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1.
J Proteome Res ; 7(3): 1067-77, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18271526

ABSTRACT

To identify phosphotyrosine (pY) sites in the epidermal growth factor receptor (EGFR) network, a tandem immunoprecipitation-mass spectrometry method (TIPY-MS) was applied wherein protease-digested EGFR immune complexes were extracted with anti-pY after Rush et al. ( Nat. Biotech. 2005, 23, 94 ) and analyzed by LC-MS/MS. New pY sites in the pathway were found, including SOS1 Y1065, SOS2 Y1275, CBL-B Y889, and in the EGFR regulatory protein Mig-6 Y458. The novel human C19orf19 gene product was found EGFR-associated and phosphorylated at 5 tyrosines in response to EGFR activation and, therefore, represents a new component of the EGFR signaling network.


Subject(s)
ErbB Receptors/metabolism , Mass Spectrometry/methods , Phosphotyrosine/chemistry , Amino Acid Sequence , Cell Line , Humans , Immunoprecipitation , Molecular Sequence Data , Open Reading Frames , Phosphorylation
2.
J Cell Sci ; 120(Pt 11): 1888-97, 2007 Jun 01.
Article in English | MEDLINE | ID: mdl-17504806

ABSTRACT

During animal cell chemotaxis, signalling at the plasma membrane induces actin polymerisation to drive forward cell movement. Since the cellular pool of actin is limited, efficient protrusion formation also requires the coordinated disassembly of pre-existing actin filaments. To search for proteins that can monitor filamentous and globular actin levels to maintain the balance of polymerisation and disassembly, we followed changes in the proteome induced by RNA interference (RNAi)-mediated alterations in actin signalling. This unbiased approach revealed an increase in the levels of an inactive, phosphorylated form of the actin-severing protein cofilin in cells unable to generate actin-based lamellipodia. Conversely, an increase in F-actin levels induced the dephosphorylation and activation of cofilin via activation of the Ssh phosphatase. Similarly, in the context of acute phosphoinositide 3-kinase (PI3K) signalling, dynamic changes in cofilin phosphorylation were found to depend on the Ssh phosphatase and on changes in lamellipodial F-actin. These results indicate that changes in the extent of cofilin phosphorylation are regulated by Ssh in response to changes in the levels and/or organisation of F-actin. Together with the recent finding that Ssh phosphatase activity is augmented by F-actin binding, these results identify Ssh-dependent regulation of phosphorylated cofilin levels as an important feedback control mechanism that maintains actin filament homeostasis during actin signalling.


Subject(s)
Actin Depolymerizing Factors/metabolism , Actins/metabolism , Homeostasis , Pseudopodia/metabolism , Actin Cytoskeleton/metabolism , Animals , Cells, Cultured , Drosophila Proteins/metabolism , Drosophila melanogaster , Humans , Kinetics , Mice , Phosphatidylinositol 3-Kinases/metabolism , Phosphoprotein Phosphatases , Phosphoric Monoester Hydrolases/metabolism , Phosphorylation , Signal Transduction
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