[The activity of redox-regulatory systems in the primary and recurrence tumors in vulvar cancer]. / Aktivnost' redoks-reguliruiushchikh sistem v pervichnykh i retsidivnykh opukholiakh vul'vy.

The activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), the content of reduced glutathione (GSH) and malondialdehyde (MDA) were investigated in the samples of the tumor, peritumoral zone and healthy tissue, taken at the line of resection, were obtained from 14 patients with primary squamous cell carcinoma of the vulva, and 13 patients with local recurrence appeared in the period from 3 months to 7 years. by conventional spectrophotometric methods. The content of GSH and the activity of SOD, GPx, GR, GST were significantly increased, while MDA was decreased in the tissue of the primary carcinoma of the vulva in compared with the healthy tissue. Differences in the functioning of the investigated system of enzymes in the peritumoral zone were also revealed in the primary and recurrent tumoral process. Similar but much less pronounced changes were also observed in the recurrent tumor. It is suggested that such dynamics of activity of the studied system with the progression of cancer process can be the result of adaptation to changes in the local biochemical status of healthy (nonmalignant) tissue of the organ carrying the tumor and reflect the metabolic features of the recurrent tumor.

[Effect of iron nanoparticles on free radical oxidation process in blood of rats with Pliss lymphosarcoma]. / Vliianie nanochastits zheleza na pokazateli svobodnoradikal'nogo okisleniia v krovi krys s limfosarkomoi Plissa.

The use of metal nanoparticles (NPs) for cancer treatment requires careful examination of their biological effects. The aim of this study was to determine parameters of oxidative processes in the blood of tumor-bearing animals treated with metallic iron NPs only. The markers of antioxidant status and accumulation of lipid peroxidation products were measured in erythrocytes and blood plasma of rats with Pliss lymphosarcoma (PLS) and intact rats. PLS animals were treated eight times with iron NPs (at a dose of 1.25 mg/kg bw (main group), rats of the control group received saline (0.3 ml). In control animals, an increase in malondialdehyde (MDA) was observed in red blood cells (RBC) by 45%; this was accompanied by compensatory increase in reduced glutathione (GSH) and catalase by 24% and 14.3%, respectively (p<0.05). In plasma an increase in MDA by 167.4% (p<0.01) and a decrease in oxidase activity of ceruloplasmin (CP) by 36.8% (p<0.001) were found. In the main group there was a decrease of accumulation of lipid peroxidation products in the blood. Intensity of detected changes depended on the antitumor effect: rats with growing LSP showed a tendency to the decrease in the RBC MDA level and normalization of plasma MDA; in animals with LSP regression this marker did not differ from normal values. In all animals of the main group the CP content was basically the same as in intact rats while GSH increased in the group without therapeutic effect (by 218.6%) and in the group with the effect by 69% (versus normal values; p<0.01). SOD activity in the rats with LSP growth significantly increased (by 42%), in the rats with regression decreased (by 30%) with subsequent normalization. Thus, administration of iron NPs caused activation of the antioxidant system in blood and a significant decrease in the manifestations of oxidative stress associated with tumor growth.

[The level of endogenous intoxication iт oncologic gynecologic patients.]

The leading role of syndrome of endogenous intoxication in structure of clinical manifestations of tumor disease conditions actuality of studying components determining its development, in dynamics of malignant process and under anti-tumor therapy. Among all studied groups of oncogynecological patients, the most expressed decreasing of functional activity of albumin binding toxic compounds is observed under ascitic form of ovary cancer. In the group of patients with ovary cancer of stage I-II and also in condition of remission of process in patients with ovary cancer of stage III, the values of total and effective concentration of albumin and its binding capacity, on the contrary, are approaching to donor values. Under cervical carcinoma and vulva cancer the statistically significant decreasing of effective concentration of albumin and its binding capacity was established. However, thisdecreasing is manifested in minor degree than in patients with ascitic form of ovary cancer.

[Determining the relative content of cisplatin in cells by acridine orange fluorescence intensity].

The fluorescence intensity of acridine orange (AO) dye bound to living human leukocytes, rat leukocytes, and Pliss lymphosarcoma cells of rat has been studied by fluorescence microscopy. The cell suspension of each test was divided into two parts, to one of which the drug cisplatin was added to a final concentration of 0.1 µg/mL and the mixture was incubated for 20 - 60 min. Acridine orange was then added and the average intensity of red and green fluorescence of cells was determined. The brightness of AO in the green region of the spectrum was reduced approximately 3-fold (p = 0.001) in cells with cisplatin. In the red region of the fluorescence, differences in the brightness of cells with and without cisplatin were not observed. These data indicate that a decrease in the brightness of green fluorescence of AO can be a test for the presence of cisplatin in cells.

Experimental study of structural, functional, and biochemical changes in immune organs under conditions of antitumor activity of copper nanoparticles.

The effects of copper nanoparticles on the structure and function of the immune system organs (thymus and spleen) and intensity of free radical processes in the spleens of rats with sarcoma 45 were studied. A relationship between morphological and biochemical changes and antitumor efficiency of copper nanoparticles was demonstrated.