Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Commun Biol ; 5(1): 800, 2022 08 09.
Article in English | MEDLINE | ID: mdl-35945264

ABSTRACT

Multidomain kinases use many ways to integrate and process diverse stimuli. Here, we investigated the mechanism by which the protein tyrosine kinase 2-beta (PYK2) functions as a sensor and effector of cellular calcium influx. We show that the linker between the PYK2 kinase and FAT domains (KFL) encompasses an unusual calmodulin (CaM) binding element. PYK2 KFL is disordered and engages CaM through an ensemble of transient binding events. Calcium increases the association by promoting structural changes in CaM that expose auxiliary interaction opportunities. KFL also forms fuzzy dimers, and dimerization is enhanced by CaM binding. As a monomer, however, KFL associates with the PYK2 FERM-kinase fragment. Thus, we identify a mechanism whereby calcium influx can promote PYK2 self-association, and hence kinase-activating trans-autophosphorylation. Collectively, our findings describe a flexible protein module that expands the paradigms for CaM binding and self-association, and their use for controlling kinase activity.


Subject(s)
Calcium , Calmodulin , Calcium/metabolism , Calmodulin/metabolism , Dimerization , Focal Adhesion Kinase 2/chemistry , Focal Adhesion Kinase 2/metabolism , Phosphorylation
2.
J Biol Chem ; 290(1): 478-91, 2015 Jan 02.
Article in English | MEDLINE | ID: mdl-25391654

ABSTRACT

Focal adhesion (FA) kinase (FAK) regulates cell survival and motility by transducing signals from membrane receptors. The C-terminal FA targeting (FAT) domain of FAK fulfils multiple functions, including recruitment to FAs through paxillin binding. Phosphorylation of FAT on Tyr(925) facilitates FA disassembly and connects to the MAPK pathway through Grb2 association, but requires dissociation of the first helix (H1) of the four-helix bundle of FAT. We investigated the importance of H1 opening in cells by comparing the properties of FAK molecules containing wild-type or mutated FAT with impaired or facilitated H1 openings. These mutations did not alter the activation of FAK, but selectively affected its cellular functions, including self-association, Tyr(925) phosphorylation, paxillin binding, and FA targeting and turnover. Phosphorylation of Tyr(861), located between the kinase and FAT domains, was also enhanced by the mutation that opened the FAT bundle. Similarly phosphorylation of Ser(910) by ERK in response to bombesin was increased by FAT opening. Although FAK molecules with the mutation favoring FAT opening were poorly recruited at FAs, they efficiently restored FA turnover and cell shape in FAK-deficient cells. In contrast, the mutation preventing H1 opening markedly impaired FAK function. Our data support the biological importance of conformational dynamics of the FAT domain and its functional interactions with other parts of the molecule.


Subject(s)
Fibroblasts/metabolism , Focal Adhesion Protein-Tyrosine Kinases/chemistry , Focal Adhesions/metabolism , Amino Acid Sequence , Animals , Baculoviridae/genetics , COS Cells , Chlorocebus aethiops , Escherichia coli/genetics , Escherichia coli/metabolism , Extracellular Signal-Regulated MAP Kinases/genetics , Extracellular Signal-Regulated MAP Kinases/metabolism , Fibroblasts/cytology , Focal Adhesion Protein-Tyrosine Kinases/genetics , Focal Adhesion Protein-Tyrosine Kinases/metabolism , Focal Adhesions/ultrastructure , Gene Expression , Humans , Mice , Mice, Knockout , Models, Molecular , Molecular Sequence Data , Paxillin/genetics , Paxillin/metabolism , Protein Binding , Protein Structure, Secondary , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Sequence Alignment , Sf9 Cells , Spodoptera
3.
EMBO J ; 33(4): 356-70, 2014 Feb 18.
Article in English | MEDLINE | ID: mdl-24480479

ABSTRACT

Focal adhesion kinase (FAK) controls adhesion-dependent cell motility, survival, and proliferation. FAK has kinase-dependent and kinase-independent functions, both of which play major roles in embryogenesis and tumor invasiveness. The precise mechanisms of FAK activation are not known. Using x-ray crystallography, small angle x-ray scattering, and biochemical and functional analyses, we show that the key step for activation of FAK's kinase-dependent functions--autophosphorylation of tyrosine-397--requires site-specific dimerization of FAK. The dimers form via the association of the N-terminal FERM domain of FAK and are stabilized by an interaction between FERM and the C-terminal FAT domain. FAT binds to a basic motif on FERM that regulates co-activation and nuclear localization. FAK dimerization requires local enrichment, which occurs specifically at focal adhesions. Paxillin plays a dual role, by recruiting FAK to focal adhesions and by reinforcing the FAT:FERM interaction. Our results provide a structural and mechanistic framework to explain how FAK combines multiple stimuli into a site-specific function. The dimer interfaces we describe are promising targets for blocking FAK activation.


Subject(s)
Focal Adhesion Kinase 1/chemistry , Amino Acid Motifs , Animals , Crystallography, X-Ray , Dimerization , Enzyme Activation , Focal Adhesion Kinase 1/physiology , Focal Adhesions , HEK293 Cells , Humans , Models, Molecular , Phosphorylation , Phosphotyrosine/physiology , Protein Conformation , Protein Processing, Post-Translational , Protein Structure, Tertiary , Rats , Recombinant Fusion Proteins/chemistry , Scattering, Radiation
4.
J Cell Sci ; 120(Pt 17): 3034-44, 2007 Sep 01.
Article in English | MEDLINE | ID: mdl-17684059

ABSTRACT

Proline-rich tyrosine kinase 2 (PYK2) is a non-receptor tyrosine kinase expressed in many cell types and enriched in neurons. PYK2 is a cytoplasmic enzyme activated by increases in cytosolic free Ca(2+) through an unknown mechanism. We report that depolarization or electrical stimulation of hippocampal slices induced a rapid and transient nuclear accumulation of PYK2. Depolarization of cultured neurons or PC12 cells also triggered a Ca(2+)-dependent nuclear accumulation of PYK2, much more pronounced than that induced by blockade of nuclear export with leptomycin B. Src-family kinase activity, PYK2 autophosphorylation and kinase activity were not required for its nuclear translocation. Depolarization induced a slight decrease in PYK2 apparent molecular mass, compatible with a Ca(2+)-activated dephosphorylation. Pretreatment of PC12 cells with inhibitors of calcineurin (protein phosphatase 2B), cyclosporin A and FK506, prevented depolarization-induced nuclear translocation and tyrosine phosphorylation of PYK2. Transfection with dominant-negative and constitutively active calcineurin-A confirmed the role of calcineurin in the regulation of PYK2 tyrosine phosphorylation and nuclear accumulation. Our results show that depolarization independently induces nuclear translocation and tyrosine phosphorylation of PYK2, and that both responses require calcineurin activation. We suggest that PYK2 exerts some of its actions in the nucleus and that the effects of calcineurin inhibitors may involve PYK2 inhibition.


Subject(s)
Calcineurin/metabolism , Cell Nucleus/metabolism , Focal Adhesion Kinase 2/metabolism , Neurons/metabolism , Tyrosine/metabolism , Active Transport, Cell Nucleus , Animals , Calcineurin/genetics , Calcium/metabolism , Cells, Cultured , Electric Stimulation , Focal Adhesion Kinase 2/genetics , Hippocampus/cytology , Hippocampus/metabolism , Male , Mice , Neurons/cytology , PC12 Cells , Phosphorylation , Rats , Rats, Sprague-Dawley
5.
Biochem Biophys Res Commun ; 337(2): 641-6, 2005 Nov 18.
Article in English | MEDLINE | ID: mdl-16202977

ABSTRACT

Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases.


Subject(s)
Brain/enzymology , Focal Adhesion Kinase 2/metabolism , Focal Adhesion Protein-Tyrosine Kinases/metabolism , Nerve Tissue Proteins/metabolism , Synapses/metabolism , Animals , COS Cells , Centrifugation, Density Gradient , Chlorocebus aethiops , Enzyme Activation , Nerve Tissue Proteins/chemistry , Phosphorylation , SAP90-PSD95 Associated Proteins , Subcellular Fractions , Synapses/chemistry , Synaptosomes/metabolism , Two-Hybrid System Techniques , Tyrosine/chemistry , Tyrosine/metabolism
6.
J Biol Chem ; 278(48): 47434-40, 2003 Nov 28.
Article in English | MEDLINE | ID: mdl-14500712

ABSTRACT

Focal adhesion kinase (FAK) is a protein tyrosine kinase enriched in focal adhesions, which plays a critical role in integrin-dependent cell motility and survival. The crucial step in its activation is autophosphorylation on Tyr-397, which promotes the recruitment of several enzymes including Src family kinases and the activation of multiple signaling pathways. We found in a yeast two-hybrid screen that the N-terminal domain of FAK interacted with protein inhibitor of activated STAT1 (PIAS1). This interaction was confirmed and shown to be direct using in vitro assays. PIAS1 was co-immunoprecipitated with FAK from transfected cells and brain extracts. PIAS1 has recently been recognized as a small ubiquitin-like modifier (SUMO) ligase. In the presence of PIAS1 and SUMO-1, FAK was sumoylated in intact cells, whereas PYK2, a closely related enzyme, was not. Sumoylation occurred on Lys-152, a residue conserved in FAK during evolution. Sumoylated FAK, like PIAS1, was recovered predominantly from the nuclear fraction. Sumoylation did not require the catalytic activity or autophosphorylation of FAK. In contrast, sumoylation increased dramatically the ability of FAK to autophosphorylate in intact cells and in immune precipitate kinase assays. Endogenous FAK was sumoylated in the presence of PIAS1 and SUMO-1 independently of cell adhesion, and autophosphorylation of sumoylated FAK was persistently increased in suspended cells. These observations show that sumoylation controls the activity of a protein kinase and suggest that FAK may play a novel role in signaling between the plasma membrane and the nucleus.


Subject(s)
Protein-Tyrosine Kinases/metabolism , Proteins/physiology , Amino Acid Sequence , Animals , Brain/metabolism , COS Cells , Catalysis , Cell Adhesion , Cell Membrane/metabolism , Cell Movement , Cell Nucleus/metabolism , Cell Survival , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Gene Library , Glutathione Transferase/metabolism , Humans , Lysine/chemistry , Mice , Molecular Sequence Data , NIH 3T3 Cells , Phosphorylation , Precipitin Tests , Protein Binding , Protein Inhibitors of Activated STAT , Protein Structure, Tertiary , Proteins/metabolism , Rats , Sequence Homology, Amino Acid , Signal Transduction , Transfection , Two-Hybrid System Techniques , Tyrosine/metabolism
7.
Mol Cell Biol ; 22(22): 7731-43, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12391143

ABSTRACT

Focal adhesion kinase (FAK) is activated following integrin engagement or stimulation of transmembrane receptors. Autophosphorylation of FAK on Tyr-397 is a critical event, allowing binding of Src family kinases and activation of signal transduction pathways. Tissue-specific alternative splicing generates several isoforms of FAK with different autophosphorylation rates. Despite its importance, the mechanisms of FAK autophosphorylation and the basis for differences between isoforms are not known. We addressed these questions using isoforms of FAK expressed in brain. Autophosphorylation of FAK(+), which is identical to that of "standard" FAK, was intermolecular in transfected cells, although it did not involve the formation of stable multimeric complexes. Coumermycin-induced dimerization of gyrase B-FAK(+) chimeras triggered autophosphorylation of Tyr-397. This was independent of cell adhesion but required the C terminus of the protein. In contrast, the elevated autophosphorylation of FAK(+6,7), the major neuronal splice isoform, was not accounted for by transphosphorylation. Specifically designed immune precipitate kinase assays confirmed that autophosphorylation of FAK(+) was intermolecular, whereas autophosphorylation of FAK(+6,7) or FAK(+7) was predominantly intramolecular and insensitive to the inhibitory effects of the N-terminal domain. Our results clarify the mechanisms of FAK activation and show how alternative splicing can dramatically alter the mechanism of autophosphorylation of a protein kinase.


Subject(s)
Alternative Splicing , Isoenzymes/metabolism , Protein-Tyrosine Kinases/metabolism , Aminocoumarins , Animals , Brain/enzymology , COS Cells , Coumarins/metabolism , DNA Gyrase/genetics , DNA Gyrase/metabolism , Dimerization , Enzyme Inhibitors/metabolism , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Isoenzymes/genetics , Models, Biological , Mutagenesis, Site-Directed , Novobiocin/metabolism , Peptides/genetics , Peptides/metabolism , Phosphorylation , Protein-Tyrosine Kinases/genetics , Rats , Signal Transduction/physiology , Topoisomerase II Inhibitors , Tyrosine/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...