Subject(s)
Hyaluronic Acid/adverse effects , Hyaluronoglucosaminidase/administration & dosage , Intraocular Pressure/drug effects , Ocular Hypertension/prevention & control , Animals , Anterior Chamber/drug effects , Drug Therapy, Combination , Injections , Ocular Hypertension/chemically induced , RabbitsABSTRACT
PURPOSE: To determine whether human trabecular meshwork cells (HTM) are a potential target tissue for thyroid hormone (3,3',5-triiodothyronine, T3). METHODS: Cultured HTM were assayed for the presence of thyroid hormone receptors (TRs) and retinoid X receptors (RXRs) by reverse-transcriptase polymerase chain reaction (RT-PCR) to detected TR and RXR mRNA, and by immunohistochemistry to detect nuclear TR and RXR proteins. Functionality of the TR was determined by analysis of 125I-T3 binding affinity and capacity in HTM nuclear extracts. Effects of T3 on modulation of hyaluronic acid (HA) levels in HTM were measured as a function of dose and duration of T3 administration. RESULTS: Analysis of RT-PCR and immunohistochemistry demonstrated that cultured HTM expressed TRalpha1, TRalpha2, and TRbeta1 but not TRbeta2; and RXRalpha but not RXRbeta and RXRgamma isoforms. Saturation binding and analysis of 125I-T3 to HTM nuclear extracts revealed Kd of 57 pM. The number of T3 binding sites extrapolated from a Scatchard plot was 7.3 x 10(10)/microg of HTM nuclear protein extract. T3 supplementation reduced the concentration of HA in the cell medium by 32-43% compared to cells grown in the absence of T3. CONCLUSIONS: Cultured HTM express three TR isoforms and one RXR isoform, bind T3 with an affinity similar to that of TR in responsive cells, and modulate their HA production in response to T3. These findings indicate that the human trabecular meshwork tissue has the capacity to respond to thyroid hormones.
Subject(s)
Receptors, Thyroid Hormone/metabolism , Trabecular Meshwork/metabolism , Adult , Cell Count/drug effects , Cells, Cultured/drug effects , DNA Primers/chemistry , Enzyme-Linked Immunosorbent Assay , Humans , Nuclear Proteins/drug effects , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , RNA, Messenger/analysis , Receptors, Retinoic Acid/drug effects , Receptors, Retinoic Acid/genetics , Receptors, Retinoic Acid/metabolism , Receptors, Thyroid Hormone/drug effects , Receptors, Thyroid Hormone/genetics , Retinoid X Receptors , Reverse Transcriptase Polymerase Chain Reaction , Trabecular Meshwork/cytology , Trabecular Meshwork/drug effects , Transcription Factors/drug effects , Transcription Factors/genetics , Transcription Factors/metabolism , Triiodothyronine/pharmacologyABSTRACT
PURPOSE: To determine the expression of CD44 isoforms in cultured human trabecular meshwork (HTM) and to discuss their possible relationship with outflow facility. METHODS: CD44 isoform expression in cultured HTM was qualitatively examined using immunohistochemistry and RT-PCR analysis. RESULTS: Immunohistochemistry of cultured HTM showed intense staining with a CD44s antibody, and with antibodies against CD44 exon 7, exon 11-12 and exon 14. By RT-PCR, at least three isoforms of CD44 were expressed in HTM: CD44s, CD44v-III and CD44v-I. CONCLUSIONS: At least three isoforms of CD44 are expressed in the HTM. CD44 may play a role in binding and turnover of hyaluronic acid in the trabecular meshwork, thereby regulating outflow facility.
Subject(s)
Hyaluronan Receptors/metabolism , Trabecular Meshwork/metabolism , Cells, Cultured , DNA Primers/chemistry , Exons , Humans , Hyaluronan Receptors/genetics , Immunoenzyme Techniques , Polymerase Chain Reaction , Trabecular Meshwork/cytology , Transcription, GeneticABSTRACT
To investigate whether the occasional increase in intraocular pressure that may arise following injection of sodium hyaluronan into the anterior segment during intraocular surgery is related to the polymer size of hyaluronan, controlled fragmentation of hyaluronan chains in vitro was obtained using progressive incubation with testicular hyaluronidase. The profile of molecular sizes of the hyaluronan polymers in various preparations was determined using molecular sieve column chromatography. Individual preparations were injected into six rabbit eyes and intraocular pressures were measured every one-half hour for 12 hours. Longer incubations of hyaluronan with hyaluronidase resulted in more extensive degradation with accumulation of shorter chain lengths. In the rabbit, mean intraocular pressure for 12 hours following intracameral injection of hyaluronic acid (HA) is proportional to the polymer size of HA. The occasional elevation of intraocular pressure that occurs following injection of hyaluronan during ophthalmic surgery can be avoided in part by assuring the rapid fragmentation of the large molecular size hyaluronan polymer.
Subject(s)
Hyaluronic Acid/chemistry , Hyaluronic Acid/pharmacology , Intraocular Pressure/drug effects , Ocular Hypertension/chemically induced , Animals , Anterior Eye Segment/drug effects , Chromatography, Gel , Hyaluronic Acid/isolation & purification , Hyaluronoglucosaminidase/pharmacology , Particle Size , RabbitsABSTRACT
The possible role of the human corneal endothelium in the turnover of anterior chamber hyaluronic acid (HA) was investigated. Hyaluronidase, an endoglycosidase that degrades HA and other glycosaminoglycans, is thought to play a role in HA homeostasis. The presence of hyaluronidase in the corneal endothelium was demonstrated immunohistochemically in sections from normal adult human cornea. Additionally, by using a modified enzyme-linked immunosorbent assay-like assay, active hyaluronidase was detected in the supernatant from primary culture human corneal endothelial cells. The optimal activity for the corneal endothelial hyaluronidase was in the acid range (pH 4.0), similar to previously isolated lysosomal hyaluronidase. Further immunohistochemistry showed that the corneal endothelial cells also express CD44, the receptor for HA, which would allow endocytosis of HA. Human corneal endothelial hyaluronidase may play a role in normal anterior segment HA metabolism and in the degradation of highly concentrated HA used as a visco-elastic.
Subject(s)
Anterior Chamber/metabolism , Endothelium, Corneal/enzymology , Hyaluronic Acid/metabolism , Hyaluronoglucosaminidase/physiology , Adult , Cell Culture Techniques , Endothelium, Corneal/cytology , Enzyme-Linked Immunosorbent Assay , Homeostasis/physiology , Humans , Hyaluronan Receptors/metabolism , Hydrogen-Ion Concentration , ImmunohistochemistryABSTRACT
PURPOSE: Hyaluronic acid (HA) is the predominant glycosaminoglycan (GAG) of the human vitreous. Interaction of this HA with vitreous collagen is important for maintaining gel structure. The mechanism of HA homeostasis in the vitreous is incompletely understood. The aim of this study was to determine whether hyaluronidase, an endoglycosidase that degrades HA, was present in human vitreous. METHODS: Vitreous samples were collected from post-mortem eye bank specimens and from non-hemorrhagic, non-inflamed biopsy specimens. Vitreous hyaluronidase was purified by a series of column chromatographic steps, and its activity was measured by an ELISA-like assay and by substrate gel electrophoresis through and HA-impregnated gel. The purified hyaluronidase was also analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and by Western blotting. RESULTS: Hyaluronidase activity was detected in vitreous samples from both post-mortem and biopsy specimens. The enzyme was most active at acid pH, but demonstrated significant activity at neutral pH. The partially purified enzyme migrated as a 59 kDa protein on SDS-PAGE, and a single band on Western blots. CONCLUSIONS: Hyaluronidase is present in the human vitreous. Thus, hyaluronidase may be involved in HA catabolism in the vitreous and may play a role in determining its gel structure.
Subject(s)
Hyaluronoglucosaminidase/isolation & purification , Vitreous Body/enzymology , Blotting, Western , Chromatography, Affinity , Collagen/metabolism , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Humans , Hyaluronic Acid/metabolism , Hyaluronoglucosaminidase/analysis , Hydrogen-Ion Concentration , Vitreous Body/chemistryABSTRACT
Germline epsilon (I epsilon) transcription is requisite for IgE switch recombination. I epsilon transcription is markedly increased by ligation of CD40 and/or by IL-4 stimulation. By contrast, we found previously that stimulation through CD30 inhibits I epsilon transcription in EBV-transformed B cell lines. To characterize the molecular mechanisms involved in these contradictory events, the promoter elements that are responsible for I epsilon transcriptional regulation were determined using stable CAT reporter gene constructs. The results define a 95 bp CD30 responsive element (CD30RE) located 5' of the previously defined CD40 responsive element (CD40RE) that resides within the same 95 bp fragment as the IL-4RE and ablates CD40L induced I epsilon promoter activity. However, IL-4 overrides the inhibitory effect of CD30L. These results define a CD30RE and provide further evidence for the complex regulation of I epsilon transcription by various members of the CD40L/TNF alpha family of molecules.
Subject(s)
Immunoglobulin E/immunology , Immunoglobulin E/metabolism , Ki-1 Antigen/immunology , Ki-1 Antigen/metabolism , Membrane Glycoproteins/antagonists & inhibitors , Membrane Glycoproteins/immunology , Promoter Regions, Genetic/immunology , Base Sequence , CD40 Ligand , Cell Line, Transformed , Humans , Interleukin-4/immunology , Interleukin-4/metabolism , Ligands , Membrane Glycoproteins/metabolism , Molecular Sequence Data , Transcription Factors/immunologyABSTRACT
Engagement of CD40 by its ligand induces transcription of unrearranged Ig heavy chain genes, an initial step in switch recombination. The following studies were undertaken to understand the molecular basis of this response. Co-culture of S19 cells expressing membrane-bound CD40 ligand (CD40L) encoded by recombinant baculovirus with EBV-transformed B cell lines induced germline transcription of the epsilon gene in the absence of cytokines. To identify a putative CD40 response element, a reporter construct consisting of the 777 bp of the 5' flank of the human l epsilon region linked to the chloramphenicol acetyl transferase (CAT) gene was stably transfected into B cell lines. Stimulation with either CD40L-expressing Sf9 cells or IL-4 induced CAT activity. Deletional analysis of this promoter region confirmed that an IL-4 response element was identified within a 63 bp segment 3' to the IL-4-responsive element that was responsive to CD40 ligation. These results indicate that the germline epsilon promoter contains a CD40 response element that is distinct from that accounting fro IL-4 responsiveness. Activity of this response element may explain the capacity of ligation of CD40 to induce germline epsilon transcripts in the absence of cytokines.
Subject(s)
CD40 Antigens/metabolism , Genes, Immunoglobulin , Immunoglobulin epsilon-Chains/genetics , Interleukin-4/pharmacology , Membrane Glycoproteins/pharmacology , Promoter Regions, Genetic , Animals , Base Sequence , CD40 Ligand , Cell Line, Transformed , Humans , Molecular Sequence Data , SpodopteraABSTRACT
To assess the potential of CD40 ligand (CD40L) and the related molecules CD27 ligand (CD27), CD30 ligand (CD30L), and membrane TNF-alpha to stimulate B cell responses, expression of these proteins in the baculovirus system was performed. Sf9 cells expressing these membrane molecules were cultured with normal human B cells and a variety of B cell lines to assess the functional outcome. The signal provided by CD40L promotes aggregation of B cells, stimulates vigorous proliferation, and induces germ-line transcription of downstream heavy chain constant region genes in the absence of cytokine costimulation. In contrast, CD27L, CD30L, and TNF-alpha had no effects on B cell proliferation. CD27L and TNF-alpha had no effect on the induction of germ-line transcripts, whereas CD30L consistently inhibited constitutive and CD40L-induced germ-line transcription of the epsilon gene by B cell lines that express CD30. These results demonstrate the various members of the TNF family exert specific effects on human B cell function, with CD40L and CD30L providing powerful, but opposing, effects on l epsilon transcription.
Subject(s)
B-Lymphocytes/physiology , Membrane Glycoproteins/pharmacology , Tumor Necrosis Factor-alpha/pharmacology , Antigens, CD/pharmacology , B-Lymphocytes/drug effects , Base Sequence , CD27 Ligand , CD30 Ligand , CD40 Ligand , Genetic Vectors , Histocompatibility Antigens Class II , Humans , Lymphocyte Activation/drug effects , Membrane Proteins/pharmacology , Molecular Sequence Data , Recombinant Proteins/pharmacologyABSTRACT
Stimulation of human B cells with mAb to CD40 in the presence of IL-4 induces proliferation and differentiation into Ig-secreting cells. To delineate the molecular events leading to Ig secretion after stimulation via the CD40 molecule, the induction of germ-line transcripts of Ig H chain isotypes was analyzed by polymerase chain reaction. The results document that costimulation with mAb to CD40 and IL-4 induces sterile transcription of all Ig H chain isotypes. Of importance, stimulation with mAb to CD40 without the addition of IL-4 induced germ-line transcription of most downstream isotypes, suggesting that this signal is sufficient to initiate the first step in switch recombination.