ABSTRACT
A reliable, scalable, cost-effective, and chromatography-free synthesis of 4-azido-l-phenylalanine beginning from l-phenylalanine is described. Investigations into the safety of the synthesis reveal that the Ullman-like Cu(I)-catalyzed azidation step does not represent a significant risk. The isolated 4-azido-l-phenylalanine product, however, exhibits previously undocumented explosive characteristics.
Subject(s)
Azides/chemical synthesis , Phenylalanine/analogs & derivatives , Azides/chemistry , Catalysis , Copper/chemistry , Molecular Structure , Phenylalanine/chemical synthesis , Phenylalanine/chemistryABSTRACT
Caveolin-1 is a target for academic and pharmaceutical research due to its many cellular roles and associated diseases. We report peptide WL47 (1), a small, high-affinity, selective disrupter of caveolin-1 oligomers. Developed and optimized through screening and analysis of synthetic peptide libraries, ligand 1 has 7500-fold improved affinity compared to its T20 parent ligand and an 80% decrease in sequence length. Ligand 1 will permit targeted study of caveolin-1 function.
Subject(s)
Biopolymers/metabolism , Caveolin 1/metabolism , LigandsABSTRACT
National parks and bioreserves are key conservation tools used to protect species and their habitats within the confines of fixed political boundaries. This inflexibility may be their "Achilles' heel" as conservation tools in the face of emerging global-scale environmental problems such as climate change. Global climate change, brought about by rising levels of greenhouse gases, threatens to alter the geographic distribution of many habitats and their component species. With these changes comes great uncertainty about the future ability of parks and protected areas to meet their conservation mandates. We report here on an analysis aimed at assessing the extent of mammalian species turnover that may be experienced in eight selected U.S. national parks if climate change causes mammalian species within the continental U.S. to relocate to new geographic locations. Due to species losses of up to 20% and drastic influxes of new species, national parks are not likely to meet their mandate of protecting current biodiversity within park boundaries. This approach represents a conservative prognosis. As species assemblages change, new interactions between species may lead to less predictable indirect effects of climate change, increasing the toll beyond that found in this study.
