ABSTRACT
The present study was undertaken to evaluate the contraceptive efficacy of methanol extract of Dendrophthoe falcata Ettingsh (family-Loranthaceae), stem in male albino rats as reported in folk remedies. Adult proven fertile male rats were gavaged methanol extract of D. falcata stem at 50, 100 and 200mg/rat/day for 60 days. The activity was compared with standard drug, i.e. Lonidamine. On day 61 the animals were autopsied and the testes, epididymides, seminal vesicle and ventral prostate were dissected out and weighed. Sperm motility and density and serum testosterone level were assessed. The sperm motility and density were significantly reduced. The histoarchitecture of testes revealed degenerative changes in the seminiferous tubules, arrest of spermatogenesis at the stage of round spermatid. Serum testosterone levels were decreased significantly in all treatment groups. It is concluded that D. falcata methanol stem extract showed a significant effect on fertility in male rats as reported in folk remedies.
Subject(s)
Contraceptive Agents, Male/pharmacology , Loranthaceae , Animals , Dose-Response Relationship, Drug , Genitalia, Male/cytology , Genitalia, Male/drug effects , Indazoles/pharmacology , Male , Methanol , Organ Size/drug effects , Plant Extracts/pharmacology , Plants, Medicinal , Rats , Solvents , Spermatozoa/cytology , Spermatozoa/drug effects , Spermatozoa/physiology , Testosterone/bloodABSTRACT
The present study was undertaken to evaluate the effect of fluoride toxicity on the reproductive system of male rats. Sexually mature male Wistar rats were exposed to 2, 4, and 6 ppm sodium fluoride in their drinking water for 6 months ad libitum. Sperm motility and density in cauda epididymis were assessed. Biochemical and histological analysis were performed in reproductive organs. Fluoride treatment brought about a significant decrease in the weight of testis, epididymis, and ventral prostate. The sperm motility and density were significantly reduced. There was a marked reduction in the number of primary spermatocyte, secondary spermatocyte, and spermatids. The Sertoli cell counts and their cross sectional surface areas were significantly decreased. The Leydig cell nuclear area and the number of mature Leydig cells were also significantly decreased. The protein content of the testis and epididymis were significantly reduced. Fructose in the seminal vesicles and cholesterol in testes were increased significantly. In conclusion, sodium fluoride administrated in drinking water of 2, 4, and 6 ppm concentration for 6 months to male rats adversely affected their fertility and reproductive system.
Subject(s)
Genitalia, Male/drug effects , Sodium Fluoride/toxicity , Animals , Cell Count , Cholesterol/metabolism , Fructose/metabolism , Genitalia, Male/metabolism , Genitalia, Male/pathology , Leydig Cells/drug effects , Male , Organ Size/drug effects , Proteins/drug effects , Proteins/metabolism , Rats , Rats, Wistar , Sperm Count , Sperm Motility/drug effects , Spermatids/drug effects , Spermatocytes/drug effectsABSTRACT
In spite of the considerable development in contraceptive technology, search for male antifertility agents in plants continues to be a potential area of investigation. Many plants have been known to possess antifertility activity, but limited attempts have been made to scientifically evaluate these claims. Hence the purpose of this study was to evaluate the antifertility and reproductive toxicity potential of Dendrophthoe falcata (Loranthaceae) in male Wistar rats. An oral 70% methanolic extract of stem of D. falcata at a dose level of 100 mg/kg wt/day fed to male albino rats for 60 days did not decrease body weight, while the testes and epididymides were significantly reduced, and the seminal vesicles and ventral prostate also showed a significant reduction (P < 0.01). Treated animals showed a notable depression of spermatogenesis. As a result of 100 mg/kg extract feeding, the preleptotene spermatocytes, secondary spermatocytes, step-19 spermatids and the mature Leydig cells decreased by 74.36%, 80.03%, 79.87%, 32.37%, respectively. At this dose Leydig cell nuclear area and cytoplasmic area, as well as the cross sectional surface area of Sertoli cells, were significantly reduced (P < 0.001) when compared to controls. The reduced sperm count and motility resulted in 100% negative fertility at 100 mg/kg dose level. A significant fall in the total protein and sialic acid content in the testes, epididymides, seminal vesicle and ventral prostate, as well as in the glycogen content of testes was also observed. The level of serum protein, cholesterol, billirubin, SGOT, SGPT, blood urea, and hematological indices were unaltered. In conclusion, Dendrophthoe falcata brought about the inhibition of spermatogenesis.
Subject(s)
Antispermatogenic Agents/pharmacology , Contraceptive Agents, Male/pharmacology , Fertility/drug effects , Loranthaceae , Spermatogenesis/drug effects , Animals , Dose-Response Relationship, Drug , Male , Plant Stems/chemistry , Plants, Medicinal , Rats , Rats, WistarABSTRACT
Methanolic extract of Albizia lebbeck bark when administered orally at the dose level of 100 mg/rat/day to male rats of proven fertility for 60 days did not cause any significant loss in their body weights but the weights of reproductive organs, i.e. testis, epididymides, seminal vesicle and ventral prostate were decreased in a significant manner when compared to controls. Sperm motility as well as sperm density were reduced significantly which resulted in reduction of male fertility by 100%. Marked decline in the germ cell population was noticed. Population of preleptotene, pachytene, secondary spermatocytes and step-19 spermatid were declined by 60.86%, 65.81%, 71.56% and 66.55%, respectively. Cross-sectional surface area of sertoli cells as well as the cells counts were found to be depleted significantly. Leydig cells nuclear area and number of mature Leydig cells were decreased by 60.03% and 51.56%, respectively. Serum testosterone levels showed significant reduction after A. lebbeck extract feeding. Oral administration of the extract did not affect red blood cell (RBC) and white blood cell (WBC) count, haemoglobin, haematocrit and glucose in the blood and cholesterol, protein, triglyceride and phospholipid in the serum. In conclusion, A. lebbeck bark extract administration arrests spermatogenesis in male rats without noticeable side effects.
Subject(s)
Albizzia , Androgen Antagonists/pharmacology , Antispermatogenic Agents/pharmacology , Fertility/drug effects , Administration, Oral , Albizzia/chemistry , Androgen Antagonists/administration & dosage , Androgen Antagonists/isolation & purification , Animal Structures/drug effects , Animals , Antispermatogenic Agents/administration & dosage , Antispermatogenic Agents/isolation & purification , Body Weight/drug effects , Genitalia, Male/drug effects , Germ Cells/drug effects , Male , Methanol/chemistry , Organ Size/drug effects , Plant Bark/chemistry , Plant Extracts/administration & dosage , Plant Extracts/pharmacology , Rats , Rats, Wistar , Sperm Count , Sperm Motility/drug effects , Testosterone/bloodABSTRACT
The present study was undertaken to evaluate the antifertility activity of the active principle, i.e. lupeol acetate, isolated from benzene extract of Alstonia scholaris in male albino rats. The treatment with lupeol acetate at the dose level of 10 mg/rat/day did not cause any significant change in the body weights, but significant reduction in the weight of reproductive organs, i.e. testes, epididymides, seminal vesicle and ventral prostate, was observed. Testicular sperm count, epididymal sperm count and motility were found significantly declined when compared with controls, which resulted in reduction of male fertility by 100%. Arrest of spermatogenesis was noted at various stages with production of primary spermatocytes (preleptotene and pachytene), secondary spermatocytes and step-19 spermatids were decreased by 52.36, 54.91, 55.67 and 69.65%, respectively. The seminiferous tubules appeared reduced in size by 24.62%. Cross-sectional surface area of Sertoli cells as well as their counts were found to be significantly depleted. Leydig cell nuclear area and number of mature Leydig cells were decreased by 27.65 and 35.47%. Biochemical parameters of tissues i.e. protein, sialic acid, glycogen and cholesterol content of testes and seminal vesicular fructose also showed significant reduction.
Subject(s)
Alstonia/chemistry , Fertility/drug effects , Plant Extracts/toxicity , Spermatogenesis/drug effects , Triterpenes/toxicity , Animals , Cell Count , Genitalia, Male/drug effects , Genitalia, Male/pathology , Male , Organ Size/drug effects , Pentacyclic Triterpenes , Rats , Rats, Wistar , Sperm Motility/drug effectsABSTRACT
AIM: To evaluate the antifertility activity of the methanolic pod extract of Albizzia lebbeck (L.) Benth in male albino rats. METHODS: The methanolic pod extract of Albizzia lebbeck was administrated orally for 60 days at 50, 100 and 200 mg.kg(-1).day(-1) to male albino rats. Sperm motility and density in cauda epididymides were assessed. Biochemical and histological analysis were performed in blood samples and reproductive organs. RESULTS: Albizzia lebbeck pod extract brought about a significant decrease in the weights of testis, seminal vesicles, epdidymis and ventral prostate. The sperm motility and density were significantly reduced. There was a marked reduction in the numbers of primary spermatocytes, secondary spermatocytes and spermatids. The Sertoli cell count as well as its cross sectional surface area were significantly decreased. The Leydig cell nuclear area and the number of mature Leydig cells were also significantly decreased. The protein, glycogen and cholesterol content of the testis, the fructose in the seminal vesicles and protein in the epididymis were significantly decreased. The RBC and WBC counts, haemoglobin, haematocrit and blood sugar were within the normal range. CONCLUSION: The methanolic extract of A. lebbeck pods causes spermatogenic arrest in male albino rats.
