ABSTRACT
Chemical constituents in ethyl acetate and butanol fractions of ethanol extracts from Acorus tatarinowii were separated by column chromatography. Bufo skeletal muscle fatigue model was established to study the anti-fatigue activity of separated compounds. Five compounds were separated and identified by spectroscopic analysis as acoramone(1),cycloartenone(2),2,4,5-trimethoxyl-2'-butoxy-1,2-phenyl propandiol(3),5-hydroxymethyl furfural(4), and 5-butoxymethyl furfural(5). Compound 3 was a new compound, and compounds 2 and 5 were separated from this plant for the first time. Compound 4 exhibited a notable anti-fatigue activity.
Subject(s)
Animals , Acorus , Chemistry , Bufonidae , Fatigue , Drug Therapy , Muscle, Skeletal , Plant Extracts , Chemistry , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To determine the chemical constituents of Jatropha curcas.</p><p><b>METHOD</b>Chemical constituents were obtained using various chromatography methods including silica gel column chromatography and HPLC. The structures of isolated compounds were determined by spectroscopic methods including 1H-NMR, 13C-NMR and MS.</p><p><b>RESULT AND CONCLUSION</b>Fourteen phenolic compounds were obtained from the stems of J. curcas and their structures were identified to be 5,4'-dihydroxy-3, 7, 3'-trimethoxyflavone (1), 5, 3', 4'-trihydroxy-3,7-dimethoxyflavone (2), 3-O-methylquercetin (3), 5, 6, 7-trimethoxycoumarin (4), tomentin (5), isoscopoletin (6), omega-hydroxypropioquaiacone (7), coniferaldehyde (8), 3, 5-dihydroxy-4-methoxybenzaldehyde (9), vanillic acid (10), isovanillin (11), 4-hydroxybenzaldehyde (12), cimifugin (13) and (E)-3-hydroxy-5-methoxy-stilbene (14). Among them, compounds 1-4 and 6-14 were isolated from the genus of Jatropha for the first time.</p>
