ABSTRACT
OBJECTIVE: To study the comparative gastroprotective effect of Luffa acutangula methanolic extract (LAM) and aqueous extract (LAW) on type II diabetes rats. METHODS: Streptozotocin (65 mg/kg, i.p.) along with nicotinamide (120 mg/kg, i.p.) was used to induce non insulin dependent diabetes mellitus (NIDDM) in rats. A daily oral dose of aspirin (200 mg/kg, i.p.) was administered for initial seven days to induce gastric ulcerations in the diabetic rats. LAM and LAW were administered orally in the doses of 100, 200 and 400 mg/kg once daily for 21 days. Glibenclamide and ranitidine were used as standards for comparing the antidiabetic and antiulcer effect respectively. RESULTS: LAM significantly (P<0.01) increased mucosal glycoprotein and antioxidant enzyme level in gastric mucosa of diabetic rats than LAW (P <0.05). LAM was efficient in reversing the delayed healing of gastric ulcer in diabetic rats close to the normal level. LAM exhibited better ulcer healing effect than glibenclamide and LAW, because of its both antihyperglycemic and mucosal defensive actions. CONCLUSIONS: Thus, LAM is proved to be a better alternative for treating gastric ulcers co-occurring with diabetes.
Subject(s)
Diabetes Mellitus, Experimental/complications , Gastric Mucosa/drug effects , Luffa , Phytotherapy , Plant Extracts/therapeutic use , Stomach Ulcer/drug therapy , Animals , Antioxidants/metabolism , Aspirin , Biomarkers/metabolism , Catalase/metabolism , Dose-Response Relationship, Drug , Drug Administration Schedule , Female , Fruit , Gastric Mucosa/metabolism , Glycoproteins/metabolism , Male , Mice , Niacinamide , Plant Extracts/pharmacology , Rats , Stomach Ulcer/chemically induced , Stomach Ulcer/complications , Streptozocin , Superoxide Dismutase , Treatment Outcome , Wound HealingABSTRACT
The ethanol extract of Curculigo orchioides was evaluated for antiasthmatic activity by using various in vitro and in vivo animal models. In vitro models like isolated goat tracheal chain preparation and isolated guinea pig ileum preparation were studied to know basic mechanism by which extract shows relaxant activity. The study showed that extract is effective against histamine-induced contraction. In isolated goat tracheal chain preparation and isolated guinea pig ileum preparation extract exhibits maximum relaxant effect (p< 0.01) against histamine at concentrations 100mug/ml and 25mug/ml respectively. Animal studies involved use of histamine induced bronchoconstriction in guinea pigs, egg albumin induced passive paw anaphylaxis in rats and haloperidol-induced catalepsy in mice. These studies showed significant (p< 0.01) protection at lower doses while further increase in the dose level showed reduced activity. Biochemical estimations in milk-induced total leukocytes count and milk-induced differential leukocyte count were also studied. In this study there was maximum increase in leucocytes and lymphocytes (99%) and maximum decrease in eosinophils up to 0% at dose 375mg/kg p.o. body weight was observed. The results of these studies indicated usefulness of ethanol extract of Curculigo orchioides in asthma.
