ABSTRACT
Objective Calculus Bovis(CB)is a kind of valuable traditional Chinese medicine,which has been used in clinic for a long time.It has been shown to have significant anti-stroke,anti-inflammatory and anti-tumor effects.But its mechanism for treating Prostate cancer(PCa)remains unclear.The purpose of this study was to explore the target and mechanism of its action in the treatment of prostate cancer throμgh network pharmacology and in vitro and in vivo experiments.Methods The effective compounds of Calculus Bovis were collected by TCM pharmacology database and analysis platform(TCMSP).Search for potential compound targets in TCMSP.Search the Drμgbank,GeneCards,OMIM,PharmGkb,and TTD databases for disease targets associated with prost cancer.Disease and compound targets were integrated in the STRING database to construct their interaction network(PPI)to reveal the key targets of compound treatment for prostate cancer.In order to elucidate the mechanism of Calculus Bovis in the treatment of prostate cancer,GO functional enrichment and KEGG pathway enrichment analysis were conducted using Cytoscape software.The mechanism of treating prostate cancer with Calculus Bovis was studied in vitro and in vivo.Results A total of 11 compounds with anti-prostate cancer activity were identified.Oleanolic acid,ursolic acid,ergosterol,deoxycorticosterone,methylcholine and cholverdin were potential effective components.A total of 367 targets of Calculus Bovis compounds and 2152 targets of prostate cancer were found.The core targets of Calculus Bovis in the treatment of prostate cancer included TP53,STAT3,AKT1,HSP90AA1,ESR1,SRC,JUN,RELA,CCND1,CDKN1A,EGFR,AR,etc.The biological functions of Calculus Bovis mainly involve oxidative stress response,response to steroid hormones,cell response to chemical stress,peptide-serine modification and phosphorylation,and protein serine/threonine kinase activity.Calculus Bovis treatment of prostate cancer mainly involves PI3K-AKT signaling pathway,TNF signaling pathway,MAPK signaling pathway,etc.In vitro and in vivo experiments showed that Calculus Bovis promoted apoptosis of PC3 cells of prostate cancer by inhibiting PI3K-AKT signaling pathway.Conclusion Calculus Bovis has a therapeutic effect on prostate cancer,and its function is related to inhibiting PI3K-AKT signaling pathway and promoting apoptosis of cancer cells.
ABSTRACT
OBJECTIVE To explore the positive rate of intercellular adhesion (ica) operon and its production- polysaccharide intercellular adhesion(PIA) and the relationship between the rate and adhesion of Staphylococcus epidermidis. METHODS Amplifying icaA of ica operon by PCR and then detecting it by agarose electrophoresis, detecting PIA by Congo red agar (CRA) to detect its adhesive ability by infusion set. RESULTS The icaA positive rate of S.epidermidis was 51.5%; the PIA positive rate was 50%; there was significant difference between the clone numbers of PIA positives and negatives (P
ABSTRACT
0.05.Conclusion Using XT-2000i Automated Hematology Analyzer to test the reticulocyte the reproducibility is better,and the linearity can content well with clinic,the rate of carry contamination is low,the stability is better too.Compared XT-2000i with the manual methods,the relativity is better. It can replace the manual methods.
ABSTRACT
<p><b>OBJECTIVE</b>To determine the relationship between vitamin D receptor (VDR) gene polymorphism and osteoporosis in postmenopausal women.</p><p><b>METHODS</b>The polymerase chain reaction-restriction fragment length polymorphism was used to detect VDR genotype in 40 patients with osteoporosis and 21 healthy postmenopausal women.</p><p><b>RESULTS</b>In the patients with osteoporosis, the bb, Bb, and BB genotype accounted for 82.5%, 17.5% and 0, respectively; in healthy groups, they were 85.71%, 14.29% and 0, respectively (P>0.05).</p><p><b>CONCLUSION</b>Significant association between VDR genotype and osteoporosis in Chinese women was observed in this study.</p>
Subject(s)
Female , Humans , Middle Aged , DNA , Genetics , Metabolism , Deoxyribonucleases, Type II Site-Specific , Metabolism , Gene Frequency , Genotype , Osteoporosis, Postmenopausal , Genetics , Polymorphism, Genetic , Receptors, Calcitriol , GeneticsABSTRACT
Objective;Discussing clinical utilization of Automation Urine Analyszer in the testing Hydrothorax and Hydroperitonium. Methods:Testing 80 specimen got from our clinci and patient by using Uritest-200 equipemnt to get the results of PRO and SC, using UF - 100 to get the results of RBC,WBC,EC which can be compared with the results which got by handwork. Results:Comparison between Uritest-200 and handwork method, PRO and SG usex2 examination, the results have very siginficant differ( P
ABSTRACT
OBJECTIVE:To determine the resistance of clinically isolated bacteria to meropenem METHODS:The resistance of 150 clinically isolated strains to meropenem and several other antibiotics was detected by K-B method and the drug resistant spectra to E coli were compared between meropenem and imipenem RESULTS:The sensitivity of 130 clinical isolates to meropenem was 100% No cross resistant strains of E coli was found to meropenem and imipenem CONCLUSION:Meropenem is highly sensitive to the clinical isolates,and its antibacterial spectrum is similar to that of imipenem
ABSTRACT
Objective:To study the resistance and its tendency of E.coli to antibiotics was to serve the rational administration in clinic.Methods:WHONET5 was employed to analyze the drug sensitivities of 616 E.coli isolated from the clinical specimens from January,2004 to July,2006 in hospital.Results:The resistance rates of E.coli to Ampicillin,Piperacillin,Amoxicillin/cla,Trimethoprim/Sulfamethoxazole and Ciprofloxacin exceeded 60%,and they were about 50% to Gentamicin,Cefazolin and Cefuroxime.In addition,the resistant rates were20% infra to Piperacillin/tazobactam,Cefepime,Ceftazidime,Cefoxitin,Amikacin and Furantoin,and there was no resistance to Imipenem.The detection rates of ESBLs to E.coli was 27.96%,32.68%,35.12% in 2004,2005,2006 respectively.Conclusions:The resistance rates of E.coli to Penicillins,Piperacillins/Clavulanic acid,Sulfanilamide,Quinolone,Gentamicin,the first and second generations Cephalosporins were high,and they were not suitable for clinical use.To Piperacillins/tazobactam,fourth generation Cephalosporins,Cephamycins,Imipenem,Amikacin and Furantoin,the resistance rates were relatively low,and they could be used clinically.The third generation Cephalosporins should be used cautiously due to its feasibility of inducing ESBLs even though its resistant rates were low.The production rate of ESBLs of E.coli ascended year by year.
