ABSTRACT
In the present study an experimental rat model is presented enabling analysis of nerve regeneration in vivo through preformed tissue holes. The tissue space allows to bridge a gap about 10 mm. The neovascularization could follow the physiological course using the way through the epi- and perineurium in the chamber. The regeneration rate was investigated with and without application of a mixture of gangliosides showing a significant difference between both groups. Gangliosides stimulated the axonal outgrowth and the restoration of well-vascularized nerve fascicles. Further investigations should examine the effects of different neurotrophic substances in the tube.
Subject(s)
Gangliosides/pharmacology , Heparin/pharmacology , Muscles/innervation , Nerve Regeneration/drug effects , Sciatic Nerve/drug effects , Animals , Female , Nerve Degeneration/drug effects , Nerve Degeneration/physiology , Nerve Regeneration/physiology , Rats , Rats, Inbred Strains , Sciatic Nerve/physiologyABSTRACT
Described in this paper is a new artificial chamber model to enable in vivo investigation of nerve fiber regeneration. Transection of the sciatic nerve of a rat was followed by analysis of axonal regeneration and revascularisation in the tissue chamber. Vascularisation of the endoneurium via the newly formed epineurium and perineurium was almost physiological in the cavity formed. The latter was found to be suitable for application of drugs and neurotrophic substances.
