The effect of anthropogenic substrate-borne vibrations on locomotion of the fiddler crab Austruca lactea.

The anthropogenic construction activities on the coasts, such as pile-driving, generate vibrations that propagate through the substrate. Such substrate-borne vibrations could potentially affect marine organisms inhabiting the benthic environments. However, there is a lack of documented studies on the effects of vibrations on benthic animals. To investigate whether anthropogenic substrate-borne vibrations such as pile-driving operation influence the fiddler crab, Austruca lactea, we measured their locomotion response under vibrations of 35, 120, 250, 500, and 750 Hz generated by a vibrator. We compared the locomotion of crabs between control and vibration-treatment groups using videography. The duration of movements was significantly lower under 120 Hz vibrations compared to the control. Moreover, crab velocity was significantly higher under vibrations of 120 Hz and 250 Hz compared to the control group. Our result suggests that A. lactea can detect low-frequency substrate-borne vibrations and experience stress, leading to increased energy consumption.

Archival records of the Antarctic clam shells from Marian Cove, King George Island suggest a protective mechanism against ocean acidification.

Continuous emissions of anthropogenic CO2 are changing the atmospheric and oceanic environment. Although some species may have compensatory mechanisms to acclimatize or adapt to the changing environment, most marine organisms are negatively influenced by climate change. In this study, we aimed to understand the compensatory mechanisms of the Antarctic clam, Laternula elliptica, to climate-related stressors by using archived shells from 1995 to 2018. Principal component analysis revealed that seawater pCO2 and salinity in the Antarctic Ocean, which have increased since the 2000's, are the most influential factors on the characteristics of the shell. The periostracum thickness ratio and nitrogen on the outermost surface have increased, and the dissolution area (%) has decreased. Furthermore, the calcium content and mechanical properties of the shells have not changed. The results suggest that L. elliptica retains the mechanism of protecting the shell from high pCO2 by thickening the periostracum as a phenotype plasticity.

Reduction-Responsive Chitosan-Based Injectable Hydrogels for Enhanced Anticancer Therapy.

Selective delivery of anticancer drug molecules to the tumor site enhances local drug dosages, which leads to the death of cancer cells while simultaneously minimizing the negative effects of chemotherapy on other tissues, thereby improving the patient's quality of life. To address this need, we developed reduction-responsive chitosan-based injectable hydrogels via the inverse electron demand Diels-Alder reaction between tetrazine groups of disulfide-based cross-linkers and norbornene groups of chitosan derivatives, which were applied to the controlled delivery of doxorubicin (DOX). The swelling ratio, gelation time (90-500 s), mechanical strength (G'~350-850 Pa), network morphology, and drug-loading efficiency (≥92%) of developed hydrogels were investigated. The in vitro release studies of the DOX-loaded hydrogels were performed at pH 7.4 and 5.0 with and without DTT (10 mM). The biocompatibility of pure hydrogel and the in vitro anticancer activity of DOX-loaded hydrogels were demonstrated via MTT assay on HEK-293 and HT-29 cancer cell lines, respectively.

The effect of molecular weight and chemical structure of cross-linkers on the properties of redox-responsive hyaluronic acid hydrogels.

In this work, we investigated the effect of the size and the chemical structure of crosslinkers on the properties of hyaluronic acid-based hydrogels prepared via an inverse electron demand Diels-Alder reaction. Hydrogels having loose and dense networks were designed by cross-linkers with and without polyethylene glycol (PEG) spacers of different molecular weights (1000 and 4000 g/mol). The study showed that the properties of hydrogels such as swelling ratios (20-55 times), morphology, stability, mechanical strength (storage modulus in the range 175-858 Pa), and drug loading efficiency (87 % ~ 90 %) were greatly influenced by the addition of PEG and changing its molecular weight in the cross-linker. Particularly, the presence of PEG chains in redox- responsive crosslinkers increased the doxorubicin release (85 %, after 168 h) and the degradation rate (96 %, after 10 d) of hydrogels in the simulated reducing medium (10 mM DTT). The in vitro cytotoxicity experiments conducted for HEK-293 cells revealed that the formulated hydrogels were biocompatible, which could be a promising candidate for drug delivery applications.

Microplastics in large marine animals stranded in the Republic of Korea.

Microplastics (MPs) are found in every ocean and are frequently ingested by marine animals. This study analyzed MPs in the stomachs and intestines of 12 large marine animals comprising one fin whale (Balaenoptera physalus), seven finless porpoises (Neophocaena asiaeorientalis), two loggerhead turtles (Caretta caretta), one Indo-Pacific bottlenose dolphin (Tursiops aduncus), and one common dolphin (Delphinus delphis) that were stranded off the Republic of Korea between 2019 and 2021. MPs were detected with a mean abundance of 3.42 ± 3.2 items/g and were predominantly of transparent-white, fragment-shaped polypropylene smaller than 200 µm. The abundance of MPs found did not correlate with the biological information (maturity, body length) of the finless porpoises and there were no significant differences in the abundance of MPs between the stomachs and intestines. These results cannot accurately assess the impact of MPs on large marine animals, so further studies are necessary to understand how MPs can potentially affect them.

NIR-degradable and biocompatible hydrogels derived from hyaluronic acid and coumarin for drug delivery and bio-imaging.

In this work, bioorthogonal and photodegradable hydrogels derived from norbornene (Nb) functionalized hyaluronic acid and a water soluble coumarin-based cross-linker possessing terminal tetrazine (Tz) groups, were developed for NIR-responsive release of doxorubicin (DOX). The inverse electron demand Diels-Alder cross-linking reaction between Nb and Tz functionalities formed the hydrogels at physiological conditions, whereas N2 gas liberated during the reaction created pores in the hydrogels. The gelation time ranges (about 5-20 min) and the viscoelastic behavior (G' ~ 346-1380 Pa) demonstrated that the resulting hydrogels were injectable and possessed tunable mechanical properties. Moreover, hydrogels released the encapsulated DOX upon NIR irradiation, owing to the NIR-responsive cleavage of coumarin-ester, and consequently, induced anti-tumor activity in BT-20 cancer cells. Additionally, the hydrogels could be excited at various wavelengths of the visible spectrum and can emit green to red fluorescence, demonstrating their simultaneous photo-responsive drug release and bio-imaging applications.

Possible link between derelict fishing gear and sea turtle strandings in coastal areas.

Ghost fishing via a derelict fishing gear (DFG) is a critical threat to marine organisms. To explore the effect of DFG on sea turtle strandings, the DFG distribution was compared at two sites on Jeju Island (South Korea) with a contrasting number of strandings. Coastal areas in northern Jeju Island were surveyed during dives with scuba equipment, and the DFG from two sites, Gwideok-ri and Sinchang-ri was collected and compared in terms of quantity and size of the items. Fishing line was more common, longer, and thicker in Gwideok-ri than in Sinchang-ri, while other types of DFG did not differ between the two sites. In addition, necropsies on two loggerhead sea turtles discovered on Jeju Island found fishing lines with fishing hooks in the oral cavity of both carcasses. This suggests that derelict recreational fishing lines may pose a significant threat to sea turtles in coastal areas.

Fast Absorbent and Highly Bioorthogonal Hydrogels Developed by IEDDA Click Reaction for Drug Delivery Application.

In this work, we engineered highly biocompatible and fast absorbent injectable hydrogels derived from norbornene (Nb)-functionalized hyaluronic acid (HA-Nb) and a water-soluble cross-linker possessing tetrazine (Tz) functional groups on both ends of polyethylene glycol (PEG-DTz). The by-product (nitrogen gas) of the inverse electron demand Diels−Alder (IEDDA) cross-linking reaction carved porosity in the resulting hydrogels. By varying the molar ratio of HA-Nb and PEG-DTz (Nb:Tz = 10:10, 10:5, 10:2.5), we were able to formulate hydrogels with tunable porosity, gelation time, mechanical strength, and swelling ratios. The hydrogels formed quickly (gelation time < 100 s), offering a possibility to use them as an injectable drug delivery system. The experimental data showed rapid swelling and a high swelling ratio thanks to the existence of PEG chains and highly porous architectures of the hydrogels. The hydrogels were able to encapsulate a high amount of curcumin (~99%) and released the encapsulated curcumin in a temporal pattern. The PEG-DTz cross-linker, HA-Nb, and the resulting hydrogels showed no cytotoxicity in HEK-293 cells. These fast absorbent hydrogels with excellent biocompatibility fabricated from HA-Nb and the IEDDA click-able cross-linker could be promising drug carriers for injectable drug delivery applications.

First record of plastic debris ingestion by a fin whale (Balaenoptera physalus) in the sea off East Asia.

Marine pollution caused by plastic litter can threaten the survival and health of marine organisms. In 2019, a juvenile fin whale (Balaenoptera physalus, length: 13.02 m, weight 12,000 kg) was found dead floating on the sea near Jeju Island, Republic of Korea. During the dissection, 45 plastic particles were found in the body of the whale, including fishing lines, plastic filaments, pieces of fishing nets, and Styrofoam particles. The largest item found was a piece of fishing line (1180 mm in length and 1.15 mm in thickness). Filaments, both bundled and separated, were more frequent. Some of the filaments found were entangled with the baleen plate bristles. These observations suggest that plastic pollution is a potential risk for baleen whale species. This is the first record of plastic ingestion by a vulnerable baleen whale species in the sea off East Asia.