Evaluation of blood lead levels in opium addicts and healthy control group using novel deep eutectic solvent based dispersive liquid-liquid microextraction followed by GFAAS.

Today, drug dealers and sellers add lead compounds to these substances to get more profit. As a result, drug users are heavily exposed to lead, and lead poisoning is clearly seen in most of them. Therefore, it is especially important to check the blood lead levels in these people. In this research, an efficient and eco-friendly pretreatment method was established by deep eutectic solvent for dispersive liquid-liquid microextraction (DES - DLLME) followed by graphite furnace atomic absorption spectrometry (GFAAS) analysis. The selected hydrophilic deep eutectic solvent consists of l-menthol and (1S)-( +)-camphor-10-sulfonic acid (CSA) at a 5:1 molar ratio as a green solvent instead of traditional toxic organic solvents. Under the optimal extraction conditions, the introduced method exhibited good linearity with coefficient of determination (r2) 0.9975 and an acceptable linear range of 0.3-80 µg L-1. Accordingly, the detection limit was 0.1 µg L-1 (S/N = 3) for lead ions, and the high enrichment factor (240) was obtained. The proposed method was successfully applied to analysis lead ions in real blood samples, which is a promising technique for biological samples. The case samples were classified and analyzed based on age, duration of consumption, and type of substance. The results showed that there was no significant difference between blood lead levels in different age groups and different duration of use, while blood lead levels were higher in opium residue (shireh) users than in opium users.

Trace determination of triazine herbicides in fruit and vegetables using novel hydrophobic deep eutectic solvent-based dispersive liquid-liquid microextraction followed by high-performance liquid chromatography-ultraviolet.

In the present research, a novel hydrophobic deep eutectic solvent-based dispersive liquid-liquid microextraction technique was established and combined with high-performance liquid chromatography-ultraviolet for the determination of triazine herbicides in fruit and vegetable samples. A deep eutectic solvent was synthesized using l-menthol as a hydrogen bond acceptor and ethylene glycol as a hydrogen bond donor and used as a green extractant. The characterization of deep eutectic solvent was investigated by Fourier-transform infrared, nuclear magnetic resonance, and thermogravimetric analysis. Under the optimum conditions, relative standard deviation values for intra-day and inter-day of the method based on seven replicate measurements of 50.0 µg/kg of triazines were in the range of 2.8%-5.5% and 3.7%-7.2%, respectively. The calibration graphs were linear in the range of 3.0-500 µg/kg and the limits of detection were in the range of 1.0-2.0 µg/kg. The relative recoveries of different fruit and vegetable samples that have been spiked with two levels of target compounds were 91.5%-109.8%. The method has good linearity, sensitivity, accuracy, and precision. It is also environmentally friendly and was successfully used to determine the concentrations of triazines in fruit and vegetable samples.