Studies on antifungal properties of phenol complexes with secondary amines. 2. Antifungal properties in vitro of the complex of 2,4,5-trichlorophenol with dicyclohexylamine.

In studies in vitro it has been found that 2,4,5-trichlorophenol in the form of a complex with dicyclohexylamine is two times more active an antifungal agent than free chlorophenol.

Phospholipids and cholesterol in the liver mitochondrial fraction in mice with transplantable leukemia P 388.

BDF1 hybrid mice, were given 10(4) lymphatic leukemia cells P 388 intraperitoneally. They were killed on 5th day and 11th day of the experiment. In the plasma the level of cholesterol was determined. Inner organs, i.e. liver, spleen, lymph nodes and thymus were verified histopathologically. Mitochondrial fraction lipids were extracted from liver and separated by thin layer chromatography. It was proved that the amount of mitochondrial fraction lipids of leukemic mice was considerably higher than in healthy mice and it increased together with the process of the neoplasma. In the group of leukemic animals the level of phosphatidylcholine, phosphatidylethanolamine, cholesterol and sphingomyelin increased in comparison with healthy mice. The results concerning phospholipids and cholesterol level in leukemic animals were interpreted in the aspect of the interaction of these compounds with vitamin E, polyunsaturated fatty acids and free radicals.

[Content of the heavy metals, Zn, Cu, Cd and Pb in the organs of lympholeukemic cows]. / Uber den Gehalt der Schwermetalle Zn, Cu, Cd, Pb in den Organen lympholeukotischer Hühe.

Zn, Cu, Cd and Pb contents in liver, spleen and lymph nodes of 30 cows with lymphatic leukemia were determined using polarographic method. In leukemic tissues more or less prominent increase of mentioned metals was observed. Results of the examinations would indicate a partical disappearance of natural antagonism between particular elements in neoplastic tissues. Metals accumulation in leukemic tissue is prabably a secondary phenomenon in relation to virus infection. Alterated by virus physicochemical cell properties promote not only accumulation bat also perversion of heavy metals to tissues.