Subject(s)
Community-Acquired Infections/microbiology , Methicillin Resistance , Soft Tissue Infections/microbiology , Staphylococcal Skin Infections/microbiology , Staphylococcus aureus/classification , Staphylococcus aureus/drug effects , Adolescent , Adult , Aged , Aged, 80 and over , Argentina , Child , Female , Humans , Male , Middle Aged , Staphylococcus aureus/isolation & purification , Virulence Factors/geneticsABSTRACT
A rapid modified spot CAMP test using 183 clinical isolates of beta haemolytic streptococci was compared with the standard CAMP test described by Christie et al. The scheme of biochemical identification and serological confirmation was taken as reference method. The sensitivity of both tests was 100%, and the specificity of the rapid and standard tests was 96.8% and 88.9% respectively. The modified spot CAMP test is a rapid, inexpensive and accurate method for the identification of group B streptococci, and is more specific than the standard CAMP test.
Subject(s)
Bacterial Proteins/analysis , Bacterial Typing Techniques/methods , Streptococcus agalactiae/isolation & purification , Agar , Animals , Hemolysin Proteins , Hemolysis , Reproducibility of Results , Sensitivity and Specificity , Sheep/bloodABSTRACT
Microbiologic study of bacteremia and fungemia in chronic hemodialysis patients. Bloodstream infections are the second cause of death in patients in chronic hemodialysis (CHD), and the knowledge of the epidemiology is useful to establish proper empiric therapies. The aim of this study was to evaluate the frequency and distribution of microorganisms, in bacteremia and fungemia in 530 patients in CHD. Two hundred and forty eight blood culture series from 114 patients with suspected bacteremia were processed; 44% of them were positive from which 71% (n=78) were clinically significative and belonged to 58 patients. Sixty eight percent of these isolates were gram-positive cocci (n:53), and 22% gram-negative rods (n:17). Staphylococcus aureus was the most prevalent pathogen showing 23% of methicillin-resistance. Candida spp. was the fourth pathogen most common in frequency.
Subject(s)
Bacteremia/microbiology , Fungemia/microbiology , Renal Dialysis , Argentina/epidemiology , Bacteremia/complications , Bacteremia/epidemiology , Candida/isolation & purification , Candidiasis/complications , Candidiasis/epidemiology , Candidiasis/microbiology , Disease Susceptibility , Drug Resistance, Multiple, Bacterial , Drug Resistance, Multiple, Fungal , Fungemia/complications , Fungemia/epidemiology , Gram-Negative Bacteria/drug effects , Gram-Negative Bacterial Infections/complications , Gram-Negative Bacterial Infections/epidemiology , Gram-Negative Bacterial Infections/microbiology , Gram-Positive Bacterial Infections/complications , Gram-Positive Bacterial Infections/epidemiology , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Cocci/drug effects , Gram-Positive Cocci/isolation & purification , Humans , Kidney Failure, Chronic/complications , Kidney Failure, Chronic/therapyABSTRACT
A rapid modified spot CAMP test using 183 clinical isolates of β haemolytic streptococci was compared with the standard CAMP test described by Christie et al. The scheme of biochemical identification and serological confirmation was taken as reference method. The sensitivity of both tests was 100%, and the specificity of the rapid and standard tests was 96.8% and 88.9% respectively. The modified spot CAMP test is a rapid, inexpensive and accurate method for the identification of group B streptococci, and is more specific than the standard CAMP test.
En este estudio se comparó los resultados de una prueba de CAMP por spot modificada en 20 minutos y la prueba de CAMP original descripta por Christie et al usada para la identificación de Streptococcus agalactiae. Se analizaron 183 aislamientos de estreptococos β hemolíticos, tomando como método de referencia el esquema tradicional de identificación bioquímica y confirmación serológica. La sensibilidad de ambas pruebas fue del 100% y la especificidad de la prueba rápida y la estándar fue de 96,8% y 88,9% respectivamente. La prueba de CAMP por spot modificada es un método rápido, económico y seguro para la identificación de estreptococos del grupo B y posee mayor especificidad que la prueba original.
Subject(s)
Animals , Bacterial Proteins/analysis , Bacterial Typing Techniques/methods , Streptococcus agalactiae/isolation & purification , Agar , Hemolysin Proteins , Hemolysis , Reproducibility of Results , Sensitivity and Specificity , Sheep/bloodABSTRACT
Microbiologic study of bacteremia and fungemia in chronic hemodialysis patients. Bloodstream infections are the second cause of death in patients in chronic hemodialysis (CHD), and the knowledge of the epidemiology is useful to establish proper empiric therapies. The aim of this study was to evaluate the frequency and distribution of microorganisms, in bacteremia and fungemia in 530 patients in CHD. Two hundred and forty eight blood culture series from 114 patients with suspected bacteremia were processed; 44
(n=78) were clinically significative and belonged to 58 patients. Sixty eight percent of these isolates were gram-positive cocci (n:53), and 22
gram-negative rods (n:17). Staphylococcus aureus was the most prevalent pathogen showing 23
of methicillin-resistance. Candida spp. was the fourth pathogen most common in frequency.
ABSTRACT
A rapid modified spot CAMP test using 183 clinical isolates of beta haemolytic streptococci was compared with the standard CAMP test described by Christie et al. The scheme of biochemical identification and serological confirmation was taken as reference method. The sensitivity of both tests was 100
, and the specificity of the rapid and standard tests was 96.8
and 88.9
respectively. The modified spot CAMP test is a rapid, inexpensive and accurate method for the identification of group B streptococci, and is more specific than the standard CAMP test.
ABSTRACT
Microbiologic study of bacteremia and fungemia in chronic hemodialysis patients. Bloodstream infections are the second cause of death in patients in chronic hemodialysis (CHD), and the knowledge of the epidemiology is useful to establish proper empiric therapies. The aim of this study was to evaluate the frequency and distribution of microorganisms, in bacteremia and fungemia in 530 patients in CHD. Two hundred and forty eight blood culture series from 114 patients with suspected bacteremia were processed; 44
of them were positive from which 71
(n=78) were clinically significative and belonged to 58 patients. Sixty eight percent of these isolates were gram-positive cocci (n:53), and 22
gram-negative rods (n:17). Staphylococcus aureus was the most prevalent pathogen showing 23
of methicillin-resistance. Candida spp. was the fourth pathogen most common in frequency.
