ABSTRACT
Refining nutraceutical conjugated metal nanoparticles (NPs) and understanding their interactions with the cellular micro-environment is necessary for their application in nanomedicine. In the present experiment, we studied the effect of quercetin functionalized gold nanoparticles (AuQurNP) on skin fibroblast and keratinocyte cell migration. Spherical shaped AuQurNPs of 47 nm in size were formed due to the interaction of hydroxyl and carbonyl groups of quercetin with Au atoms as revealed by incremental algorithm-based analysis. AuQurNP containing up to 5 µg l-1 of Au with quercetin (5.2 ± 1.6 ng ml-1) was least toxic to fibroblasts. AuQurNP effectively reduced the generation of intracellular ROS (up to 63%) through free-radical scavenging activity. AuQurNP also enhanced the rate of migration of fibroblasts (24 h) and keratinocytes (20 h) in artificially created wounds. The rate of migration of the cells towards the wound edge was in the order of AuQurNP > control > quercetin > AuNP. AuQurNP also significantly increased the expression of TGFß1 protein, thereby inducing the downstream SMAD complex (SMAD 2-4). Downregulation of the inhibitory protein SMAD 7 by AuQurNP helped in the nuclear translocation of SMADs 3 and 4. Collectively, the present in vitro study demonstrates the action of AuQurNP on the SMAD family and the interconnected molecular mechanism leading to the cell migration process.
ABSTRACT
Carotid intima media thickness (CIMT) is used commonly as a non-invasive test for assessment of degree of atherosclerosis. The present study, in a tertiary care centre of Eastern India was aimed to determine the relation between CIMT and the known and novel risk factors of ischemic stroke. Of the 62 patients studied, 82% were hypertensive and 61% were smokers, 95% were above 50 years of age. Seventy one percent of our patients had high CIMT. CIMT was signifi cantly related to high sensitivity C-reactive protein (hsCRP), fi brinogen, LDL levels and age of the patient. By logistic regression analysis, hsCRP, fi brinogen and age were signifi cantly associated with high CIMT. When the patients have elevation of both hsCRP and fi brinogen, the risk of high CIMT were even higher. Our study highlights the needs for better screening in high risk patients.
ABSTRACT
Background: The 20-metre multistage shuttle run test is a useful method for the regular monitoring of aerobic fitness. However, the validity of the test should be established in the particular population prior to application. The aim of the study was to validate the applicability of the 20-metre multistage shuttle run test in non-athlete, girls from Kolkata, India. Methods: Thirty-six untrained girls from different schools in Kolkata (age range 14–16 years) were recruited for the study. Direct estimation of cardiorespiratory endurance (VO2max) comprised treadmill exercise followed by expired gas analysis using Scholander micro-gas analyser. VO2max was indirectly predicted by the 20-metre multistage shuttle run test. Results: The difference between the mean (SD) VO2max values of the direct measurement, 32.91 (2.66) ml.kg-1.min-1, and the 20-metre multistage shuttle run test, 33.79 (2.56) ml.kg-1.min-1, was statistically significant (P < 0.01). However, limits of agreement analysis suggested that the 20-metre multistage shuttle run test can be applied for use with the studied population. Intra-class correlation coefficients also suggested good reliability of the 20-metre multistage shuttle run data. Conclusion: The results suggest that the use of the 20-metre multistage shuttle run test for the prediction of VO2max is justified in the studied population. For better prediction of VO2max, a new equation has been developed based on the present data for untrained girls from Kolkata.
