Microarthropod communities act as functional mediators of ecosystem recovery in abandoned metal(loid) mine tailings in semiarid areas.

Abandoned metal(loid) mine tailings show inhospitable conditions for the establishment of above- and below-ground communities (e.g., high metal(loid) levels, organic matter and nutrient deficiency). This worsens in semiarid areas due to the harsh climate conditions. Fertility islands (vegetation patches formed by plants that spontaneously colonize the tailings) can serve as potential nucleation spots fostering beneficial plant-microbial interactions. However, less attention has been paid to the soil invertebrates living beneath these patches and their functional role. Here, we studied whether the spontaneous plant colonization of abandoned metal(loid) mine tailings led to a greater presence of soil microarthropod communities and whether this could contribute to improving ecosystem functionality. Microarthropods were extracted, taxonomically identified and subsequently assigned to different functional groups (saphrophages, omnivores, predators) in bare soils and differently vegetated patches within metalliferous mine tailings and surrounding forests in southeast Spain. Microarthropod communities were significantly different in bare soils compared with vegetated patches in mine tailings and surrounding forests. Plant colonization led to an increase in microarthropod abundance in tailing soils, especially of mites and springtails. Moreover, saprophages and omnivores, but not predators, were favored in vegetated patches. The initial microarthropod colonization was mainly linked to higher organic matter accumulation and greater microbial activity in the vegetated patches within mine tailings. Moreover, soil formation processes already initiated in the tailings were beneficial for soil biota establishment. Thus, below-ground communities created an anchorage point for plant communities by primarily starting heterotrophic activities in the vegetated patches, thereby contributing to recover ecosystem functionality.

Low acclimation potential compromises the performance of water-stressed pine saplings under Mediterranean xeric conditions.

Predicted hotter and drier climatic conditions in the Mediterranean Basin will probably hamper current afforestations and reforestations by negatively influencing tree performance. Understanding how saplings can adjust their physiology to shortages in water availability is essential to predict early-stage success of forest ecological restoration. Pines are common target species used in afforestations and reforestations; however, the capacity of their saplings for physiological plasticity to promote drought tolerance remains largely unexplored. In this study, we evaluated the demographical and resource-use consequences of short-term irrigation among four pine species (Pinus halepensis, Pinus pinea, Pinus nigra and Pinus sylvestris) growing under water-limiting conditions in a common garden experiment. Summer irrigation increased the survival rate of those pines that were suffering from hydric stress under the xeric conditions of the common garden (i.e. P. pinea, P. nigra and P. sylvestris). Moreover, short-term water supplementation slightly enhanced aboveground biomass production across species. However, leaf isotopic composition and nutrient concentrations did not change after summer irrigation. Independently of water supplementation, P. halepensis was the best adapted species to water scarcity and showed the best physiological and growth performance. By contrast, P. pinea, P. nigra and P. sylvestris saplings exhibited drought-induced reductions in stomatal conductance and low water-use efficiency, nutrient deficiency, and severe N:P and N:K stoichiometric imbalances, leading to impaired growth. We conclude that the lack of physiological plasticity of water-stressed pine saplings to withstand the impacts of climate aridification will likely cause severe impairment of their nutrient status, growth and survival, with dire implications for the successful establishment of Mediterranean afforestation and reforestation programs.