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1.
Forensic Sci Int ; 209(1-3): e11-5, 2011 Jun 15.
Article in English | MEDLINE | ID: mdl-21497467

ABSTRACT

An 88-year-old woman committed suicide by drinking a toxic amount of highly concentrated alcohol and setting two rooms of her flat on fire. As there was not enough oxygen, the fire went out, however. At autopsy, no thermal lesions were found on the body, but soot depositions in the airways and a COHb value of 14% pointed to the inhalation of fire fumes. The ethanol concentration in femoral blood was 6.62 per mille. The gastric mucosa was fixed by the ingested alcohol and showed hardly any autolytic changes despite a post-mortem interval of five days. Congener analysis of the gastric contents and the femoral blood indicated the uptake of a fruit distillate or its foreshot.


Subject(s)
Central Nervous System Depressants/poisoning , Ethanol/poisoning , Fires , Suicide , 1-Butanol/analysis , 1-Propanol/analysis , Aged, 80 and over , Butanols/analysis , Butanones/analysis , Carboxyhemoglobin/analysis , Central Nervous System Depressants/blood , Ethanol/blood , Female , Forensic Pathology , Forensic Toxicology , Gastric Mucosa/pathology , Gastrointestinal Contents , Humans , Larynx/pathology , Methanol/analysis , Pentanols/analysis , Pharynx/pathology , Smoke Inhalation Injury/pathology , Soot
2.
Genome ; 53(9): 698-709, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20924419

ABSTRACT

Brachiaria humidicola (Rendle) Schweick. is a warm-season grass commonly used as forage in the tropics. Accessions of this species were collected in eastern Africa and massively introduced into South America in the 1980s. Several of these accessions form a germplasm collection at the Brazilian Agricultural Research Corporation. However, apomixis, ploidy, and limited knowledge of the genetic basis of this germplasm collection have constrained breeding activities. The objectives of this work were to identify genetic variability in the Brazilian B. humidicola germplasm collection using microsatellite markers and to compare the results with information on the following: (1) collection sites of the accessions; (2) reproductive mode and ploidy levels; and (3) genetic diversity revealed by morphological traits. The evaluated germplasm population is highly structured into four major groups. The sole sexual accession did not group with any of the clusters. Genetic dissimilarities did not correlate with either geographic distances or genetic distances inferred from morphological descriptors. Additionally, the genetic structure identified in this collection did not correspond to differences in ploidy level. Alleles exclusive to either sexual or apomictic accessions were identified, suggesting that further evaluation of the association of these loci with apospory should be carried out.


Subject(s)
Brachiaria/classification , Brachiaria/genetics , Genetic Variation , Microsatellite Repeats , Africa , Alleles , Brachiaria/anatomy & histology , Brachiaria/physiology , Brazil , Cytogenetic Analysis , DNA, Plant/genetics , Evolution, Molecular , Genes, Plant , Genetic Markers , Genome, Plant , Genotype , Geography , Phenotype , Phylogeny , Ploidies , Polymerase Chain Reaction , Polymorphism, Genetic , Polyploidy , Reproductive Physiological Phenomena/genetics , Seeds
3.
Mol Ecol Resour ; 10(3): 576-9, 2010 May.
Article in English | MEDLINE | ID: mdl-21565062

ABSTRACT

This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.

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