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1.
Membranes (Basel) ; 12(11)2022 Nov 02.
Article in English | MEDLINE | ID: mdl-36363644

ABSTRACT

Agricultural waste-based cellulose fibers have gained significant interest for a myriad of applications. Grewia optiva (G. optiva), a plant species, has been widely used for feeding animals, and the small branches' bark is used for making rope. Herein, we have extracted cellulose fibers from the bark of G. optiva species via chemical treatments (including an alkaline treatment and bleaching). The gravimetric analysis revealed that the bark of G. Optiva contains cellulose (63.13%), hemicellulose (13.52%), lignin (15.13%), and wax (2.8%). Cellulose microfibre (CMF) has been synthesized from raw fibre via chemical treatment methods. The obtained cellulose fibers were crosslinked and employed as the matrix to encapsulate the bioactive plant extracts derived from the root of Catharanthus roseus (C. roseus). The microscopic images, XRD, FTIR, and antibacterial/antioxidant activity confirmed the encapsulation of natural extracts in the cellulose microfiber. The microscopic images revealed that the encapsulation of the natural extracts slightly increased the fiber's diameter. The XRD pattern showed that the extracted cellulose microfiber had an average crystalline size of 2.53 nm with a crystalline index of 30.4% compared to the crystalline size of 2.49 nm with a crystalline index of 27.99% for the plant extract incorporated membrane. The water uptake efficiency of the synthesized membrane increased up to 250%. The antimicrobial activity of the composite (the CMF-E membrane) was studied via the zone inhibition against gram-positive and gram-negative bacteria, and the result indicated high antibacterial activity. This work highlighted G. optiva-derived cellulose microfiber as an optimum substrate for antimicrobial scaffolds. In addition, this paper first reports the antimicrobial/antioxidant behavior of the composite membrane of the C. roseus extract blended in the G. optiva microfiber. This work revealed the potential applications of CMF-E membranes for wound healing scaffolds.

2.
Carbohydr Polym ; 251: 117023, 2021 Jan 01.
Article in English | MEDLINE | ID: mdl-33142583

ABSTRACT

Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering. In this study, the limitation was addressed by incorporating regenerated cellulose (rCL) nanofibers into chitosan (CS) hydrogel. The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers. As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix. The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS. The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation. The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold. Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.


Subject(s)
Biocompatible Materials , Cellulose/analogs & derivatives , Chitosan/chemistry , Hydrogels , Osteogenesis , Tissue Engineering , Tissue Scaffolds/chemistry , Animals , Biocompatible Materials/chemistry , Bone Regeneration , Cell Differentiation , Cell Line , Cellulose/chemistry , Hydrogels/chemistry , Mechanical Phenomena , Mice , Nanofibers/chemistry , Osteoclasts
3.
Int J Biol Macromol ; 164: 976-985, 2020 Dec 01.
Article in English | MEDLINE | ID: mdl-32710964

ABSTRACT

We present an integrated design and fabrication strategy for the development of hierarchically structured biomechanically and biologically functional tissue scaffold. An integration of ß-TCP incorporated fluffy type nanofibers and biodegradable interpenetrating gelatin-hydrogel networks (IGN) result in biomimetic tissue engineered constructs with fully tunable properties that can match specific tissue requirements. FESEM images showed that nanofibers were efficiently assembled into an orientation of IGN without disturbing its pore architecture. The pore architecture, compressive stiffness and modulus, swelling, and the biological properties of the composite constructs can be tailored by adjusting the composition of nanofiber content with respect to IGN. Experimental results of cell proliferation assay and confocal microscopy imaging showed that the as-fabricated composite constructs exhibit excellent ability for MC3T3-E1 cell proliferation, infiltration and growth. Furthermore, ß-TCP incorporated functionalized nanofiber enhanced the biomimetic mineralization, cell infiltration and cell proliferation. Within two weeks of cell-seeding, the composite construct exhibited enhanced osteogenic performance (Runx2, osterix and ALP gene expression) compared to pristine IGN hydrogel scaffold. Our integrated design and fabrication approach enables the assembly of nanofiber within IGN architecture, laying the foundation for biomimetic scaffold.


Subject(s)
Calcium Phosphates/chemistry , Hydrogels , Nanofibers/chemistry , Polyesters/chemistry , Tissue Engineering/methods , Tissue Scaffolds , 3T3 Cells , Alkaline Phosphatase/chemistry , Animals , Biomechanical Phenomena , Biomimetics , Cell Proliferation , Core Binding Factor Alpha 1 Subunit/chemistry , Gelatin , Mice , Osteoblasts/cytology , Osteogenesis , Sp7 Transcription Factor/chemistry
4.
ACS Appl Mater Interfaces ; 12(16): 18197-18210, 2020 Apr 22.
Article in English | MEDLINE | ID: mdl-32153182

ABSTRACT

A bioinspired three-dimensional (3D) fibrous structure possesses biomimicry, valuable functionality, and performance to scaffolding in tissue engineering. In particular, an electrospun fibrous mesh has been studied as a scaffold material in various tissue regeneration applications. We produced a low-density 3D polycaprolactone/lactic acid (LA) fibrous mesh (3D-PCLS) via the novel lactic-assisted 3D electrospinning technique exploiting the catalytic properties of LA as we reported previously. In the study, we demonstrated a strategy of recycling the LA component to synthesize the osteoinductive biomolecules in situ, calcium lactate (CaL), thereby forming a 3D bioactive PCL/CaL fibrous scaffold (3D-SCaL) for bone tissue engineering. The fiber morphology of 3D-PCLS and its packing degree could have been tailored by modifying the spinning solution and the collector design. 3D-SCaL demonstrated successful conversion of CaL from LA and exhibited the significantly enhanced biomineralization capacity, cell infiltration and proliferation rate, and osteoblastic differentiation in vitro with two different cell lines, MC3T3-e1 and bone marrow stem cells. In conclusion, 3D-SCaL proves to be a highly practical and accessible strategy using a variety of polymers to produce 3D fibers as a potential candidate for future regenerative medicine and tissue engineering applications.


Subject(s)
Bone and Bones , Calcium Compounds , Lactates , Lactic Acid , Tissue Engineering/instrumentation , Tissue Scaffolds/chemistry , Animals , Bone and Bones/cytology , Bone and Bones/metabolism , Calcium Compounds/chemistry , Calcium Compounds/metabolism , Calcium Compounds/pharmacology , Cell Differentiation/drug effects , Cell Line , Cell Proliferation/drug effects , Cells, Cultured , Electrochemical Techniques , Lactates/chemistry , Lactates/metabolism , Lactates/pharmacology , Lactic Acid/chemistry , Lactic Acid/metabolism , Mice , Osteoblasts/cytology , Osteoblasts/drug effects , Polyesters/chemistry , Rabbits
5.
J Phys Chem Lett ; 10(10): 2400-2407, 2019 May 16.
Article in English | MEDLINE | ID: mdl-31017440

ABSTRACT

Millimeter-sized CsPbBr3 single crystals were prepared via a facile solvent-evaporation method in ambient environment. The heterojunction between p-type CuI and n-type CsPbBr3 was formed by a simple immersion process. The as-integrated CsPbBr3/CuI device exhibits a good rectifying behavior (ratio of 250 at ±2 V). In particular, the photodetector shows excellent self-powered characteristics under 540 nm light illumination, including high photocurrent (near 100 nA); high photosensitivity (on/off ratio of 1.5 × 103); fast response speed (0.04/2.96 ms); and good wavelength selectivity (565-525 nm), responsivity (1.4 mA W-1), and detectivity (6.2 × 1010 Jones). This work provides a simple, low-cost, and effective method for preparing millimeter-level CsPbBr3 single crystals. The simple device architecture further provides a promising approach for fabricating high-performance self-powered photodetectors.

6.
Carbohydr Polym ; 212: 21-29, 2019 May 15.
Article in English | MEDLINE | ID: mdl-30832849

ABSTRACT

Synthetic polymers are easy to process and have excellent mechanical properties but low wettability and poor cell compatibility limit their applications in tissue scaffolding. In this study, a facile procedure was established to regenerate cellulose and calcium lactate (CaL) into a polycaprolactone (PCL) nanofibrous scaffold for tissue engineering applications. Briefly, varying amounts of lactic acid (LA) was mixed with the blend of PCL and cellulose acetate (CA) solutions and electrospun to fabricate an optimal composite PCL/CA/LA fibrous membrane. Later on, as-prepared membranes were treated with calcium hydroxide solution. This process simultaneously converted CA and LA contents into Cellulose and CaL, respectively. In situ regeneration of Cellulose and CaL into the composite fiber remarkably enhanced the biological and physicochemical properties of the composite fiber. This work provides a novel dual-channel strategy for simultaneous regeneration of biopolymer and bioactive molecule into the PCL nanofiber for regenerative medicine and tissue engineering applications.


Subject(s)
Biocompatible Materials/chemical synthesis , Calcium Compounds/chemical synthesis , Cellulose/chemical synthesis , Chemistry, Pharmaceutical/methods , Lactates/chemical synthesis , Nanofibers/chemistry , Polyesters/chemical synthesis , Biocompatible Materials/pharmacology , Calcium Compounds/pharmacology , Cell Proliferation/drug effects , Cell Proliferation/physiology , Cellulose/pharmacology , Humans , Lactates/pharmacology , Nanofibers/administration & dosage , Polyesters/pharmacology
7.
ACS Appl Mater Interfaces ; 10(24): 20256-20270, 2018 Jun 20.
Article in English | MEDLINE | ID: mdl-29808986

ABSTRACT

Localized drug-delivery systems (LDDSs) are a promising approach for cancer treatment because they decrease systematic toxicity and enhance the therapeutic effect of the drugs via site-specific delivery of active compounds and possible gradual release. However, the development of LDDS with rationally controlled drug release and intelligent functionality holds great challenge. To this end, we have developed a tailorable fibrous site-specific drug-delivery platform functionalized with pH- and near-infrared (NIR)-responsive polypyrrole (PPy), with the aim of cancer treatment via a combination of photothermal ablation and chemotherapy. First, a paclitaxel (PTX)-loaded polycaprolactone (PCL) (PCL-PTX) mat was prepared by electrospinning and subsequently in situ membrane surface-functionalized with different concentrations of PPy. The obtained PPy-functionalized mats exhibited excellent photostability and heating property in response to NIR exposure. PPy-coated mats exhibited enhanced PTX release in a pH 5.5 environment compared to pH 7.4. Release was further accelerated in response to NIR under both conditions; however, superior release was observed at pH 5.5 compared to pH 7.4, indicating a dual stimuli-responsive (pH and NIR) drug-delivery platform. More importantly, the 808 nm NIR irradiation enabled markedly accelerated PTX release from PPy-coated PCL-PTX mats and slowed and sustained release following termination of laser irradiation, confirming representative stepwise drug-release properties. PPy-coated PCL-PTX mats presented significantly enhanced in vitro and in vivo anticancer efficacy under NIR irradiation compared to PPy-coated PCL-PTX mats not exposed to NIR or uncoated mats (PCL-PTX). This study has thus developed a promising fibrous site-specific drug-delivery platform with NIR- and pH-triggering that notably utilizes PPy as a dopant for synergistic photothermal chemotherapy.


Subject(s)
Polymers/chemistry , Pyrroles/chemistry , Drug Delivery Systems , Humans , Hydrogen-Ion Concentration , Infrared Rays , Neoplasms
8.
J Nanosci Nanotechnol ; 18(1): 529-537, 2018 01 01.
Article in English | MEDLINE | ID: mdl-29768878

ABSTRACT

Enhancing the biocompatibility profiles including cell attachment, growth, and viability and mechanical properties of designed synthetic scaffolds have an essential role in tissue engineering applications. Polymer blending is one of the most effective methods for providing new anticipated biomaterials for tissue scaffolds. Here, the blend solution of the different mass weight ratio of polycaprolactone (PCL) to human serum albumin (HSA) was subjected to fabricate nanocomposite spider-web-like membranes using electrospinning process. The physicochemical aspects of fabricated membranes had been characterized by a different state of techniques like that of scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), contact angle meter and universal testing machine. FE-SEM images revealed that all PCL/HSA mats were composed of interlinked nano-nets along with conventional electrospun fibers while nano-nets were not found for pristine PCL mat. Moreover, composite membranes exhibited improved water absorbability, enhanced biodegradation compared to pristine PCL membrane and had much better mechanical properties (tensile strength increased by up to 3-fold, Young's modulus by 2-fold). The cell attachment and proliferation tests were carried by culturing Mc3T3-E1 (pre-osteoblasts) with the designated nanofibrous membranes. The hybrid nanofibers exhibited extraordinary support for the adhesion and proliferation of cells when compared to the pristine PCL membrane. These results indicate that the nano-nets supported PCL/HSA scaffolds can be promising for tissue engineering applications.


Subject(s)
Biocompatible Materials , Nanofibers , Tissue Engineering , Cell Proliferation , Humans , Polyesters , Spectroscopy, Fourier Transform Infrared , Tissue Scaffolds
9.
J Colloid Interface Sci ; 513: 566-574, 2018 Mar 01.
Article in English | MEDLINE | ID: mdl-29190568

ABSTRACT

Depositing of hierarchical ZnO nanostructures on electrospun nanofibers and their proper attachment has gained significant interest for myriad applications. However, the weak attachment of such nanostructures to the nanofiber surface limits their practical applications. In this study, a simple and efficient method has been developed for preparing hierarchical ZnO nanorod deposited polyurethane (PU) nanofiber by combining electrospinning, surface functionalization and hydrothermal treatment. Electrospun PU nanofibers were coated with polydopamine (Pdopa) via dip coating method. The resulting Pdopa coated PU nanofibrous mat was soaked in aqueous ZnO nanoparticles (ZnONPs) solution in order to seed the metal-oxide particles on its surface. Later, ZnO nanorods (ZNRs) were grown on the ZnO-seeded electrospun PU nanofiber via a hydrothermal process. X-ray photoelectron spectroscopy (XPS), Field-Emission Scanning electron microscopy (FE-SEM), X-ray diffraction pattern (XRD) and infra-red (IR) spectra indicated that ZnO nanorods firmly adhered to the functionalized PU nanofiber surface and had high photocatalytic/antimicrobial activity at the low-intensity UV-LED device with good reusability. The catechol group of Pdopa not only causes adhesion of ZnO nanostructures, but also act as an electron trap, preventing the recombination of e-h pairs and thereby improving the photocatalytic efficiency. We believe that the fabricated composite membrane with antifouling effect and photocatalytic activity is a potential candidate for organic pollutant degdration and wastewater purification.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Indoles/chemistry , Nanofibers/administration & dosage , Nanostructures/chemistry , Photochemistry , Polymers/chemistry , Zinc Oxide/chemistry , Anti-Bacterial Agents/chemistry , Catalysis , Electroplating , Materials Testing , Nanofibers/chemistry , Nanofibers/radiation effects , Nanostructures/radiation effects , Zinc Oxide/radiation effects
10.
J Mech Behav Biomed Mater ; 65: 66-76, 2017 01.
Article in English | MEDLINE | ID: mdl-27552600

ABSTRACT

Silver nanoparticles embedded within a nanofibrous polymer matrix have significant attention in recent years as an antimicrobial wound dressing materials. Herein, we have fabricated a novel Ag-polyurethane-zein hybrid nanofibrous scaffold for wound dressing applications. AgNPs were synthesized in-situ via reduction of silver nitrate in electrospinning solution. Varying mass composition of the components showed the pronounced effect on the morphology and physicochemical properties of the composite fibers. Field-Emission Scanning Electron Microscopy (FESEM) images revealed that PU and zein with mass ratio 2:1 produced the bead-free continuous and uniformly distributed nanofibers. Fourier-transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric Analysis (TGA) confirmed the well interaction between component polymers. Compared to the pristine PU nanofibers, composite fibers showed enhanced tensile strength, young׳s modulus and surface wettability. The antibacterial capacity of the nanofibrous membrane was evaluated against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacterial strains via a zone of inhibition test, and the results showed high antibacterial performance for Ag incorporated composite mat. Experimental results of cell viability assay and microscopic imaging revealed that as-fabricated scaffolds have an excellent ability for fibroblast cell adhesion, proliferation and growth. Overall, as-fabricated antibacterial natural/synthetic composite scaffold can be a promising substrate for repairing skin defects.


Subject(s)
Anti-Infective Agents/pharmacology , Bandages , Metal Nanoparticles/analysis , Nanofibers/analysis , Silver/pharmacology , Escherichia coli/drug effects , Staphylococcus aureus/drug effects , Tissue Scaffolds , X-Ray Diffraction
11.
J Colloid Interface Sci ; 476: 29-34, 2016 Aug 15.
Article in English | MEDLINE | ID: mdl-27179176

ABSTRACT

We report for the first time a polycaprolactone-human serum albumin (PCL-HSA) membrane with bimodal structures comprised of spider-web-like nano-nets and conventional fibers via facile electro-spinning/netting (ESN) technique. Such unique controllable morphology was developed by electrospinning the blend solution of PCL (8wt% in HFIP 1,1,1,3,3,3,-Hexafluoro-2-propanol) and HSA (10wt% deionized water). The phase separation during electrospinning caused the formation of bimodal structure. Various processing factors such as applied voltage, feeding rate, and distance between nozzle tip and collector were found responsible for the formation and distribution of the nano-nets throughout the nanofibrous mesh. Field emission electron microscopy (FE-SEM) confirmed that the nano-nets were composed of interlinked nanowires with an ultrathin diameter (10-30nm). When compared with a pure PCL membrane, the membrane containing nano-nets was shown to have better support for cellular activities as determined by cell viability and attachment assays. These results revealed that the blending of albumin, a hydrophilic biomolecule, with PCL, a hydrophobic polymer, proves to be an outstanding approach to developing membranes with controlled spider-web-like nano-nets for tissue engineering.


Subject(s)
Biocompatible Materials/chemistry , Polyesters/chemistry , Serum Albumin/chemistry , Tissue Engineering , Animals , Cell Line , Cell Survival , Humans , Mice , Particle Size , Surface Properties
12.
Carbohydr Polym ; 147: 104-113, 2016 08 20.
Article in English | MEDLINE | ID: mdl-27178914

ABSTRACT

The aim of the present study is to develop a facile, efficient approach to reinforce nylon 6 (N6) nanofibers with cellulose chains as well as to study the effect that cellulose regeneration has on the physicochemical properties of the composite fibers. Here, a cellulose acetate (CA) solution (17wt%) was prepared in formic acid and was blended with N6 solution (20%, prepared in formic acid and acetic acid) in various proportions, and the blended solutions were then electrospun to produce hybrid N6/CA nanofibers. Cellulose was regenerated in-situ in the fiber via alkaline saponification of the CA content of the hybrid fiber, leading to cellulose-reinforced N6 (N6/CL) nanofibers. Electron microscopy studies suggest that the fiber diameter and hence pore size gradually decreases as the mass composition of CA increases in the electrospinning solution. Cellulose regeneration showed noticeable change in the polymorphic behavior of N6, as observed in the XRD and IR spectra. The strong interaction of the hydroxyl group of cellulose with amide group of N6, mainly via hydrogen bonding, has a pronounced effect on the polymorphic behavior of N6. The γ-phase was dominant in pristine N6 and N6/CA fibers while α- phase was dominant in the N6/CL fibers. The surface wettability, wicking properties, and the tensile stress were greatly improved for N6/CL fibers compared to the corresponding N6/CA hybrid fibers. Results of DSC/TGA revealed that N6/CL fibers were more thermally stable than pristine N6 and N6/CA nanofibers. Furthermore, regeneration of cellulose chain improved the ability to nucleate bioactive calcium phosphate crystals in a simulated body fluid solution.


Subject(s)
Biomimetics , Caprolactam/analogs & derivatives , Nanofibers/chemistry , Polymers/chemistry , Capillary Action , Caprolactam/chemistry , Cellulose/chemistry , Temperature
13.
J Mech Behav Biomed Mater ; 60: 312-323, 2016 07.
Article in English | MEDLINE | ID: mdl-26919567

ABSTRACT

The objective of the current work is to incorporate calcium lactate (CL) into polycaprolactone (PCL)/zein composite micro/nanofibrous scaffolds via electrospinning to engineer bone tissue. In this study, a composite micro/nano fibrous scaffold was fabricated using a single two-nozzle electrospinning system to combine indicative nanofibers from a blended solution of zein-CL and micro-sized fibers from a PCL solution. Incorporation of the CL into the PCL/zein fibers were shown to improve the wettability, tensile strength and biological activity of the composite mats. Moreover, the composite mats have a high efficiency to nucleate calcium phosphate from simulated body fluid (SBF) solution. An in vitro cell culture with osteoblast cells demonstrated that the electrospun composite mats possessed improved biological properties, including a better cell adhesion, spread and proliferation. This study has demonstrated that the PCL/zein-CL composite provides a simple platform to fabricate a new biomimetic scaffold for bone tissue engineering, which can recapitulate both the morphology of extracellular matrix and composition of the bone.


Subject(s)
Calcium Compounds/chemistry , Lactates/chemistry , Polyesters/chemistry , Tissue Engineering , Tissue Scaffolds , Zein/chemistry , Cells, Cultured , Humans , Osteoblasts/cytology
14.
Carbohydr Polym ; 136: 154-62, 2016 Jan 20.
Article in English | MEDLINE | ID: mdl-26572341

ABSTRACT

In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h. The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution. In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge. SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane. 3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution. SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone. 3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat. Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.


Subject(s)
Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Biomimetics , Calcification, Physiologic/drug effects , Cellulose/analogs & derivatives , Nanofibers/chemistry , Tissue Scaffolds/chemistry , Borohydrides/chemistry , Cell Proliferation/drug effects , Cellulose/chemistry , Electricity , Ethanol/chemistry , Membranes, Artificial , Sodium Hydroxide/chemistry , Tissue Engineering
15.
ACS Appl Mater Interfaces ; 7(35): 19672-83, 2015 Sep 09.
Article in English | MEDLINE | ID: mdl-26295953

ABSTRACT

Post-electrospinning treatment is a facile process to improve the properties of electrospun nanofibers for various applications. This technique is commonly used when direct electrospinning is not a suitable option to fabricate a nonwoven membrane of the desired polymer in a preferred morphology. In this study, a representative natural-synthetic hybrid of cellulose acetate (CA) and polycaprolactone (PCL) in different ratios was fabricated using an electrospinning process, and CA in the hybrid fiber was transformed into cellulose (CL) by post-electrospinning treatment via alkaline saponification. Scanning electron microscopy was employed to study the effects of polymer composition and subsequent saponification on the morphology of the nanofibers. Increasing the PCL content in the PCL/CA blend solution caused a gradual decrease in viscosity, resulting in smoother and more uniform fibers. The saponification of fibers lead to pronounced changes in the physicochemical properties. The crystallinity of the PCL in the composite fiber was varied according to the composition of the component polymers. The water contact angle was considerably decreased (from 124° to less than 20°), and the mechanical properties were greatly enhanced (Young's Modulus was improved by ≈20-30 fold, tensile strength by 3-4 fold, and tensile stress by ≈2-4 fold) compared to those of PCL and PCL/CA membranes. Regeneration of cellulose chains in the nanofibers increased the number of hydroxyl groups, which increased the hydrogen bonding, thereby improving the mechanical properties and wettability of the composite nanofibers. The improved wettability and presence of surface functional groups enhanced the ability to nucleate bioactive calcium phosphate crystals throughout the matrix when exposed to a simulated body fluid solution. Experimental results of cell viability assay, confocal microscopy, and scanning electron microscopy imaging showed that the fabricated nanofibrous membranes have excellent ability for MC3T3-E1 cell proliferation and growth. Given the versatility and widespread use of cellulose-synthetic hybrid systems in the construction of tissue-engineered scaffolds, this work provides a novel strategy to fabricate the biopolymer-based materials for applications in tissue engineering and regenerative medicine.


Subject(s)
Cellulose/analogs & derivatives , Nanofibers/chemistry , Nanoparticles/chemistry , Polyesters/chemistry , Animals , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Cell Line , Cell Survival/drug effects , Cellulose/chemistry , Durapatite/chemistry , Elastic Modulus , Mice , Nanofibers/toxicity , Nanofibers/ultrastructure , Tissue Engineering , Tissue Scaffolds
16.
J Colloid Interface Sci ; 453: 159-168, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-25985419

ABSTRACT

In this study, a fly ash based composite, Ag-iron oxide/fly ash, was synthesized via a facile one-pot hydrothermal process using fly ash, ferrous chloride, and silver nitrate as precursors. Field emission scanning electron microscopy (FE-SEM), EDX, transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FTIR), Photoluminescence (PL) and Brunauer-Emmett-Teller (BET) surface area measurement confirmed the formation of composite particle. FA provided a suitable surface for the in-situ deposition of Fe3O4 and Ag NPs during hydrothermal treatment. As a result, the particle size of Fe3O4 and Ag NPs was sufficiently decreased, and the surface area of the NPs as well as, a whole matrix was increased. The antimicrobial activity of the composite was accessed by Escherichia coli inhibition assay. Lead(II) ion adsorption efficiency of the composite was analyzed from a series of batch adsorption experiments (the effects of concentration, contact time, pH and adsorbent dose on the adsorption of Pb(II) ion from aqueous solution). Results indicated that as-synthesized composite has high antibacterial capacity, and the metal ions uptake efficiency compared to fly ash particle. Furthermore, incorporation Fe3O4 NPs onto the fly ash make it easily separable from a reaction system using an external magnet. The composite synthesis protocol is a simple method that utilizes a readily available industrial byproduct to produce a unique composite for environmental remediation.


Subject(s)
Anti-Infective Agents/chemistry , Coal Ash/chemistry , Ferric Compounds/chemistry , Lead/isolation & purification , Silver/chemistry , Water Pollutants, Chemical/isolation & purification , Water Purification/methods , Adsorption , Anti-Infective Agents/pharmacology , Coal Ash/pharmacology , Coloring Agents , Escherichia coli/drug effects , Escherichia coli/growth & development , Ferric Compounds/pharmacology , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Nanoparticles/chemistry , Nanoparticles/ultrastructure , Silver/pharmacology , Silver Nitrate/chemistry , Water Microbiology , X-Ray Diffraction
17.
J Hazard Mater ; 264: 25-33, 2014 Jan 15.
Article in English | MEDLINE | ID: mdl-24269971

ABSTRACT

A stable silver-doped fly ash/polyurathene (Ag-FA/PU) nanocomposite multifunctional membrane is prepared by a facile one-step electrospinning process using fly ash particles (FAPs). Colloidal solution of PU with FAPs and Ag metal precursor was subjected to fabricate nanocomposite spider-web-like membrane using electrospinning process. Presence of N,N-dimethylformamide (solvent of PU) led to reduce silver nitrate into Ag NPs. Incorporation of Ag NPs and FAPs through electrospun PU fibers is proven through electron microscopy and spectroscopic techniques. Presence of these NPs on PU nanofibers introduces several potential physicochemical properties such as spider-web-like nano-neeting for NPs separation, enhanced absorption capacity to remove carcinogenic arsenic (As) and toxic organic dyes, and antibacterial properties with reduce bio-fouling for membrane filter application. Preliminary observations used for above-mentioned applications for water treatment showed that it will be an economically and environmentally friendly nonwoven matrix for water purification. This simple approach highlights new avenues about the utilization of one pollutant material to control other pollutants in scalable and inexpensive ways.


Subject(s)
Membranes, Artificial , Nanostructures/chemistry , Polyurethanes/chemistry , Water Purification/instrumentation , Adsorption
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