Skeletal muscle protease activities in the early growth and development of wild Atlantic salmon (Salmo salar L.).

Growth-related dynamics of intracellular protease activities in four year classes of the Atlantic salmon (Salmo salar L. 1758) parr and smolts inhabiting salmon rivers of northwestern Russia (the White Sea basin) were studied. Cathepsin B, cathepsin D, proteasome, and calpain activities in the skeletal muscles of salmon were assessed to investigate their relative contribution to the total protein degradation as well as to young fish growth process. It was confirmed that calpain activity dominates in salmon muscles while proteasome plays a minor role, in contrast to terrestrial vertebrates. Calpain and proteasome activities were maximal at the early post-larval stage (in parrs 0+) and declined with age (parrs 1+ through 2+) dropping to the lowest level in salmon smolts. Annual growth increments and proteolytic activities of calpains and proteasome in the muscles of salmon juveniles changed with age in an orchestrated manner, while lysosomal cathepsin activities increased with age. Comparing protease activities and growth increments in salmon parr and smolts we suggested that the partial suppression of the protein degradation could be a mechanism stimulating efficient growth in smoltifying salmon. Growth and smoltification-related dynamics of protease activities was quite similar in salmon populations from studied spawning rivers, such as Varzuga and Indera; however, some habitat-related differences were observed. Growth increments and protease activities varied in salmon parr 0+ (but not on later ages) inhabiting either main rivers or small tributaries apparently due to habitat difference on the resources for fish growth.

Biochemical markers of pollutant responses in macrozoobenthos from the White Sea: intracellular proteolysis.

Coastal environments of Kandalaksha Gulf in the White Sea (Russia) despite nature conservation efforts are heavily influenced by human activities. Biological effects of complex environmental pollution, including organic substances, heavy metals, and oil hydrocarbons, were assessed in widely distributed marine invertebrates, Gammarus duebeni (Crustacea, Amphipoda) and Mytilus edulis (Mollusca, Bivalvia), collected from a series of anthropogenically-impacted areas and distanced reference sites in Kandalaksha Gulf. The parameters of intracellular protein degradation pathways such as cytosol calpain system and lysosomal cathepsins B (CatB) and cathepsin D (CatD) were studied. The response reactions observed in invertebrates vary in specificity and ranged from adaptive to destructive depending on the total contaminant level and the nature of predominant pollutant. The ecological relevance of studied parameters as biomarkers was confirmed by their ability to indicate both expose to pollutants and adverse effects at the organism level.