Cytotoxic and genotoxic impact of TiO2 nanoparticles on A549 cells.

Titania nanoparticles are produced by tons, and included in commercial products, raising concerns about their potential impact on human health. This study relates their cytotoxic and genotoxic impact on a cell line representative of human lung, namely A549 alveolar epithelial cells.

In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-, genotoxicity and oxidative stress.

Silicon carbide (SiC) is considered a highly biocompatible material, consequently SiC nanoparticles (NPs) have been proposed for potential applications in diverse areas of technology. Since no toxicological data are available for these NPs, the aim of this study was to draw their global toxicological profile on A549 lung epithelial cells, using a battery of classical in vitro assays. Five SiC-NPs, with varying diameters and Si/C ratios were used, and we show that these SiC-NPs are internalized in cells where they cause a significant, though limited, cytotoxic effect. Cell redox status is deeply disturbed: SiC-NP exposure cause reactive oxygen species production, glutathione depletion and inactivation of some antioxidant enzymes: glutathione reductase, superoxide dismutase, but not catalase. Finally, the alkaline comet assay shows that SiC-NPs are genotoxic. Taken together, these data prove that SiC-NPs biocompatibility should be revisited.

In vitro assessment of thyroid and estrogenic endocrine disruptors in wastewater treatment plants, rivers and drinking water supplies in the greater Paris area (France).

The presence of estrogenomimetic compounds in the environment, and particularly in water resources, is well known. In contrast, little data is available about the disruption of the thyroid system, even though thyroid hormones are strongly involved in regulating metabolism, growth and development. The aim of this study was to carry out a parallel evaluation of the disruptions of thyroid and estrogenic hormone receptor transcriptional activities, induced by water samples from two wastewater treatment plants (WWTPs), in the river Seine, and from four drinking treatment plants located in the Paris area. Two in vitro bioassays were used for the evaluation of thyroid (PC-DR-LUC) and estrogenic (MELN) disruption. Our observations of thyroidal activity show that a disruption potential was only present in the WWTPs influents, whereas estrogenicity was systematically detected in both influents and effluents. The great majority of endocrine activity was removed during the biological process. In the river Seine, only estrogenicity was detected, and no activity was observed in drinking water supplies. Fractionation of the influents revealed that most of the thyroidal effect was associated with compounds with low polarity, and could be partly attributable to 4-nonylphenol.

A new bioluminescent cellular assay to measure the transcriptional effects of chemicals that modulate the alpha-1 thyroid hormone receptor.

Interactions of environmental pollutants with the thyroid endocrine axis have received much attention especially because thyroid hormones (THs) play a major role in mammalian brain development. In order to screen for compounds that act on the triiodothyronine (T3) signaling pathway, we developed a new reporter gene assay expressing luciferase under the control of the TH receptor (TR). PC12 cells expressing the alpha1-isoform of TR of avian origin were stably transfected with a luciferase gene controlled by the SV40 promoter, and enhanced by a four-spaced direct repeat (DR4) thyroid response element (TRE). The resulting PC-DR-LUC cells were used to optimize a T3 assay in multiwell microplates. This assay was highly sensitive (30 pM T3) and reproducible, and responded as expected to TH analogues. Several halogenated phenolic (3,3',5,5'-tetrabromobisphenol A, 3,3',5,5'-tetrachlorobisphenol A, 4-hydroxy-2',3,4',5,6'-pentachlorobiphenyl) and phenol (pentachlorophenol, 2,4,6-triiodophenol) compounds suspected of being thyroid-disrupting environmental chemicals induced partial agonistic and/or complex competitive/uncompetitive antagonistic responses in PC-DR-LUC cells at micromolar concentrations. A cell viability test indicated that these effects were not related to cytotoxicity of the chemicals. These results suggest that the PC-DR-LUC assay could be a valuable tool for the large-scale screening for thyroid receptor agonists and antagonists in vitro, and for detecting thyroid disruptors in the environment.

A comparison of an accelerated technique for casting post-and-core restorations with conventional techniques.

PURPOSE: This project compares an accelerated technique for the casting of post-and-core restorations with four traditional techniques. The accelerated technique uses two phosphate-bonded investments and the traditional techniques use a gypsum- and a phosphate-bonded investment. The study measures and compares the differences between the seating of the casting and the seating of the acrylic resin pattern. The effects of the techniques on the fit of castings with and without a ferrule are also compared. MATERIALS AND METHODS: Six groups of 10 castings were made from plastic patterns formed on a stainless steel test die. A different investment and/or burnout method was used for each group. Each group had two subgroups: ferruled and nonferruled. The fit of the plastic patterns was measured at two time intervals after forming, 2 weeks and 3 months. The patterns were invested immediately after the 3-month measurement, and the difference in fit of the castings was calculated. An ANOVA and Tuckey-Kramer test were done to determine the statistical validity. RESULTS: The seating of the patterns after 3 months of storage was consistently worse than the 2-week measurements of fit. The ferrule and nonferrule patterns were not statistically different in seating. Measurement of the castings showed that the ferruled castings seated significantly worse than the nonferrule castings. The difference in the seating of the castings as compared with the patterns was considered clinically unacceptable, showing a range of 0.301 mm to 0.528 mm. The nonferrule castings showed a significant difference in seating among groups. The difference ranged from -0.099 mm to 0.322 mm. CONCLUSIONS: The castings of the ferrule subgroups were considered clinically unacceptable and were not analyzed for significance. Among the nonferrule castings, the group using a gypsum investment and conventional technique for investing and burnout but no ring liner showed the best seating. The accelerated technique was intermediate in seating with a difference of 0.148 mm from the seating of the patterns. This group was significantly different from the two best groups but not from the remaining three groups.