ABSTRACT
We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.
Subject(s)
Antineoplastic Agents/pharmacology , Flavones/pharmacology , Hyaluronic Acid/pharmacology , Polyethylene Glycols/pharmacology , Polyethyleneimine/pharmacology , Animals , Antineoplastic Agents/chemistry , Cell Proliferation/drug effects , Cell Survival/drug effects , Drug Screening Assays, Antitumor , Flavones/chemistry , HeLa Cells , Hep G2 Cells , Humans , Hyaluronic Acid/chemistry , Mice , Molecular Structure , Morpholines/chemistry , NIH 3T3 Cells , Nanogels , Particle Size , Polyethylene Glycols/chemistry , Polyethyleneimine/chemistry , Surface PropertiesABSTRACT
This paper describes the fabrication and evaluation of phase-change material (PCM) nanoparticles containing chlorin e6 (Ce6) and nanodiamonds (NDs) for photodynamic and photothermal approaches for tumor therapy, respectively. The temperature of the PCM nanoparticles containing NDs (ND/PCM, 0.5mg/mL in water) is increased to 45°C during laser exposure for 5min. The singlet oxygen generation intensity of PCM nanoparticles containing Ce6 and NDs (Ce6/ND/PCM) is gradually increased with respect to the laser exposure time. Also, the release of Ce6 from Ce6/ND/PCM can be controlled in an on-and-off manner using laser. Cell ablation tests reveal that Ce6/ND/PCM greatly ablates KB cells upon laser exposure, which is attributed to both the temperature increase in the media and singlet oxygen generation by the released Ce6. In an animal model, tumor volume is notably reduced over time after the intratumoral injection of Ce6/ND/PCM and subsequent laser exposure with a higher efficiency compared to ND/PCM. The Ce6/ND/PCM can be a promising nanomedicine for tumor therapy.
Subject(s)
Lasers , Nanoparticles/administration & dosage , Neoplasms/therapy , Phototherapy , Porphyrins/administration & dosage , Radiation-Sensitizing Agents/administration & dosage , Animals , Cell Line , Cell Survival/drug effects , Chlorophyllides , Humans , Mice, Inbred BALB C , Mice, Nude , Nanoparticles/chemistry , Neoplasms/pathology , Porphyrins/chemistry , Radiation-Sensitizing Agents/chemistry , Singlet Oxygen/chemistryABSTRACT
The use of injectable bulking agents is a feasible alternative procedure for conventional surgical therapy. In this study, poly(dimethylsiloxane) (PDMS) microspheres coated with polydopamine (PDA) were developed as a potential injection agent to prevent migration in vocal fold. Uniform PDMS microspheres are fabricated using a simple fluidic device and then coated with PDA. Cell attachment test reveals that the PDA-coated PDMS (PDA-PDMS) substrate favors cell adhesion and attachment. The injected PDA-PDMS microspheres persist without migration on reconstructed axial CT images, whereas, pristine PDMS locally migrates over a period of 12 weeks. The gross appearance of the implants retrieved at 4, 8, 12 and 34 weeks indicates that the PDA-PDMS group maintained their original position without significant migration until 34 weeks after injection. By contrast, there is diffuse local migration of the pristine PDMS group from 4 weeks after injection. The PDA-coated PDMS microspheres can potentially be used as easily injectable, non-absorbable filler without migration.
Subject(s)
Bivalvia , Coated Materials, Biocompatible , Dimethylpolysiloxanes/chemistry , Indoles , Microspheres , Polymers , Animals , Excipients , Injections, Subcutaneous , Male , Microscopy, Electron, Scanning , Rabbits , X-Ray MicrotomographyABSTRACT
This paper describes the design of alendronate-conjugated nanodiamonds (Alen-NDs) and evaluation of their feasibility for bone-targeted delivery. Alen-NDs exhibited a high affinity to hydroxyapatite (HAp, the mineral component of bone) due to the presence of Alen. Unlike NDs (without Alen), Alen-NDs were preferentially taken up by MC3T3-E1 osteoblast-like cells, compared to NIH3T3 and HepG2 cells, suggesting their cellular specificity. In addition, NDs itself increased ALP activity of MC3T3-E1 cells, compared to control group (osteogenic medium) and Alen-NDs exhibited more enhanced ALP activity. In addition, an in vivo study revealed that Alen-NDs effectively accumulated in bone tissues after intravenous tail vein injection. These results confirm the superior properties of Alen-NDs with advantages of high HAp affinity, specific uptake for MC3T3-E1 cells, positive synergistic effect for ALP activity, and in vivo bone targeting ability. The Alen-NDs can potentially be employed for osteoporosis treatment by delivering both NDs and Alen to bone tissue.
Subject(s)
Alendronate/administration & dosage , Bone Density Conservation Agents/administration & dosage , Drug Carriers/administration & dosage , Nanodiamonds/administration & dosage , Alendronate/chemistry , Alendronate/pharmacokinetics , Alkaline Phosphatase/metabolism , Animals , Bone Density Conservation Agents/chemistry , Bone Density Conservation Agents/pharmacokinetics , Bone and Bones/metabolism , Cell Line , Drug Carriers/chemistry , Drug Carriers/pharmacokinetics , Durapatite/chemistry , Female , Hep G2 Cells , Humans , Mice, Inbred BALB C , Mice, Nude , Nanodiamonds/chemistry , Osteoporosis/drug therapyABSTRACT
Based on oil-in-water emulsion, uniform poly(dimethylsiloxane) (PDMS) beads were prepared using a simple fluidic device and then modified with polydopamine (PDA) to improve cell attachment. The size of the PDMS beads could be easily tuned by changing the flow rates of the discontinuous and continuous phases, and PDMS concentration in oil phase. The PDA-coated PDMS beads exhibited a dark and rough surface, whereas the pristine PDMS beads had a clear and smooth surface. The PDA layer at the surface of the PDMS beads was found to provide a favorable environment for cell culture due to its hydrophilic property. The PDA-coated PDMS beads can potentially be employed as filler materials for tissue engineering.
