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1.
Nitric Oxide ; 87: 31-42, 2019 06 01.
Article in English | MEDLINE | ID: mdl-30862476

ABSTRACT

The gaseous mediators nitric oxide (NO), carbon monoxide (CO) and lately also hydrogen sulfide (H2S) have been described to contribute to the interplay of protein type- and lipid mediators in the regulation of wound healing. In particular, the recently reported role of H2S in skin repair remains largely unresolved. Therefore we assessed the expressional kinetics of potential H2S-producing enzymes during undisturbed skin repair: the cystathionine-γ-lyase (CSE), the cystathionine-ß-synthase (CBS) and the 3-mercaptopyruvate sulfurtransferase (MPST). All three enzymes were not transcriptionally induced upon wounding and remained silent through the acute inflammatory and proliferative phase of skin repair. By contrast, CSE expression started to increase significantly at the later stages of healing, when cellular proliferation ceases within the granulation tissue and neoepidermis. The importance of H2S production in late healing phases was supported by a strong induction of otherwise not-induced CBS to complement the loss of CSE function in CSE-deficient mice. Immunohistochemistry revealed hair follicle keratinocytes and basal keratinocytes of the neo-epidermis covering the wound area as sources of CSE expression. Subsequent in vitro studies implicated a role of CSE-derived H2S for keratinocyte differentiation: the H2S-donor GYY4137 markedly increased the Ca2+-triggered expression of the early keratinocyte differentiation markers cytokeratin 10 (CK10) and involucrin (IVN) in cultured human keratinocytes. Here, GYY4137-derived H2S strongly enhanced CK10 expression by increasing the binding of RNA polymerase II to the CK10 promoter.


Subject(s)
Cystathionine gamma-Lyase/metabolism , Hydrogen Sulfide/metabolism , Keratin-10/metabolism , Keratinocytes/metabolism , Wound Healing/physiology , Animals , Cystathionine gamma-Lyase/genetics , Female , Humans , Mice, Inbred C57BL , RNA Polymerase II/metabolism , Skin/pathology , TATA Box , Wounds and Injuries/pathology
2.
J Mol Med (Berl) ; 95(3): 257-271, 2017 03.
Article in English | MEDLINE | ID: mdl-28054119

ABSTRACT

Renal mesangial cells are regarded as main players in glomerular inflammatory diseases. To investigate a possible crosstalk between inflammatory and hypoxia-driven signaling processes, we stimulated cultured mouse mesangial cells with different inflammatory agents and analyzed the expression of prolyl hydroxylase domain containing proteins (PHDs), the main regulators of hypoxia-inducible factor (HIF) stability. Administration of IL-1ß (1 nM) and TNF-α (1 nM), a combination further referred to as cytokine mix (CM), resulted in a fivefold increase in PHD3 but not PHD1 and PHD2 mRNA expression compared to untreated controls. In contrast, a combination of IL-1ß, TNF-α with lipopolysaccharide (10 µg/ml), and interferon-γ (20 ng/ml) designated as CM+ showed a high (60-fold) induction of PHD3 and a moderate (twofold) induction of PHD2 mRNA expression. Interestingly, CM+ but not CM induced the expression of inducible NO synthase and endogenously produced NO was responsible for the immense induction of PHD3 in mesangial cells treated with CM+. We found that CM+ affected PHD3 expression mainly via the NO/HIF axis, whereas PHD3 regulation by CM occurred in a NF-κB-dependent manner. In turn, silencing of PHD3 expression resulted in a decrease in the mRNA expression of ICAM-1, MIP-2, MCP-1, and CXCL-10, which are under control of NF-κB. In a rat model of mesangio-proliferative glomerulonephritis, PHD3 mRNA and protein expression was markedly induced and this effect was nearly abolished when rats were treated with the iNOS-specific inhibitor L-NIL, thus confirming our findings also in vivo. KEY MESSAGE: PHD3 expression induced by cytokines is NF-κB dependent in mesangial cells. Endogenously produced NO further augments PHD3 expression via HIF-1α. PHD3 expression is induced by NO in anti-Thy-1 glomerulonephritis.


Subject(s)
Glomerulonephritis/genetics , Nitric Oxide/immunology , Procollagen-Proline Dioxygenase/genetics , Up-Regulation , Animals , Cells, Cultured , Glomerulonephritis/immunology , Glomerulonephritis/pathology , Humans , Interleukin-1beta/immunology , Mesangial Cells/immunology , Mesangial Cells/metabolism , Mesangial Cells/pathology , Mice , Mice, Inbred C57BL , NF-kappa B/immunology , Procollagen-Proline Dioxygenase/immunology , RNA, Messenger/genetics , Tumor Necrosis Factor-alpha/immunology
3.
J Cereb Blood Flow Metab ; 37(8): 3015-3026, 2017 Aug.
Article in English | MEDLINE | ID: mdl-27909266

ABSTRACT

Despite the efficacy of neuroprotective approaches in animal models of stroke, their translation has so far failed from bench to bedside. One reason is presumed to be a low quality of preclinical study design, leading to bias and a low a priori power. In this study, we propose that the key read-out of experimental stroke studies, the volume of the ischemic damage as commonly measured by free-handed planimetry of TTC-stained brain sections, is subject to an unrecognized low inter-rater and test-retest reliability with strong implications for statistical power and bias. As an alternative approach, we suggest a simple, open-source, software-assisted method, taking advantage of automatic-thresholding techniques. The validity and the improvement of reliability by an automated method to tMCAO infarct volumetry are demonstrated. In addition, we show the probable consequences of increased reliability for precision, p-values, effect inflation, and power calculation, exemplified by a systematic analysis of experimental stroke studies published in the year 2015. Our study reveals an underappreciated quality problem in translational stroke research and suggests that software-assisted infarct volumetry might help to improve reproducibility and therefore the robustness of bench to bedside translation.


Subject(s)
Brain Infarction/diagnostic imaging , Brain/diagnostic imaging , Image Processing, Computer-Assisted/methods , Ischemic Attack, Transient/diagnostic imaging , Software , Animals , Brain/blood supply , Brain Infarction/etiology , Disease Models, Animal , Ischemic Attack, Transient/complications , Male , Mice, Inbred C57BL , Reproducibility of Results , Sensitivity and Specificity , Translational Research, Biomedical
4.
Sci Rep ; 6: 29808, 2016 07 14.
Article in English | MEDLINE | ID: mdl-27411966

ABSTRACT

H2S is an important signalling molecule involved in diverse biological processes. It mediates the formation of cysteine persulfides (R-S-SH), which affect the activity of target proteins. Like thiols, persulfides show reactivity towards electrophiles and behave similarly to other cysteine modifications in a biotin switch assay. In this manuscript, we report on qPerS-SID a mass spectrometry-based method allowing the isolation of persulfide containing peptides in the mammalian proteome. With this method, we demonstrated that H2S donors differ in their efficacy to induce persulfides in HEK293 cells. Furthermore, data analysis revealed that persulfide formation affects all subcellular compartments and various cellular processes. Negatively charged amino acids appeared more frequently adjacent to cysteines forming persulfides. We confirmed our proteomic data using pyruvate kinase M2 as a model protein and showed that several cysteine residues are prone to persulfide formation finally leading to its inactivation. Taken together, the site-specific identification of persulfides on a proteome scale can help to identify target proteins involved in H2S signalling and enlightens the biology of H2S and its releasing agents.


Subject(s)
Peptides/chemistry , Proteome/chemistry , Proteomics/methods , Sulfides/chemistry , Tandem Mass Spectrometry/methods , Amino Acid Sequence , Chromatography, Liquid/methods , Cysteine/analogs & derivatives , Cysteine/chemistry , Cysteine/metabolism , Disulfides/chemistry , Disulfides/metabolism , HEK293 Cells , Humans , Hydrogen Sulfide/chemistry , Hydrogen Sulfide/metabolism , Peptides/metabolism , Proteome/metabolism , Sulfides/metabolism
5.
Brain Res ; 1624: 380-389, 2015 Oct 22.
Article in English | MEDLINE | ID: mdl-26271715

ABSTRACT

Accumulating lines of evidence indicate that hydrogen sulfide (H2S) contributes to the processing of chronic pain. However, the sources of H2S production in the nociceptive system are poorly understood. Here we investigated the expression of the H2S releasing enzyme cystathionine γ-lyase (CSE) in the nociceptive system and characterized its role in chronic pain signaling using CSE deficient mice. We show that paw inflammation and peripheral nerve injury led to upregulation of CSE expression in dorsal root ganglia. However, conditional knockout mice lacking CSE in sensory neurons as well as global CSE knockout mice demonstrated normal pain behaviors in inflammatory and neuropathic pain models as compared to WT littermates. Thus, our results suggest that CSE is not critically involved in chronic pain signaling in mice and that sources different from CSE mediate the pain relevant effects of H2S.


Subject(s)
Cystathionine gamma-Lyase/metabolism , Ganglia, Spinal/metabolism , Hydrogen Sulfide/metabolism , Inflammation/metabolism , Neuralgia/metabolism , Animals , Cystathionine gamma-Lyase/genetics , Disease Models, Animal , Formaldehyde/toxicity , Gene Expression Regulation/genetics , Hyperalgesia/etiology , Hyperalgesia/metabolism , Inflammation/chemically induced , Inflammation/pathology , Male , Mice , Mice, Inbred C57BL , Mice, Transgenic , Motor Activity/physiology , Neuralgia/pathology , Pain Measurement , Spinal Cord/metabolism , Up-Regulation , Zymosan/pharmacology
6.
Biochem Pharmacol ; 93(3): 362-9, 2015 Feb 01.
Article in English | MEDLINE | ID: mdl-25437456

ABSTRACT

Glomerular mesangial cells are smooth muscle cell-like pericytes and are regarded as key players in kidney diseases. In an inflammatory setting, these cells produce high amounts of inflammatory cytokines, chemokines and redox mediators such as reactive oxygen species or nitric oxide (NO). The temporal production of ROS, NO and other redox mediators markedly contributes to the final outcome of inflammatory diseases. Recently, we reported that platelet-derived growth factor forced mesangial cells to activate the regulatory subunit of protein kinase A (PKA RI) by a redox-dependent mechanism but independent from changes in cyclic AMP. This prompted us to further analyze the dimerization of PKA RI and activation of PKA-driven signalling in an inflammatory context. Stimulation of rat mesangial cells with interleukin-1ß and tumour necrosis factor-α [2 nM] induced the formation of PKA RI heterodimers in a time-dependent manner. PKA RI dimerization was accompanied with the formation of ROS, NO and peroxynitrite as well as a depletion of reduced glutathione. Furthermore, dimerization of PKA RI was paralleled by enhanced activity of PKA as shown by the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) at serine 157 that was independent of the formation of cyclic AMP. Remarkably, exogenously administered peroxynitrite potently induced dimerization of PKA RI, whereas pharmacologic inhibition of inducible NO synthase (iNOS) and scavenging of peroxynitrite reduced PKA RI dimerization and VASP phosphorylation to control levels thus clearly indicating a causal role for endogenously formed peroxynitrite on PKA signalling. Consequently, the treatment of inflammatory diseases with anti-oxidants or NOS inhibitors may alter PKA activity.


Subject(s)
Cyclic AMP-Dependent Protein Kinases/biosynthesis , Cytokines/pharmacology , Kidney/drug effects , Kidney/enzymology , Mesangial Cells/drug effects , Mesangial Cells/enzymology , Signal Transduction/drug effects , Animals , Cells, Cultured , Enzyme Induction/drug effects , Enzyme Induction/physiology , Oxidation-Reduction/drug effects , Rats , Signal Transduction/physiology
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