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2.
J Dairy Sci ; 100(8): 6090-6099, 2017 Aug.
Article in English | MEDLINE | ID: mdl-28571988

ABSTRACT

Spore-forming bacteria, principally Bacillus species, are important contaminants of milk. Because of their high heat resistance, Bacillus species spores are capable of surviving the heat treatment process of milk and lead to spoilage of the final product. To determine the factors influencing the contamination of milk, spore-forming bacteria occurrence throughout the UHT milk production line during winter, spring, and summer was studied. The obtained results confirm that the total viable rate decreases rapidly throughout the production line of UHT milk showing the efficiency of thermal treatments used. However, the persistent high rate of spore-forming bacteria indicates their high heat resistance, especially in spring and summer. In addition, a significant variation of the quality of raw milk according to the location of the collecting centers was revealed. The molecular identification showed a high degree of diversity of heat-resistant Bacillus species, which are isolated from different milk samples. The distribution of Bacillus species in raw milk, stored milk, bactofuged milk, pasteurized milk, and UHT milk were 28, 10, 16, 13, and 33%, respectively. Six Bacillus spp. including Bacillus licheniformis (52.38%), Bacillus pumilus (9.52%), Bacillus sp. (4.76%), Bacillus sporothermodurans (4.76%), Terribacillus aidingensis (4.76%), and Paenibacillus sp. (4.76%) were identified in different milk samples.


Subject(s)
Bacillus/physiology , Food Microbiology , Hot Temperature , Milk/microbiology , Spores, Bacterial/growth & development , Animals , Seasons , Tunisia
3.
Lett Appl Microbiol ; 64(5): 318-334, 2017 May.
Article in English | MEDLINE | ID: mdl-28208218

ABSTRACT

In the last few years, different surveillances have been published in Africa, especially in northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data show a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmid-mediated quinolone resistance genes and extended spectrum ß-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys. Within this group, CTX-M-15 was the main variant both in animals and humans, except in Tunisia where CTX-M-1 was more frequently detected among Escherichia coli from poultry. Certain blaCTX-M-15 -harbouring clones (ST131/B2 or ST405/D) are mainly identified in humans, but they have also been reported in livestock species from Tanzania, Nigeria or Tunisia. Moreover, several reports suggest an inter-host circulation of specific plasmids (e.g. blaCTX-M-1 -carrying IncI1/ST3 in Tunisia, IncY- and Inc-untypeable replicons co-harbouring qnrS1 and blaCTX-M-15 in Tanzania and the worldwide distributed blaCTX-M-15 -carrying IncF-type plasmids). International trade of poultry meat seems to have contributed to the spread of other ESBL variants, such as CTX-M-14, and clones. Furthermore, first descriptions of OXA-48- and OXA-181-producing E. coli have been recently documented in cattle from Egypt, and the emergent plasmid-mediated colistin resistance mcr-1 gene has been also identified in chickens from Algeria, Tunisia and South Africa. These data reflect the urgent need of a larger regulation in the use of veterinary drugs and the implementation of surveillance programmes in order to decelerate the advance of antimicrobial resistance in this continent.


Subject(s)
Cattle Diseases/microbiology , Drug Resistance, Microbial/genetics , Escherichia coli Infections/veterinary , Escherichia coli/drug effects , Escherichia coli/genetics , Poultry Diseases/microbiology , Algeria , Animals , Anti-Bacterial Agents/pharmacology , Cattle , Chickens/microbiology , Egypt , Escherichia coli Proteins/genetics , Humans , Nigeria , Poultry/microbiology , South Africa , Tunisia , beta-Lactamases/genetics
4.
Eur J Clin Microbiol Infect Dis ; 31(7): 1511-6, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22065280

ABSTRACT

The objective of this investigation was to analyse the carriage rate of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in faecal samples of healthy humans in Tunisia and to characterise the recovered isolates. One hundred and fifty samples were inoculated on MacConkey agar plates supplemented with cefotaxime (2 µg/ml) for ESBL-positive E. coli recovery. The characterisation of ESBL genes and their genetic environments, detection of associated resistance genes, multilocus sequence typing (MLST) and phylogroup typing were performed by polymerase chain reaction (PCR) and sequencing. The presence and characterisation of integrons and virulence factors were studied by PCR and sequencing. ESBL-positive E. coli isolates were detected in 11 of 150 faecal samples (7.3%) and one isolate/sample was further characterised. These isolates contained the blaCTX-M-1 (ten isolates) and blaTEM-52c genes (one isolate). The ISEcp1 (truncated by IS10 in four strains) and orf477 sequences were found upstream and downstream, respectively, of all bla (CTX-M-1) genes. Seven different sequence types (STs) and three phylogroups were identified among CTX-M-1-producing isolates [ST/phylogroup (number of isolates)]: ST58/B1 (3), ST57/D (2), ST165/A (1), ST155/B1 (1), ST10/A (1), ST398/A (1) and ST48/B1 (1). The TEM-52-producing isolate was typed as ST219 and phylogroup B2. Six ESBL isolates contained class 1 integrons with the gene cassettes dfrA17-aadA5 (five isolates) and dfrA1-aadA1 (one). Healthy humans in the studied country could be a reservoir of CTX-M-1-producing E. coli.


Subject(s)
Carrier State/epidemiology , Carrier State/microbiology , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Escherichia coli/enzymology , Escherichia coli/isolation & purification , beta-Lactamases/genetics , Adolescent , Adult , Aged , Animals , Child , Child, Preschool , Escherichia coli/genetics , Female , Healthy Volunteers , Humans , Infant , Male , Middle Aged , Polymerase Chain Reaction , Prevalence , Sequence Analysis, DNA , Tunisia/epidemiology , Young Adult
5.
Eur J Clin Microbiol Infect Dis ; 30(4): 499-508, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21076928

ABSTRACT

Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3')-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3')-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals.


Subject(s)
Carrier State/epidemiology , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Nasal Mucosa/microbiology , Staphylococcal Infections/epidemiology , Staphylococcus aureus/isolation & purification , Virulence Factors/genetics , Animals , Bacterial Proteins/genetics , Bacterial Typing Techniques , Carrier State/microbiology , Drug Resistance, Multiple, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Humans , Methicillin/pharmacology , Methicillin Resistance/genetics , Methicillin-Resistant Staphylococcus aureus/drug effects , Methicillin-Resistant Staphylococcus aureus/genetics , Methicillin-Resistant Staphylococcus aureus/pathogenicity , Microbial Sensitivity Tests , Risk Factors , Staphylococcal Infections/microbiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/genetics , Staphylococcus aureus/pathogenicity , Tunisia/epidemiology , Virulence
6.
J Chemother ; 22(5): 318-23, 2010 Oct.
Article in English | MEDLINE | ID: mdl-21123154

ABSTRACT

Beta-lactamase characterization was carried out in a collection of 18 extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates from blood (n=8) and urine (n=10) obtained in 2007 in a tunisian Hospital. All isolates were clonally unrelated according to PFGE analysis. Seventeen strains presented the bla(CTX-M-)15 gene associated with bla (OXA-)1 and four of these strains with the (TEM-)1(b) gene. The remaining ESBL-positive strain contained the bla (CTX-M-)9 gene associated with the bla (OXA-)1 and bla (TEM-)1(b) genes. The orf477 sequence was identified downstream of the bla(CTX-M-)15 gene in all 17 bla(CTX-M-)15-positive strains, and ISEcp1 upstream in 15 of them (in eight cases truncated by IS26). The presence of a class 1 integron was demonstrated in 4 of the 18 ESBL-positive strains (22.2%), with dfrA17 + aadA5 (3 strains) and dfrA12 + orfF + aadA2 (1 strain) being the gene cassettes identified. The variant aac(6´)-Ib-cr was found in 15 bla(CTX-M-)15-containing strains. All 18 ESBL-positive strains were typed as phylogroup B2 and contained at least three of the eight tested virulence genes (fimA, papGIII, hlyA, cnf1, papC, aer, eae and bfp). Six bla(CTX-M-)15-positive strains were included in the serotype O25b and one of them was typed as ST131. Another bla(CTX-M-)15-positive strain serotype-O25 was typed as ST638. The bla(CTX-M-)15, aac(6')- Ib-cr, and aac(3)-II genes were co-transferred by conjugation from 7 donor strains to E. coli CSH26 recipient strain. The bla(CTXM-)15 gene is prevalent among ESBL-positive E. coli strains in the studied hospital, that is frequently found together with aac(6')- Ib-cr, and aac(3)-II genes. The detection of the clone O25b-St131 in a bla(CTX-M-)15 strain corroborates its worldwide dissemination.


Subject(s)
Escherichia coli Infections/microbiology , Escherichia coli/enzymology , Escherichia coli/genetics , Integrons , Virulence Factors/genetics , beta-Lactamases/genetics , beta-Lactamases/metabolism , Blood/microbiology , Conjugation, Genetic , Electrophoresis, Gel, Pulsed-Field , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Humans , Microbial Sensitivity Tests , Tunisia , Urine/microbiology , beta-Lactam Resistance/genetics , beta-Lactamases/biosynthesis
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