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1.
J Comp Neurol ; 530(1): 6-503, 2022 01.
Article in English | MEDLINE | ID: mdl-34525221

ABSTRACT

Increasing interest in studies of prenatal human brain development, particularly using new single-cell genomics and anatomical technologies to create cell atlases, creates a strong need for accurate and detailed anatomical reference atlases. In this study, we present two cellular-resolution digital anatomical atlases for prenatal human brain at postconceptional weeks (PCW) 15 and 21. Both atlases were annotated on sequential Nissl-stained sections covering brain-wide structures on the basis of combined analysis of cytoarchitecture, acetylcholinesterase staining, and an extensive marker gene expression dataset. This high information content dataset allowed reliable and accurate demarcation of developing cortical and subcortical structures and their subdivisions. Furthermore, using the anatomical atlases as a guide, spatial expression of 37 and 5 genes from the brains, respectively, at PCW 15 and 21 was annotated, illustrating reliable marker genes for many developing brain structures. Finally, the present study uncovered several novel developmental features, such as the lack of an outer subventricular zone in the hippocampal formation and entorhinal cortex, and the apparent extension of both cortical (excitatory) and subcortical (inhibitory) progenitors into the prenatal olfactory bulb. These comprehensive atlases provide useful tools for visualization, segmentation, targeting, imaging, and interpretation of brain structures of prenatal human brain, and for guiding and interpreting the next generation of cell census and connectome studies.


Subject(s)
Atlases as Topic , Brain/growth & development , Entorhinal Cortex/growth & development , Hippocampus/growth & development , Animals , Female , Humans , Pregnancy
2.
Nature ; 575(7781): 195-202, 2019 11.
Article in English | MEDLINE | ID: mdl-31666704

ABSTRACT

The mammalian cortex is a laminar structure containing many areas and cell types that are densely interconnected in complex ways, and for which generalizable principles of organization remain mostly unknown. Here we describe a major expansion of the Allen Mouse Brain Connectivity Atlas resource1, involving around a thousand new tracer experiments in the cortex and its main satellite structure, the thalamus. We used Cre driver lines (mice expressing Cre recombinase) to comprehensively and selectively label brain-wide connections by layer and class of projection neuron. Through observations of axon termination patterns, we have derived a set of generalized anatomical rules to describe corticocortical, thalamocortical and corticothalamic projections. We have built a model to assign connection patterns between areas as either feedforward or feedback, and generated testable predictions of hierarchical positions for individual cortical and thalamic areas and for cortical network modules. Our results show that cell-class-specific connections are organized in a shallow hierarchy within the mouse corticothalamic network.


Subject(s)
Cerebral Cortex/anatomy & histology , Cerebral Cortex/cytology , Neural Pathways/anatomy & histology , Neural Pathways/cytology , Thalamus/anatomy & histology , Thalamus/cytology , Animals , Axons/physiology , Cerebral Cortex/physiology , Female , Integrases/genetics , Integrases/metabolism , Male , Mice , Mice, Inbred C57BL , Neural Pathways/physiology , Thalamus/physiology
3.
Nature ; 563(7729): 72-78, 2018 11.
Article in English | MEDLINE | ID: mdl-30382198

ABSTRACT

The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing. Nearly all types of GABA (γ-aminobutyric acid)-containing neurons are shared across both areas, whereas most types of glutamatergic neurons were found in one of the two areas. By combining single-cell RNA sequencing and retrograde labelling, we match transcriptomic types of glutamatergic neurons to their long-range projection specificity. Our study establishes a combined transcriptomic and projectional taxonomy of cortical cell types from functionally distinct areas of the adult mouse cortex.


Subject(s)
Gene Expression Profiling , Neocortex/cytology , Neocortex/metabolism , Animals , Biomarkers/analysis , Female , GABAergic Neurons/metabolism , Glutamic Acid/metabolism , Male , Mice , Motor Cortex/anatomy & histology , Motor Cortex/cytology , Motor Cortex/metabolism , Neocortex/anatomy & histology , Organ Specificity , Sequence Analysis, RNA , Single-Cell Analysis , Visual Cortex/anatomy & histology , Visual Cortex/cytology , Visual Cortex/metabolism
5.
J Comp Neurol ; 524(16): 3127-481, 2016 11 01.
Article in English | MEDLINE | ID: mdl-27418273

ABSTRACT

Detailed anatomical understanding of the human brain is essential for unraveling its functional architecture, yet current reference atlases have major limitations such as lack of whole-brain coverage, relatively low image resolution, and sparse structural annotation. We present the first digital human brain atlas to incorporate neuroimaging, high-resolution histology, and chemoarchitecture across a complete adult female brain, consisting of magnetic resonance imaging (MRI), diffusion-weighted imaging (DWI), and 1,356 large-format cellular resolution (1 µm/pixel) Nissl and immunohistochemistry anatomical plates. The atlas is comprehensively annotated for 862 structures, including 117 white matter tracts and several novel cyto- and chemoarchitecturally defined structures, and these annotations were transferred onto the matching MRI dataset. Neocortical delineations were done for sulci, gyri, and modified Brodmann areas to link macroscopic anatomical and microscopic cytoarchitectural parcellations. Correlated neuroimaging and histological structural delineation allowed fine feature identification in MRI data and subsequent structural identification in MRI data from other brains. This interactive online digital atlas is integrated with existing Allen Institute for Brain Science gene expression atlases and is publicly accessible as a resource for the neuroscience community. J. Comp. Neurol. 524:3127-3481, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.


Subject(s)
Anatomy, Artistic , Brain/anatomy & histology , Adult , Brain/diagnostic imaging , Brain/metabolism , Diffusion Magnetic Resonance Imaging , Female , Humans , Image Processing, Computer-Assisted , Magnetic Resonance Imaging , Neurofilament Proteins/metabolism , Parvalbumins/metabolism
6.
Cereb Cortex ; 25(2): 433-49, 2015 Feb.
Article in English | MEDLINE | ID: mdl-24014670

ABSTRACT

The neocortex contains diverse populations of excitatory neurons segregated by layer and further definable by their specific cortical and subcortical projection targets. The current study describes a systematic approach to identify molecular correlates of specific projection neuron classes in mouse primary somatosensory cortex (S1), using a combination of in situ hybridization (ISH) data mining, marker gene colocalization, and combined retrograde labeling with ISH for layer-specific marker genes. First, we identified a large set of genes with specificity for each cortical layer, and that display heterogeneous patterns within those layers. Using these genes as markers, we find extensive evidence for the covariation of gene expression and projection target specificity in layer 2/3, 5, and 6, with individual genes labeling neurons projecting to specific subsets of target structures. The combination of gene expression and target specificity imply a great diversity of projection neuron classes that is similar to or greater than that of GABAergic interneurons. The covariance of these 2 phenotypic modalities suggests that these classes are both discrete and genetically specified.


Subject(s)
Neurons/cytology , Neurons/physiology , Somatosensory Cortex/cytology , Somatosensory Cortex/physiology , Animals , Atlases as Topic , Data Mining , Gene Expression/physiology , In Situ Hybridization, Fluorescence , Male , Mice , Mice, Inbred C57BL , Neural Pathways/cytology , Neural Pathways/physiology , Neuronal Tract-Tracers
7.
J Manipulative Physiol Ther ; 37(7): 476-84, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25113654

ABSTRACT

OBJECTIVE: The purpose of this study was to investigate the presence of intraarticular gas bubbles in the trapeziometacarpal joint cavity after chiropractic manipulation with audible cavitation and to assess the state of the gas bubbles after a 20-minute refractory period. METHODS: This investigation included 18 asymptomatic male and female participants between the ages of 21 and 26 years. High-resolution (15 MHz) sonograms of the trapeziometacarpal articular cavity were obtained by an experienced musculoskeletal ultrasonographer at 3 intervals: premanipulation, within 30 seconds postmanipulation, and at 20 minutes postmanipulation. The sonograms were saved as digital copies for subsequent reports that were correlated with reports compiled during dynamic visualization of the articular cavity. Data were extracted from the reports for analysis. RESULTS: The premanipulative sonograms showed that 27.78% of joints contained minute gas bubbles, also known as microcavities, within the synovial fluid before the joint was manipulated. The remaining 72.22% of joints contained no intraarticular microcavities. All of the postmanipulative sonograms revealed numerous large conspicuous gas bubbles within the synovial fluid. The postrefractory sonograms showed that, in 66.66% of the synovial fluid, gas bubbles were still visible, whereas the remaining 33.34% had no presence of gas bubbles or microcavities, and the synovial fluid had returned to its premanipulative state. CONCLUSION: The findings of this study suggest that synovial fluid may contain intraarticular microcavities even before a manipulation is performed. Numerous large intraarticular gas bubbles are formed during manipulation due to cavitation of the synovial fluid and were observed in the absence of an axial distractive load at the time of imaging. In most cases, these gas bubbles remained within the joint for longer than 20 minutes.


Subject(s)
Gases , Hand Joints/diagnostic imaging , Manipulation, Chiropractic , Metacarpal Bones/diagnostic imaging , Trapezium Bone/diagnostic imaging , Adult , Female , Humans , Male , Ultrasonography , Young Adult
8.
Neuron ; 83(2): 309-323, 2014 Jul 16.
Article in English | MEDLINE | ID: mdl-24952961

ABSTRACT

To provide a temporal framework for the genoarchitecture of brain development, we generated in situ hybridization data for embryonic and postnatal mouse brain at seven developmental stages for ∼2,100 genes, which were processed with an automated informatics pipeline and manually annotated. This resource comprises 434,946 images, seven reference atlases, an ontogenetic ontology, and tools to explore coexpression of genes across neurodevelopment. Gene sets coinciding with developmental phenomena were identified. A temporal shift in the principles governing the molecular organization of the brain was detected, with transient neuromeric, plate-based organization of the brain present at E11.5 and E13.5. Finally, these data provided a transcription factor code that discriminates brain structures and identifies the developmental age of a tissue, providing a foundation for eventual genetic manipulation or tracking of specific brain structures over development. The resource is available as the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org).


Subject(s)
Brain Mapping/methods , Brain/growth & development , Gene Expression Profiling/methods , Gene Expression Regulation, Developmental , Animals , Gene Expression , Mice
9.
Nature ; 508(7495): 207-14, 2014 Apr 10.
Article in English | MEDLINE | ID: mdl-24695228

ABSTRACT

Comprehensive knowledge of the brain's wiring diagram is fundamental for understanding how the nervous system processes information at both local and global scales. However, with the singular exception of the C. elegans microscale connectome, there are no complete connectivity data sets in other species. Here we report a brain-wide, cellular-level, mesoscale connectome for the mouse. The Allen Mouse Brain Connectivity Atlas uses enhanced green fluorescent protein (EGFP)-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain. This systematic and standardized approach allows spatial registration of individual experiments into a common three dimensional (3D) reference space, resulting in a whole-brain connectivity matrix. A computational model yields insights into connectional strength distribution, symmetry and other network properties. Virtual tractography illustrates 3D topography among interconnected regions. Cortico-thalamic pathway analysis demonstrates segregation and integration of parallel pathways. The Allen Mouse Brain Connectivity Atlas is a freely available, foundational resource for structural and functional investigations into the neural circuits that support behavioural and cognitive processes in health and disease.


Subject(s)
Brain/anatomy & histology , Brain/cytology , Connectome , Animals , Atlases as Topic , Axons/physiology , Cerebral Cortex/cytology , Corpus Striatum/cytology , Male , Mice , Mice, Inbred C57BL , Models, Neurological , Neuroanatomical Tract-Tracing Techniques , Thalamus/cytology
10.
J Comp Neurol ; 522(9): 1989-2012, 2014 Jun 15.
Article in English | MEDLINE | ID: mdl-24639291

ABSTRACT

As an anterograde neuronal tracer, recombinant adeno-associated virus (AAV) has distinct advantages over the widely used biotinylated dextran amine (BDA). However, the sensitivity and selectivity of AAV remain uncharacterized for many brain regions and species. To validate this tracing method further, AAV (serotype 1) was systematically compared with BDA as an anterograde tracer by injecting both tracers into three cortical and 15 subcortical regions in C57BL/6J mice. Identical parameters were used for our sequential iontophoretic injections, producing injections of AAV that were more robust in size and in density of neurons infected compared with those of BDA. However, these differences did not preclude further comparison between the tracers, because the pairs of injections were suitably colocalized and contained some percentage of double-labeled neurons. A qualitative analysis of projection patterns showed that the two tracers behave very similarly when injection sites are well matched. Additionally, a quantitative analysis of relative projection intensity for cases targeting primary motor cortex (MOp), primary somatosensory cortex (SSp), and caudoputamen (CP) showed strong agreement in the ranked order of projection intensities between the two tracers. A detailed analysis of the projections of two brain regions (SSp and MOp) revealed many targets that have not previously been described in the mouse or rat. Minor retrograde labeling of neurons was observed in all cases examined, for both AAV and BDA. Our results show that AAV has actions equivalent to those of BDA as an anterograde tracer and is suitable for analysis of neural circuitry throughout the mouse brain.


Subject(s)
Biotin/analogs & derivatives , Brain/anatomy & histology , Dependovirus , Dextrans , Fluorescent Dyes , Neuroanatomical Tract-Tracing Techniques , Neuronal Tract-Tracers , Animals , Cell Count , Immunohistochemistry , Mice, Inbred C57BL , Microscopy, Confocal , Microscopy, Fluorescence , Neural Pathways/anatomy & histology , Neurons/cytology , Photomicrography , Sensitivity and Specificity
11.
Development ; 140(22): 4633-44, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24154525

ABSTRACT

The neurogenic potential of the subgranular zone (SGZ) of the hippocampal dentate gyrus is likely to be regulated by molecular cues arising from its complex heterogeneous cellular environment. Through transcriptome analysis using laser microdissection coupled with DNA microarrays, in combination with analysis of genome-wide in situ hybridization data, we identified 363 genes selectively enriched in adult mouse SGZ. These genes reflect expression in the different constituent cell types, including progenitor and dividing cells, immature granule cells, astrocytes, oligodendrocytes and GABAergic interneurons. Similar transcriptional profiling in the rhesus monkey dentate gyrus across postnatal development identified a highly overlapping set of SGZ-enriched genes, which can be divided based on temporal profiles to reflect maturation of glia versus granule neurons. Furthermore, we identified a neurogenesis-related gene network with decreasing postnatal expression that is highly correlated with the declining number of proliferating cells in dentate gyrus over postnatal development. Many of the genes in this network showed similar postnatal downregulation in mouse, suggesting a conservation of molecular mechanisms underlying developmental and adult neurogenesis in rodents and primates. Conditional deletion of Sox4 and Sox11, encoding two neurogenesis-related transcription factors central in this network, produces a mouse with no hippocampus, confirming the crucial role for these genes in regulating hippocampal neurogenesis.


Subject(s)
Gene Expression Profiling , Hippocampus/metabolism , Macaca mulatta/genetics , Neurogenesis/genetics , Animals , Animals, Newborn , Biomarkers/metabolism , Gene Expression Regulation, Developmental , Gene Regulatory Networks , Genome/genetics , Hippocampus/cytology , Interneurons/cytology , Interneurons/metabolism , Male , Mice , Mice, Inbred C57BL , Multigene Family , Oligodendroglia/cytology , Oligodendroglia/metabolism , SOXC Transcription Factors/genetics , SOXC Transcription Factors/metabolism , Spatio-Temporal Analysis , Transcription, Genetic
12.
Trends Neurosci ; 35(12): 711-4, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23041053

ABSTRACT

The Allen Human Brain Atlas is a freely available multimodal atlas of gene expression and anatomy comprising a comprehensive 'all genes-all structures' array-based dataset of gene expression and complementary in situ hybridization (ISH) gene expression studies targeting selected genes in specific brain regions. Available via the Allen Brain Atlas data portal (www.brain-map.org), the Atlas integrates structure, function, and gene expression data to accelerate basic and clinical research of the human brain in normal and disease states.


Subject(s)
Anatomy, Artistic , Atlases as Topic , Brain/anatomy & histology , Chromosome Mapping , Gene Expression Profiling , Humans , In Situ Hybridization
13.
Cell ; 149(2): 483-96, 2012 Apr 13.
Article in English | MEDLINE | ID: mdl-22500809

ABSTRACT

Although there have been major advances in elucidating the functional biology of the human brain, relatively little is known of its cellular and molecular organization. Here we report a large-scale characterization of the expression of ∼1,000 genes important for neural functions by in situ hybridization at a cellular resolution in visual and temporal cortices of adult human brains. These data reveal diverse gene expression patterns and remarkable conservation of each individual gene's expression among individuals (95%), cortical areas (84%), and between human and mouse (79%). A small but substantial number of genes (21%) exhibited species-differential expression. Distinct molecular signatures, comprised of genes both common between species and unique to each, were identified for each major cortical cell type. The data suggest that gene expression profile changes may contribute to differential cortical function across species, and in particular, a shift from corticosubcortical to more predominant corticocortical communications in the human brain.


Subject(s)
Gene Expression Profiling , Neocortex/metabolism , Temporal Lobe/metabolism , Visual Cortex/metabolism , Adult , Animals , Gene Expression Regulation , Humans , Mice , Neocortex/cytology , Neurons/metabolism , Species Specificity , Temporal Lobe/cytology , Visual Cortex/cytology
14.
Nat Neurosci ; 15(5): 793-802, 2012 Mar 25.
Article in English | MEDLINE | ID: mdl-22446880

ABSTRACT

Cell type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of four knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0 and archaerhodopsin Arch-ER2. All four transgenes mediated Cre-dependent, robust activation or silencing of cortical pyramidal neurons in vitro and in vivo upon light stimulation, with ChR2-EYFP and Arch-ER2 demonstrating light sensitivity approaching that of in utero or virally transduced neurons. We further show specific photoactivation of parvalbumin-positive interneurons in behaving ChR2-EYFP reporter mice. The robust, consistent and inducible nature of our ChR2 mice represents a significant advance over previous lines, and the Arch-ER2 and eNpHR3.0 mice are to our knowledge the first demonstration of successful conditional transgenic optogenetic silencing. When combined with the hundreds of available Cre driver lines, this optimized toolbox of reporter mice will enable widespread investigations of neural circuit function with unprecedented reliability and accuracy.


Subject(s)
Brain/cytology , Integrases/metabolism , Light , Neurogenesis/physiology , Neurons/physiology , Action Potentials/physiology , Animals , Archaeal Proteins , Channelrhodopsins , Electroporation/methods , Halorhodopsins/genetics , Halorhodopsins/metabolism , In Vitro Techniques , Integrases/genetics , Luminescent Proteins/genetics , Mice , Mice, Transgenic , Neurogenesis/genetics , Optics and Photonics , Proteins/genetics , RNA, Untranslated , Wakefulness
15.
Neuron ; 73(6): 1083-99, 2012 Mar 22.
Article in English | MEDLINE | ID: mdl-22445337

ABSTRACT

Genome-wide transcriptional profiling was used to characterize the molecular underpinnings of neocortical organization in rhesus macaque, including cortical areal specialization and laminar cell-type diversity. Microarray analysis of individual cortical layers across sensorimotor and association cortices identified robust and specific molecular signatures for individual cortical layers and areas, prominently involving genes associated with specialized neuronal function. Overall, transcriptome-based relationships were related to spatial proximity, being strongest between neighboring cortical areas and between proximal layers. Primary visual cortex (V1) displayed the most distinctive gene expression compared to other cortical regions in rhesus and human, both in the specialized layer 4 as well as other layers. Laminar patterns were more similar between macaque and human compared to mouse, as was the unique V1 profile that was not observed in mouse. These data provide a unique resource detailing neocortical transcription patterns in a nonhuman primate with great similarity in gene expression to human.


Subject(s)
Macaca mulatta/anatomy & histology , Neocortex/cytology , Neocortex/metabolism , Transcriptome/physiology , Analysis of Variance , Animals , Female , Humans , Male , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mice , Microarray Analysis , Microdissection , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Neural Pathways/physiology , Neurons , Principal Component Analysis , Transcription Factors/genetics , Transcription Factors/metabolism
16.
PLoS One ; 6(9): e24538, 2011.
Article in English | MEDLINE | ID: mdl-21931744

ABSTRACT

Radial glia (RG) are primarily embryonic neuroglial progenitors that express Brain Lipid Binding Protein (Blbp a.k.a. Fabp7) and Glial Fibrillary Acidic Protein (Gfap). We used these transcripts to demarcate the distribution of spinal cord radial glia (SCRG) and screen for SCRG gene expression in the Allen Spinal Cord Atlas (ASCA). We reveal that neonatal and adult SCRG are anchored in a non-ventricular niche at the spinal cord (SC) pial boundary, and express a "signature" subset of 122 genes, many of which are shared with "classic" neural stem cells (NSCs) of the subventricular zone (SVZ) and SC central canal (CC). A core expressed gene set shared between SCRG and progenitors of the SVZ and CC is particularly enriched in genes associated with human disease. Visualizing SCRG in a Fabp7-EGFP reporter mouse reveals an extensive population of SCRG that extend processes around the SC boundary and inwardly (through) the SC white matter (WM), whose abundance increases in a gradient from cervical to lumbar SC. Confocal analysis of multiple NSC-enriched proteins reveals that postnatal SCRG are a discrete and heterogeneous potential progenitor population that become activated by multiple SC lesions, and that CC progenitors are also more heterogeneous than previously appreciated. Gene ontology analysis highlights potentially unique regulatory pathways that may be further manipulated in SCRG to enhance repair in the context of injury and SC disease.


Subject(s)
Gene Expression Regulation, Developmental , Neuroglia/pathology , Spinal Cord/pathology , Stem Cells/cytology , Animals , Autoimmune Diseases/pathology , Encephalomyelitis/metabolism , Fatty Acid-Binding Protein 7 , Fatty Acid-Binding Proteins/metabolism , Gene Expression Profiling , Glial Fibrillary Acidic Protein/biosynthesis , Green Fluorescent Proteins/metabolism , Male , Mice , Mice, Inbred C57BL , Nerve Tissue Proteins/metabolism , Neuroglia/cytology , Neuroglia/metabolism , Phenotype , Spinal Cord/cytology , Spinal Cord Injuries/pathology
17.
J Comp Neurol ; 519(11): 2061-89, 2011 Aug 01.
Article in English | MEDLINE | ID: mdl-21491433

ABSTRACT

The disrupted cortical lamination phenotype in reeler mice and subsequent identification of the Reelin signaling pathway have strongly informed models of cortical development. We describe here a marker-based phenotyping approach to reexamine the cytoarchitectural consequences of Reelin deficiency, using high-throughput histology and newly identified panels of highly specific molecular markers. The resulting cell-type-level cytoarchitectural analysis revealed novel features of abnormal patterning in the male reeler mouse not obvious with less specific markers or histology. The reeler cortex has been described as a rough laminar inversion, but the data presented here are not compatible with this model. The reeler cortex is disrupted in a more complex fashion, with some regions showing a mirror-image laminar phenotype. Major rostrocaudal and cell-type-specific differences in the laminar phenotype between cortical areas are detailed. These and similar findings in hippocampus and amygdala have implications for mechanisms of normal brain development and abnormalities in neurodevelopmental disorders.


Subject(s)
Amygdala/cytology , Cell Adhesion Molecules, Neuronal/deficiency , Extracellular Matrix Proteins/deficiency , Hippocampus/cytology , Neocortex/cytology , Nerve Tissue Proteins/deficiency , Serine Endopeptidases/deficiency , Amygdala/abnormalities , Amygdala/growth & development , Amygdala/metabolism , Animals , Biomarkers/metabolism , Cell Adhesion Molecules, Neuronal/genetics , Extracellular Matrix Proteins/genetics , Hippocampus/abnormalities , Hippocampus/growth & development , Hippocampus/metabolism , In Situ Hybridization , Male , Mice , Mice, Neurologic Mutants , Neocortex/abnormalities , Neocortex/growth & development , Neocortex/metabolism , Nerve Tissue Proteins/genetics , Phenotype , Reelin Protein , Serine Endopeptidases/genetics , Signal Transduction/physiology
18.
Front Syst Neurosci ; 4: 162, 2011.
Article in English | MEDLINE | ID: mdl-21283555

ABSTRACT

The putative excitatory and inhibitory cell classes within the mouse primary visual cortex V1 have different functional properties as studied using recording microelectrode. Excitatory neurons show high selectivity for the orientation angle of moving gratings while the putative inhibitory neurons show poor selectivity. However, the study of selectivity of the genetically identified interneurons and their subtypes remain controversial. Here we use novel Cre-driver and reporter mice to identify genetic subpopulations in vivo for two-photon calcium dye imaging: Wfs1(+)/Gad1(-) mice that labels layer 2/3 excitatory cell population and Pvalb(+)/Gad1(+) mice that labels a genetic subpopulation of inhibitory neurons. The cells in both mice were identically labeled with a tdTomato protein, visible in vivo, using a Cre-reporter line. We found that the Wfs1(+) cells exhibited visual tuning properties comparable to the excitatory population, i.e., high selectivity and tuning to the angle, direction, and spatial frequency of oriented moving gratings. The functional tuning of Pvalb(+) neurons was consistent with previously reported narrow-spiking interneurons in microelectrode studies, exhibiting poorer selectivity than the excitatory neurons. This study demonstrates the utility of Cre-transgenic mouse technology in selective targeting of subpopulations of neurons and makes them amenable to structural, functional, and connectivity studies.

19.
Front Neurosci ; 4: 165, 2010.
Article in English | MEDLINE | ID: mdl-21088695

ABSTRACT

Sleep deprivation (SD) leads to a suite of cognitive and behavioral impairments, and yet the molecular consequences of SD in the brain are poorly understood. Using a systematic immediate-early gene (IEG) mapping to detect neuronal activation, the consequences of SD were mapped primarily to forebrain regions. SD was found to both induce and suppress IEG expression (and thus neuronal activity) in subregions of neocortex, striatum, and other brain regions. Laser microdissection and cDNA microarrays were used to identify the molecular consequences of SD in seven brain regions. In situ hybridization (ISH) for 222 genes selected from the microarray data and other sources confirmed that robust molecular changes were largely restricted to the forebrain. Analysis of the ISH data for 222 genes (publicly accessible at http://sleep.alleninstitute.org) provided a molecular and anatomic signature of the effects of SD on the brain. The suprachiasmatic nucleus (SCN) and the neocortex exhibited differential regulation of the same genes, such that in the SCN genes exhibited time-of-day effects while in the neocortex, genes exhibited only SD and waking (W) effects. In the neocortex, SD activated gene expression in areal-, layer-, and cell type-specific manner. In the forebrain, SD preferentially activated excitatory neurons, as demonstrated by double-labeling, except for striatum which consists primarily of inhibitory neurons. These data provide a characterization of the anatomical and cell type-specific signatures of SD on neuronal activity and gene expression that may account for the associated cognitive and behavioral effects.

20.
Proc Natl Acad Sci U S A ; 107(44): 19049-54, 2010 Nov 02.
Article in English | MEDLINE | ID: mdl-20956311

ABSTRACT

Considerable progress has been made in understanding variations in gene sequence and expression level associated with phenotype, yet how genetic diversity translates into complex phenotypic differences remains poorly understood. Here, we examine the relationship between genetic background and spatial patterns of gene expression across seven strains of mice, providing the most extensive cellular-resolution comparative analysis of gene expression in the mammalian brain to date. Using comprehensive brainwide anatomic coverage (more than 200 brain regions), we applied in situ hybridization to analyze the spatial expression patterns of 49 genes encoding well-known pharmaceutical drug targets. Remarkably, over 50% of the genes examined showed interstrain expression variation. In addition, the variability was nonuniformly distributed across strain and neuroanatomic region, suggesting certain organizing principles. First, the degree of expression variance among strains mirrors genealogic relationships. Second, expression pattern differences were concentrated in higher-order brain regions such as the cortex and hippocampus. Divergence in gene expression patterns across the brain could contribute significantly to variations in behavior and responses to neuroactive drugs in laboratory mouse strains and may help to explain individual differences in human responsiveness to neuroactive drugs.


Subject(s)
Brain/metabolism , Gene Expression Regulation/physiology , Animals , Brain/cytology , Gene Expression Profiling , Gene Expression Regulation/drug effects , Humans , In Situ Hybridization , Mice , Species Specificity
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