ABSTRACT
248 respiratory specimens were examined using by Nested-PCR and by conventional methods (solid and liquid media). 54 specimens were culture-positive for M. tuberculosis, 51 of these were detected by PCR and conventional methods, whereas 3 were missed by PCR and 15 were only positive by PCR. 12 of the 15 culture-negative specimens derived from patients under treatment for tuberculosis. The 3 specimens, which are PCR negative could also be detected after modification of the method. The very high sensitivity depends on the high positive rate (21.8%) of the investigated specimens, so it is necessary to look also for other than pulmonary specimens, but it seems that his Nested-PCR is a highly specific and sensitive method for detecting of M. tuberculosis.
Subject(s)
Mycobacterium Infections, Nontuberculous/diagnosis , Mycobacterium avium-intracellulare Infection/diagnosis , Mycobacterium bovis/isolation & purification , Mycobacterium tuberculosis/isolation & purification , Polymerase Chain Reaction/methods , Tuberculosis, Pulmonary/diagnosis , Bronchoalveolar Lavage Fluid/microbiology , Humans , Mycobacterium Infections, Nontuberculous/microbiology , Mycobacterium avium Complex/isolation & purification , Mycobacterium avium-intracellulare Infection/microbiology , Nontuberculous Mycobacteria/isolation & purification , Sensitivity and Specificity , Sputum/microbiology , Tuberculosis, Pulmonary/microbiologyABSTRACT
A patient with an immediate type hypersensitivity reaction against poppy seed is reported. Clinical symptoms consisted of swelling of the oral mucosa, vomiting, respiratory distress, and urticaria. Specific IgE antibodies were demonstrable by RAST.
Subject(s)
Food Hypersensitivity/etiology , Papaver , Plants, Medicinal , Seeds , Adult , Female , Humans , Radioallergosorbent TestABSTRACT
Follicular hyperkeratosis was observed in a patient with multiple myeloma. This keratosis is considered to be a cutaneous manifestation of multiple myeloma, since similar cases have been observed before. In addition, the patient had cutaneous, ocular, and articular signs and symptoms of cryocrystalglobulinemia, ie, cutaneous vasculitis, blurring, and joint swellings.
Subject(s)
Cryoglobulinemia/complications , Darier Disease/complications , Multiple Myeloma/complications , Adult , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Cryoglobulinemia/pathology , Darier Disease/pathology , Humans , Male , Melphalan/administration & dosage , Multiple Myeloma/drug therapy , Prednisone/administration & dosage , SyndromeABSTRACT
We report two cases with ichthyosis vulgaris, hypogenitalism and hypogonadism. So far, little endocrinological information has been available on this association and the exact type of ichthyosis was unknown. Our first patient suffered from very severe hypergonadotropic hypogonadism, whereas the second patient showed normal levels of luteinizing hormone, but slightly elevated follicle stimulating hormone values. In lipoprotein electrophoresis we found fast moving beta-lipoproteins in the first patient and a normal electrophoretic mobility of pre- beta and beta-lipoproteins in the second patient. Correspondingly, steroid sulfatase (STS) testing revealed STS deficiency in the first patient and normal STS activity in the second patient, thus excluding X-linked recessive ichthyosis. These two different types in the association of ichthyosis with hypogenitalism and hypogonadism could not be discriminated by clinical, morphological and cytogenetic studies.
Subject(s)
Hypogonadism/genetics , Ichthyosis/genetics , Lipoproteins/blood , Sulfatases/deficiency , Abnormalities, Multiple/genetics , Adult , Genitalia, Male/abnormalities , Genotype , Hormones/blood , Humans , Hypogonadism/metabolism , Ichthyosis/metabolism , Limb Deformities, Congenital , Male , Pedigree , Steryl-Sulfatase , Sulfatases/analysisABSTRACT
The intrinsic clotting, the kinin generating and the fibrinolytic systems were investigated in 10 patients with hereditary angioedema (HAE), 10 patients with chronic urticaria and 18 healthy volunteers. In spite of the fact that patients suffering from HAE severely lack C1 INH, neither the intrinsic coagulation nor the fibrinolytic systems are impaired. There was a slight decrease of plasma kallikrein--as already known--and moreover a greater decrease in HMW-kininogen, and increase in Factor XII levels. Furthermore, activation of pre-kallikrein was delayed in these patients. These findings make it apparent that lowered HMW-kininogen levels compensate the lack of C1 INH, thus preventing an enhanced activation of the intrinsic clotting and the fibrinolytic systems.
Subject(s)
Angioedema/genetics , Blood Coagulation , Complement C1 Inactivator Proteins/analysis , Angioedema/blood , Chronic Disease , Complement C4/analysis , Fibrinolysis , Humans , Prekallikrein/analysis , Urticaria/blood , Urticaria/immunologyABSTRACT
Following an acute febrile infection of the respiratory tract, a 35-year-old man suffered from livid and painful discoloration of his acral regions even after moderate cold exposure. In his serum, high titers of cold agglutinins with anti-I specificity were present. Cold-related symptoms disappeared within 3 weeks. The clinical features and the finding of anti-I cold agglutinins are compatible with postinfections cold agglutinin disease following an infection with mycoplasma pneumoniae. However, antibodies directed against mycoplasma pneumoniae were only demonstrable after the cold agglutinin-dependent symptoms had disappeared.
Subject(s)
Agglutinins/immunology , Antibodies, Bacterial/immunology , Mycoplasma pneumoniae/immunology , Pneumonia, Mycoplasma/immunology , Adult , Cryoglobulins , Humans , Male , Skin/pathologyABSTRACT
A functional determination of C1 esterase inhibitor (C1 INH) can be easily performed with an amidolytic assay by monitoring the inactivation of plasma kallikrein. In patients with urticaria as well as in healthy donors kallikrein inactivation, determined as the ratio of kallikrein activity at t60/t15, was found to be 0.47 +/- 0.06; in patients with hereditary angioneurotic edema (HANE). However, it amounted to 0.80 +/- 0.06. There was a good correlation between the inactivation rate of kallikrein and the protein levels of C1 INH (r = -0.93, p less than 0.001). In patients with HANE, levels of plasma kallikrein were slightly decreased (mean = 0.37 +/- 0.11 U/ml, normal mean = 0.47 +/- 0.09 U/ml), but still sufficient for monitoring kallikrein inactivation. In the case of one patient with functionally inactive C1 INH protein, the inactivation of kallikrein was impaired as in the conventional form of the disease.
Subject(s)
Complement C1 Inactivator Proteins/analysis , Angioedema/blood , Angioedema/genetics , Humans , Hydrogen-Ion Concentration , Kallikreins/metabolism , Kinetics , Urticaria/blood , alpha-Macroglobulins/analysisABSTRACT
In man, apolipoprotein A-IV is characterized by a genetically determined polymorphism controlled by two codominant alleles. Two isoforms of this apolipoprotein, designated A-IV-1 and A-IV-2, can be identified by isoelectric focusing. Among 1000 healthy factory workers participating in an epidemiological study, A-IV-1 (genotype 1-1) was observed in 85%; A-IV-2 (genotype 2-2), in 0.5%; and A-IV-1 in combination with A-IV-2 (genotype 1-2), in 14%. In four nonrelated subjects, an apolipoprotein A-IV variant (A-IV-Münster), characterized by a slightly more basic isoelectric focusing behavior than A-IV-2, was detected in combination either with A-IV-1 or A-IV-2. Mendelian inheritance of this variant could be demonstrated.
Subject(s)
Apolipoproteins A , Apolipoproteins/genetics , Polymorphism, Genetic , Humans , Isoelectric Focusing , PhenotypeABSTRACT
A case with partial lipodystrophy and osteomyelitis is presented. Low C3 levels due to C3 nephritic factor may have contributed to the development of osteomyelitis.
Subject(s)
Lipodystrophy/complications , Osteomyelitis/complications , Adolescent , Complement C3 Nephritic Factor/metabolism , Complement C3 Nephritic Factor/physiology , Female , Humans , Immunoelectrophoresis , Lipodystrophy/blood , Lipodystrophy/immunology , Osteomyelitis/etiologyABSTRACT
The chemoattractant efficiencies of a Propionibacterium acnes (P. acnes) cell wall preparation, a P. acnes culture supernatant, and a soluble comedonal extract in the presence and absence of autologous serum for polymorphonuclear leukocytes (PMNs) have been compared in the present study. It has been found that all three preparations have no or very little chemotactic activity for PMNs in the absence of serum. In the presence of autologous serum chemotactic factors is generated by all preparations via the alternative complement pathway. The relative efficiencies of the various preparations to induce chemotactic factor by the alternate complement pathway has been evaluated. Based on the bacterial numbers of the original preparations from which the test preparations had been derived the comedonal extract appears to be more efficient in generating chemotactic factor than the other preparations. It is concluded that in vivo generation of chemotactic factors occurs mainly via the alternate complement pathway activated by soluble comedonal factors diffusing through the follicular wall.
Subject(s)
Acne Vulgaris/blood , Chemotactic Factors/pharmacology , Chemotaxis, Leukocyte , Neutrophils/physiology , Propionibacterium acnes , Skin , Tissue Extracts/pharmacology , Acne Vulgaris/immunology , Blood , Complement Pathway, Alternative , Culture Media , Culture Techniques/methods , Humans , InflammationABSTRACT
A monoclonal gammopathy was observed in three patients with long-term and widespread scleredema (Buschke's disease). There was no evidence of multiple myeloma in any patient. Deposition of monoclonal immunoglobulins in the skin was not detected by direct immunofluorescence microscopy. In contrast to scleromyxedema (lichen myxedematosus), from which scleredema can be distinguished clinically and histologically, the monoclonal immunoglobulins in two cases were of IgG2-kappa and IgG3-kappa type. Only one of the three patients had IgG1-lambda paraproteinemia, which is frequently seen in scleromyxedema. Our findings suggest that diffuse scleredema may be characterized by paraproteinemia but that the possible role of monoclonal immunoglobulins in the pathogenesis of this disease has yet to be resolved.
Subject(s)
Immunoglobulin Heavy Chains/analysis , Immunoglobulin Light Chains/analysis , Immunoglobulin gamma-Chains/analysis , Immunoglobulin kappa-Chains/analysis , Paraproteinemias/complications , Scleredema Adultorum/complications , Adult , Aged , Female , Humans , Immunoelectrophoresis , Male , Middle Aged , Paraproteinemias/diagnosis , Scleredema Adultorum/pathology , Skin/pathologyABSTRACT
Clinical and laboratory findings of four patients with Sézary syndrome and monoclonal gammopathy are presented. In the literature five cases with the association of these symptoms have been reported. Among the authors' series of 13 patients with Sézary syndrome, monoclonal gammopathy was observed far more often than could be expected from its natural incidence. It appears likely that monoclonal gammopathy is related to Sézary syndrome.
Subject(s)
Hypergammaglobulinemia/complications , Sezary Syndrome/complications , Aged , Female , Humans , Hypergammaglobulinemia/immunology , Immunoglobulin A/analysis , Immunoglobulin M/analysis , Male , Middle Aged , Sezary Syndrome/blood , Sezary Syndrome/pathology , Skin/ultrastructureABSTRACT
Hypotheses concerning phenotypic males with a female karyotype (46,XX males) are discussed. This peculiar chromosomal anomaly may be explained either by a mutation of autosomal sex determining genes or by a translocation of Y chromosomal genes to an X chromosome or an autosome. Recent studies concerning the H-Y antigen indicate that mosaicism with cell lines containing a Y chromosome may not be made responsible for phenotypic males with a 46,XX karyotype.
Subject(s)
Sex Chromosome Aberrations/genetics , Chromosome Banding , Female , HLA Antigens/genetics , Humans , Male , Mosaicism , X Chromosome , XYY Karyotype/genetics , Y ChromosomeABSTRACT
Mycosis fungoides was observed in a 71-year-old male with Mediterranean lymphoma, a B-cell malignancy. It is proposed that this association is not incidental since hypergammaglobulinaemia and even monoclonal gammopathies have repeatedly been described in cutaneous T-cell lymphomas. Mediterranean lymphoma might have resulted from (a) helper cell activities of tumor T-lymphocytes, (b) common antigenic stimuli, or (c) deranged T-B cooperation due to concomitant mycosis fungoides.