Sports Illustrated Predictions.

In 2017, Sports Illustrated (SI) made headlines when their remarkable prediction from 2014 that the Houston Astros (a team in one of the lowest Major League Baseball divisional rankings) would win the World Series, came true. The less-publicised story was that in 2017, SI predicted the Los Angeles Dodgers to win the Major League Baseball (MLB) title. Assessing the forecasting accuracy of experts is critical as it explores the difficulty and limitations of forecasts and can help illuminate how predictions may shape sociocultural notions of sport in society. To thoroughly investigate SI's forecasting record, predictions were collected from the four major North American sporting leagues (the National Football League, National Basketball Association, Major League Baseball, and National Hockey League) over the last 30 years (1988-2018). Kruskal-Wallis H Tests and Mann-Whitney U Tests were used to evaluate the absolute and relative accuracy of predictions. Results indicated that SI had the greatest predictive accuracy in the National Basketball Association and was significantly more likely to predict divisional winners compared to conference and league champions. Future work in this area may seek to examine multiple media outlets to gain a more comprehensive perspective on forecasting accuracy in sport.

Storm Clouds on the Horizon: On the Emerging Need to Tighten Selection Policies.

Athlete selection is fundamental in elite sport, occurring regularly throughout an athlete's development. Research in this area reveals the accuracy of these decisions is questionable in even the most elite sport environments and athletes are increasingly disputing these decisions as unfair and punitive. As a countermeasure to these dispute and arbitration practices, many elite sport systems have created policies where coaches must outline and stand behind the criteria used for their selection decisions. Selection criteria policies have the potential to help encourage fair selection practices by holding selectors accountable to their selection criteria, but their implementation also has the potential to wrongfully nudge selectors toward developing more defendable, but less-accurate selection practices. The paper concludes with 10 suggestions to help support practitioners when implementing selection criteria.

Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions.

Unlike conventional antimicrobials, the study of bacterial resistance to silver nanoparticles (AgNPs) remains in its infancy and the mechanism(s) through which it evolves are limited and inconclusive. The central question remains whether bacterial resistance is driven by the AgNPs, released Ag(I) ions or a combination of these and other factors. Here, we show a specific resistance in an Escherichia coli K-12 MG1655 strain to subinhibitory concentrations of AgNPs, and not Ag(I) ions, as indicated by a statistically significant greater-than-twofold increase in the minimum inhibitory concentration occurring after eight repeated passages that was maintained after the AgNPs were removed and reintroduced. Whole-population genome sequencing identified a cusS mutation associated with the heritable resistance that possibly increased silver ion efflux. Finally, we rule out the effect of particle aggregation on resistance and suggest that the mechanism of resistance may be enhanced or mediated by flagellum-based motility.

Talent Research in Sport 1990-2018: A Scoping Review.

Several recent systematic and targeted reviews have highlighted limitations in our understanding of talent in sport. However, a comprehensive profile of where the scientific research has focused would help identify gaps in current knowledge. Our goal in this scoping review was (a) to better understand what others have done in the field of research (e.g., what groups have been examined using what research designs and in what areas), (b) to summarize the constituent areas of research in a meaningful way, (c) to help identify gaps in the research, and (d) to encourage future research to address these gaps. Peer-reviewed articles written in English that met several inclusion criteria were analyzed. A total of 1,899 articles were identified, and the descriptive findings revealed a relatively narrow focus of research on talent in sport. Specifically, the majority of examined articles focused on (a) males only, (b) the sport of soccer, (c) perceptual cognitive variables, (d) developing athletes, (e) adult samples, and (f) cross-sectional designs. For better or worse, the concept of talent remains a central element of how coaches, practitioners, and scientists think about athlete development. Findings from this scoping review highlight the continued need to explore issues related to talent identification, selection, and development in more diverse samples (e.g., female athletes and younger ages) and contexts (e.g., from Africa, Asia, and South America). There is also a clear necessity to focus on under-researched areas using alternative methodologies.

Emerging investigator series: characterization of silver and silver nanoparticle interactions with zinc finger peptides.

In biological systems, chemical and physical transformations of engineered silver nanomaterials (AgENMs) are mediated, in part, by proteins and other biomolecules. Metalloprotein interactions with AgENMs are also central in understanding toxicity and antimicrobial and resistance mechanisms. Despite their readily available thiolate and amine ligands, zinc finger (ZF) peptides have thus far escaped study in reaction with AgENMs and their Ag(I) oxidative dissolution product. We report spectroscopic studies that characterize AgENM and Ag(I) interactions with two ZF peptides that differ in sequence, but not in metal binding ligands: the ZF consensus peptide CP-CCHC and the C-terminal zinc finger domain of HIV-1 nucleocapsid protein p7 (NCp7_C). Both ZF peptides catalyze AgENM (10 and 40 nm, citrate coated) dissolution and agglomeration, two important AgENM transformations that impact bioreactivity. AgENMs and their oxidative dissolution product, Ag(I)(aq), mediate changes to ZF peptide structure and metalation as well. Spectroscopic titrations of Ag(I) into apo-ZF peptides show an Ag(I)-thiolate charge transfer band, indicative of Ag(I)-ZF binding. Fluorescence studies of the Zn(II)-NCp_7 complex indicate that the Ag(I) also effectively competes with the Zn(II) to drive Zn(II) displacement from the ZFs. Upon interaction with AgENMs, Zn(II) bound ZF peptides show a secondary structural change in circular dichroism spectroscopy toward an apo-like structure. The results suggest that Ag(I) and AgENMs may alter ZF protein function within the cell.