ABSTRACT
Outer membrane vesicles (OMVs) are nano-sized spherical vehicles, with a size range between 20-250 nm. OMVs are spontaneously secreted from Gram-negative bacteria and formed by lipid bilayer membranes, comprising multiple parent bacteria-derived components including bacterial antigens, pathogen-associated molecular patterns, proteins and lipids. OMVs have shown multiple potentials for the treatment of various diseases, including cancer therapy and bacterial infection. In this review, the structure, composition and methods for isolating and characterizing of OMVs were introduced. The applications of OMVs for diseases therapy were summarized and future perspectives were discussed.
ABSTRACT
Objective To study the effects of puerarin-phospholipid complex on lymphatic transport absorption of puerarin microemulsion based on the puerarin-phospholipid complex microemulsion and conventional microemulsion prepared before. Methods The chylomicron flow blocking approach was used to block lymphatic transport of the microemulsion, and the concentration of puerarin in plasma was determined by HPLC. Bioavailability of puerarin inblocking groups and non-blocking groups were compared to calculate the proportion of puerarin absorbed by lymphatic transport. Results The lymphatic transport of puerarin-phospholipid complex microemulsion was higher than that of conventional microemulsion, and the lymphatic translocation ratio was 51.3% and 40.6%, respectively. The AUC0-12 of puerarin-phospholipid complex microemulsion non-blocking and blocking groups were (5.489 ± 1.599) μg•h/mL and (2.673 ± 1.153) μg•h/mL, respectively; And AUC0-12 of puerarin routine microemulsion non-blocking and blocking groups were (4.158 ± 1.160) μg•h/mL and (2.478 ± 1.352) μg•h/mL, respectively. Conclusion The lymphatic transport of puerarin-phospholipid complex microemulsion can increase lymphatic transport efficiency, and AUC0-12 of which were higher than those of conventional microemulsion group.
