Crambe meal and crude glycerin from biodiesel production in diets for finishing of crossbred cattle in feedlot.

Our hypothesis is that crambe meal can totally substitute soybean meal and crude glycerin can partially replace the corn in the diet without leading to losses of nutrient intake and productive performance. Two assays were performed using heifers and steers. In the first assay, 24 Beefalo-Nellore heifers with 18 months old with 311.5 ± 5.5 kg, and in the second assay, 24 Beefalo-Nellore steers with 18 months old with 317.0 ± 6.4 kg were evaluated. The experiment was carried out in a randomized complete design, in a 2 × 2 factorial scheme, consisting of two energy sources (corn and crude glycerin) and two protein sources (soybean meal and crambe meal). Both assays had 104 experimental days of feedlot. There was no interaction between the variation sources. There was an effect of protein source on nutrient intake for heifers except to neutral detergent fiber intake, which presented effect of energy source. Heifers fed soybean meal showed higher results for total weight gain and daily weight gain. There was observed effect of energy source on rib eye area and subcutaneous fat thickness. Steers fed soybean meal showed higher neutral detergent fiber intake and non-fibrous carbohydrates. Steers fed corn + crude glycerin showed higher results for total weight gain and daily weight gain. The steers fed soybean meal showed higher averages for subcutaneous fat thickness. Crambe meal and crude glycerin can be used as alternative sources of energy and protein to replace 100% of soybean and 50% of corn, respectively, for finishing Beefalo-Nellore cattle in a feedlot.

Combinations of by-products from biodiesel production included in the supplement for finishing heifers on deferred pastures.

We hypothesized that by-products from biodiesel production like crambe meal (Crambe abyssinica) totally replace soybean meal (Glycine max) and crude glycerin could partially replace corn (Zea mays). Our aim was to evaluate different supplements with combinations of crambe meal and crude glycerin replacing soybean meal or corn on performance and carcass traits of finishing heifers. Sixty Nellore heifers 18 months old were used, and 298.96 ± 21.99 kg of body weight allotted in Brachiaria brizantha pastures. All heifers were distributed in a randomized complete block design, with four treatments being the following supplements: corn + soybean meal (CS), corn + crambe meal (CCr), corn + soybean meal + crude glycerin (CSG), corn + crambe meal + crude glycerin + (CCrG). The animals were distributed in four paddocks of 13 hectares each, in a continuous grazing system. We evaluated pasture characteristics, intake, performance, carcass traits, characteristics, and centesimal composition of Longissimus thoracis muscle. There was an effect (P < 0.05) of inclusion of crambe meal on the performance of the animals for slaughter weight and daily gain. The supplement containing corn + soybean meal (CS) presented better conversion rates and food efficiency. There was an effect of inclusion of crambe meal and crude glycerin on the centesimal composition of Longissimus thoracis muscle. The use of crambe meal and crude glycerin as alternative sources from the agro-industry of biodiesel in concentrate supplement for finishing heifers in pastures can promote positive effects related to productive performance and carcass characteristics.

Biofuel by-product can replace partially corn in supplement for Angus × Nellore heifers: productive performance and meat characteristics.

The aim of this is study was to test the hypothesis that corn can be partially replaced by crude glycerin (at a level of 50%) without affecting performance, ingestive behavior, ruminal parameters, meat composition, and fatty acids profile on the meat of heifers. Sixty-four intact plus four rumen cannulated Angus × Nellore heifers (359.0 ± 12.15 kg BW) were used. The cannulated animals received the same diets as the intact ones to determine the ruminal N-NH3 concentration, pH, and chewing activity weekly. All heifers were maintained in Brachiaria brizantha pasture and supplemented with a concentrate containing corn, soybean meal, urea, premixed minerals, and glycerin partially replacing corn. A completely randomized experimental design was used, with two treatments (corn ground or glycerin + corn ground). The inclusion of crude glycerin (high fat) decreased nutrients intake and ruminal N-NH3 concentration in the rumen but increased lipid intake. Inclusion of glycerin had no effect (P > 0.05) on ruminal pH, daily gain, performance, time spent eating, rumination, idling, or water intake. The moisture, ash, and protein contents of meat were not affected (P > 0.05) by crude glycerin intake. Crude glycerin increased saturated fatty acids (C14:0, C16:0, and C18:0) and decreased unsaturated fatty acids (C18:2n6c and C20:1) in meat. Despite the reduction in nutrient intake, we recommend crude glycerin to replace corn in concentrate because glycerin did not impair the performance, meat composition, and ruminal pH. Therefore, crude glycerin is a by-product that can partially replace corn in diets efficiently.

Neuraminidase inhibitors rewire neutrophil function in murine sepsis and COVID-19 patient cells

Neutrophil overstimulation plays a crucial role in tissue damage during severe infections. Neuraminidase (NEU)-mediated cleavage of surface sialic acid has been demonstrated to regulate leukocyte responses. Here, we report that antiviral NEU inhibitors constrain host NEU activity, surface sialic acid release, ROS production, and NETs released by microbial-activated human neutrophils. In vivo, treatment with Oseltamivir results in infection control and host survival in peritonitis and pneumonia models of sepsis. Single-cell RNA sequencing re-analysis of publicly data sets of respiratory tract samples from critical COVID-19 patients revealed an overexpression of NEU1 in infiltrated neutrophils. Moreover, Oseltamivir or Zanamivir treatment of whole blood cells from severe COVID-19 patients reduces host NEU-mediated shedding of cell surface sialic acid and neutrophil overactivation. These findings suggest that neuraminidase inhibitors can serve as host-directed interventions to dampen neutrophil dysfunction in severe infections. At a GlanceIn a severe systemic inflammatory response, such as sepsis and COVID-19, neutrophils play a central role in organ damage. Thus, finding new ways to inhibit the exacerbated response of these cells is greatly needed. Here, we demonstrate that in vitro treatment of whole blood with the viral neuraminidase inhibitors Oseltamivir or Zanamivir, inhibits the activity of human neuraminidases as well as the exacerbated response of neutrophils. In experimental models of severe sepsis, oseltamivir decreased neutrophil activation and increased the survival rate of mice. Moreover, Oseltamivir or Zanamivir ex vivo treatment of whole blood cells from severe COVID-19 patients rewire neutrophil function.