Automatic Inference of Rat's Hindlimb Trajectory to Synchronize with Forelimb Gait Through Phase.

Synchronous forelimb-hindlimb gait pattern is important to facilitate natural walking behavior of an injured rat with total transection. Since our ultimate research goal is to build a rehabilitation robotic system to simulate the natural walking pattern for spinalized rats, this research aims to address an immediate goal of automating the inference of the rat's hindlimb trajectory from its own forelimb movement. Our proposed method uses unsupervised learning to extract independent forelimb and hinblimb phases. From the phase information, a relationship between forelimb and hindlimb trajectory can then be calculated. Results show that the proposed method has the potential to be used in a rehabilitation robotic system.

Exploring new treatment for spinalized rats by synergising robotic rehabilitation system and regenerative medicine.

Spinal cord injury (SCI) is a traumatic event which leads to the loss of sensory and motor functions of the body. Complete recovery of these functions are usually limited due to the inability of the damaged axons within the central nervous system (CNS) to regenerate autonomously. Here, a combinatorial regenerative and rehabilitative approach to regrow damaged axons was proposed. Sprague-Dawley rats were subjected to a severe T9-T10 full tranection injury with a 2mm gap. Neurotrophin-3 (NT-3) loaded fibrous scaffold was implanted within the gap to provide topographical guidance for the axons to cross the injured region. To study the effect of rehabilitation, the rats were separated into 2 groups; those that undergo rehabilitation (trained, N=4) and those that do not undergo rehabilitation (untrained, N=3). In order to rehabilitate the rats, a rehabilitation robotic system consisting of a body weight support, hindlimb manipulator, and treadmill was developed. Preliminary results showed that rats which underwent rehabilitation had more robust axonal regeneration within the scaffold after 1 month. However, the Basso, Beattie, and Bresnahan (BBB) score, which is an indicator of locomotor recovery, do not show much significance between trained and untrained rats.