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1.
FEBS Lett ; 238(2): 307-14, 1988 Oct 10.
Article in English | MEDLINE | ID: mdl-2901990

ABSTRACT

The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the catalytic domain shows very clear homology to E. coli aminopeptidase N and contains Zn2+ ligands. Therefore these residues are part of the active site. However, no homology of the anchor/junctional peptide domain is found suggesting that the juxta- and intra-membraneous parts of the molecule have been added/preserved during development. It is speculated that this part carries the apical address.


Subject(s)
Aminopeptidases , DNA , Intestines/enzymology , Amino Acid Sequence , Aminopeptidases/genetics , Animals , Base Sequence , CD13 Antigens , Catalysis , Cloning, Molecular , Codon , DNA/genetics , Escherichia coli/enzymology , Escherichia coli/genetics , Humans , Molecular Sequence Data , Nucleic Acid Hybridization , Protein Biosynthesis , RNA, Messenger/genetics , Rabbits , Sequence Homology, Nucleic Acid , Swine
2.
J Virol ; 20(1): 14-21, 1976 Oct.
Article in English | MEDLINE | ID: mdl-978789

ABSTRACT

Disruption of purified lymphocytic choriomeningitis (LCM) virus with Nonidet P-40 in 0.5 M KCl followed by sucrose gradient centrifugation in 0.3 M KCl led to the isolation of two viral nucleoproteins (RNPs) as well as 40S and 60S ribosomal subunits. The largest viral RNP sedimented heterogenously at 123S to 148S and was associated with 23S and 31S viral RNA. The other viral RNP sedimented at 83S and was associated with 23S viral RNA. The buoyant density in CsCl was determined to be 1.32 g/cm3 for the viral RNP. Densities of 1.52 and 1.60 g/cm3 were determined for the 40S and 60S subunits, similar to those of the BHK-21 cells subunits dissociated by 0.5 M KCl. The viral RNPs were partly sensitive to RNase.


Subject(s)
Lymphocytic choriomeningitis virus/ultrastructure , RNA, Viral/analysis , Ribosomes/analysis , Viral Proteins/analysis , Centrifugation, Density Gradient , Ribonucleases/metabolism
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