ABSTRACT
Irrigation is one of the most important uses of surface waters in the agricultural region of Vojvodina province (Serbia). The aim of the study was to assess the quality of water from Stara Tisa meander, based on the levels of pollution with metals, volatile compounds (VOC), pharmaceuticals, pesticides, and pathogenic bacteria, on sunflower, cabbage, cucumber, maize, barley, buckwheat, sorghum, radish, beans, and white mustard. Microbiological analysis was carried out using the dilution method and phytotoxicity assay according to ISTA filter paper method (germination energy (GE), germination (G), root and shoot length, fresh and dry weight). The sample was slightly contaminated with domestic, industrial, and agricultural xenobiotics and had low levels of nitrogen substances, metals, and organic micropollutants. Pesticides, metolachlor, tebuconazole, propiconazole, imidacloprid, and thiametoxam were detected at levels exceeding the maximum admissible concentrations (MACs), i.e., the sum value for neonicotinoids. The number of saprophytic (2.27 × 10(6) CFU mL(-1)) and coliform bacteria (5.33 × 10(2) CFU mL(-1)) was very high. The total number of sulphite reducing clostridia (10 cells mL(-1)) and Escherichia coli (5 cells mL(-1)) was very low. The GE and G of all tested plants, except sunflower, were not influenced by the total chemism of water sample. However, it inhibited root lengths of sunflower, cucumber, maize, and barley and stimulated shoot lengths of all species except maize and white mustard. These results indicate that it can be used for irrigation of cabbage and radish from the chemical point of view, but the microbiological traits should be considered prior to consumption since they are consumed raw. The overall results suggest that water from Stara Tisa should be purified before using for agricultural purposes.
Subject(s)
Agricultural Irrigation , Environmental Monitoring , Water Pollutants, Chemical/toxicity , Hordeum , Metals/analysis , Metals/toxicity , Pesticides/analysis , Pesticides/toxicity , Serbia , Wastewater/analysis , Wastewater/toxicity , Water Pollutants, Chemical/analysisABSTRACT
In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil.
Subject(s)
Crops, Agricultural/chemistry , Food Contamination , Glycine max/chemistry , Plants, Genetically Modified/chemistry , Seeds/chemistry , Soybean Oil/chemistry , Crops, Agricultural/drug effects , Crops, Agricultural/genetics , Crops, Agricultural/metabolism , DNA, Plant/isolation & purification , DNA, Plant/metabolism , Drug Resistance , Food Handling , Food Inspection/methods , Food Labeling/legislation & jurisprudence , Glycine/analogs & derivatives , Glycine/pharmacology , Herbicides/pharmacology , Plant Lectins/genetics , Plant Lectins/metabolism , Plants, Genetically Modified/drug effects , Plants, Genetically Modified/genetics , Plants, Genetically Modified/metabolism , Polymerase Chain Reaction , Real-Time Polymerase Chain Reaction , Reproducibility of Results , Seeds/drug effects , Seeds/genetics , Seeds/metabolism , Serbia , Soybean Proteins/genetics , Soybean Proteins/metabolism , Glycine max/drug effects , Glycine max/genetics , Glycine max/metabolism , GlyphosateABSTRACT
Genetically modified (GMO) rapeseed (Brassica napus) is not grown commercially in European Union, but several lines have been approved for production and use as food and feed. A case-specific monitoring of herbicide-tolerant rapeseed, events RT73, RF3 and T45 was established by Ministry of Agriculture of Republic of Serbia. The objectives of the present study were to introduce methods for detection of herbicide-tolerant GM oilseed rape, investigate occurrence and monitor the presence of GM rapeseed in seed and the feed products, as well as to develop a protocol for quantification. The study was based on 48 samples, rapeseed (33) and feed (15) products, imported from EU countries (Germany, Belgium, France, Czech Republic, Austria) and from domestic market. Seven positive feed samples and no positive seed samples have found. The percent of GMO in feed samples, estimated on semi-quantitative way, was below labelling threshold. Adventitious presence of GM materials in non-GM grain, derived food and feedstuffs is a concern to international grain trade and needs continuous monitoring.
