[Stimulation of interferon formation by natural and synthetic inducers in cultures of human tonsil cells]. / Stimuliatsiia interferonoobrazovaniia prirodnymi i sinteticheskimi induktorami v kul'turakh kletok mindalin cheloveka.

Conditions of interferon production stimulation were studied in human tonsillar cell cultures exposed to natural and synthetic inducers : poly(I) . poly(C), phage f2 RNA replicase, phytohemagglutinin and the low-molecular inducer gossypol (beta-aminoethyl sulfoxide Na). It has been shown that being inferior in the productivity per one cell to the continuous lymphoblastoid Raji and Namalva cell liness the tonsillar cell cultures, due to their high density, produce rather high interferon titers reaching hundreds of IU50/ml. The viability of the tonsillar cell cultures and their incubation at 37 degrees C during 24 hours are rather important for adequate interferon production.

[Viral interaction with stimulated and nonstimulated human tonsil cells]. / Vzaimodeistvie virusov so stimulirovannymi i nestimulirovannymi kletkami mindalin cheloveka.

Interferon induction and the course of infection with paramyxovirus (Newcastle disease virus) and alphaviruses (Venezuelan equine encephalomyelitis, Sindbis, Semliki forest viruses) in human tonsillar cell cultures were studied in comparison with continuous Burkitt lymphoma, Raji, and Nawalva cells. The virus inducers stimulated production of dozens and hundreds IU50/ml of interferon in tonsillar cell cultures. No virus replication could be detected either in intact or phytohemagglutinin-stimulated tonsillar cells. Phytohemagglutinin treatment may lead to blocking of interferon production induced by Newcastle disease virus.