Pyeloureteral junction stenosis and ureteral valve causing hydronephrosis.

A young female patient underwent ureteral resection and Hynes-Anderson pyeloureteroplasty because of pyeloureteral junction stenosis and a ureteral valve (UV), which caused hydronephrosis. Despite using ultrasonography and intravenous urography the ureteral valve was observed only on exploration. UV should be considered as a possible cause of upper urinary tract obstruction; retrograde urography can be essential.

Capability for reactive gliosis develops prenatally in the diencephalon but not in the cortex of rats.

In this study, the glial reactions to stab wounds were investigated on a large population of newborn (P0) and fetal rats, by the immunohistochemical staining of the glial fibrillary acidic protein. The lesions penetrated both the cortex and the diencephalon. The fetuses were lesioned in utero from the 17th embryonic day (E17) and were born on E22 or E23 in the natural way. In the cortex usually no reactive gliosis developed although definitive tissue destructions remained after the lesion. Weak and incomplete glial reactions were observed in a few cases of E20 or P0 lesions only. In the diencephalon, however, the same stabbings provoked massive glial reactions. The timing and the morphology of this reaction were similar to those found in adult animals. At E17 the lesion did not result in reactive gliosis even in the diencephalon. Our study highlights two phenomena: (i) depending on the brain area servere glial reactions can already follow fetal lesions, and (ii) the appearance of the capability for glial reactions may be a stage of the local tissue maturation in every brain area and cannot be considered as a function of brain development in general. Probably, the capability for glial reactions can take place only when certain histogenetic processes (e.g., cell migration, axon growth, apoptosis) have been at least mostly accomplished, but which of the local development events are the determining ones remains to be investigated.

A rapid replacement of vimentin-containing radial glia by glial fibrillary acidic protein-containing astrocytes in transplanted telencephalon.

The present study follows the early events in the development of astroglia in rat embryonic (E15) tissue grafted into the cortex of adult rats. Astroglial elements (radial glia and astrocytes) were studied by glial fibrillary acidic protein (GFAP) and vimentin immunohistochemistry on post-transplantation (PT) days 7, 11, 14, 17, and 21. At PT7, GFAP-immunopositive elements were only scarce fibers in the transplants. At PT11, a dense network of long, GFAP-immunopositive fibers enmeshed the entire transplant, and astrocytes were already recognized. The fibers also showed vimentin immunoreactivity. By PT14, astrocytes became the predominant GFAP-labeled elements, although a few long fibers persisted. When compared with in situ development, the grafts showed earlier GFAP-immunoreactivity and earlier appearance of astrocytes, as well as a more rapid transition from the immature to the mature form of the glial system.