Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 109
Filter
1.
J Adv Periodontol Implant Dent ; 16(1): 22-29, 2024.
Article in English | MEDLINE | ID: mdl-39027209

ABSTRACT

Background: Xenograft bone substitutes can be obtained from different animals and processed using various methods. The present in vivo study evaluated bone regeneration after using three types of xenografts with different sources in critical-sized bone defects in rabbit calvaria. Methods: Four 8-mm defects were created in calvaria of 14 New Zealand and white male rabbits. Three out of four defects were filled with xenografts of bovine, camel, and ostrich sources. The fourth defect was left unfilled as the control group. Seven rabbits were sacrificed after eight weeks and seven others after 12 weeks. Micro-CT imaging and histologic evaluation were further performed on dissected calvarias. Results: After 8 and 12 weeks, the highest and lowest percentages of new bone formation were observed in the camel (27.71% and 41.92%) and control (11.33% and 15.96%) groups, respectively. In the case of residual material, the ostrich group had the most value after eight weeks (53%), while after 12 weeks, it was highest in the camel group (37%). Micro-CT findings were consistent with histologic results. Conclusion: Although all three xenografts can be good choices for treating bone defects, camel-sourced xenograft seemed to be better than the other two groups. The origin and processing procedures of xenografts affected their final characteristics, which should be considered for clinical use.

2.
Article in English | MEDLINE | ID: mdl-39027212

ABSTRACT

Background: The use of bone graft materials has significantly increased. Given the inherent variations in structure and functionality between different grafting materials, this evaluated and compared the physical attributes of antler and bovine femur bone substitutes. Methods: In the present in vitro investigation, the surface morphological architecture of the two bone substitutes with different origins was assessed through scanning electron microscopy. Furthermore, the Brunauer-Emmett-Teller (BET) technique was employed to measure the porosity, specific surface area (SSA), and pore morphology. Results: Scanning electron microscopy observations indicated that the surface of the bovine particles appeared smoother, while the antler particles exhibited a rougher surface texture. The BET analysis revealed that both samples exhibited identical pore morphology. The SSA was 15.974 m2/g in the antler particles compared with 18.404 m2/g in the bovine sample. The total porosity volume in the antler and bovine femur bone substitutes were 0.2172 cm3/g and 0.2918 cm3/g, respectively. Additionally, the antler particles had a porosity percentage of 40%, whereas the bovine femur bone substitute showed a porosity percentage of 43.5%. Conclusion: Based on the results of this study, it seems that the two samples of bone grafting materials have comparable physical structures.

3.
Arch Oral Biol ; 166: 106034, 2024 Jun 27.
Article in English | MEDLINE | ID: mdl-38943857

ABSTRACT

OBJECTIVE: This systematic review aims to evaluate existing evidence to investigate the therapeutic efficacy of M2 macrophage-derived exosomes in bone regeneration. DESIGN: A comprehensive search between 2020 and 2024 across PubMed, Web of Science, and Scopus was conducted using a defined search strategy to identify relevant studies regarding the following question: "What is the impact of M2 macrophage-derived exosomes on bone regeneration?". Controlled in vitro and in vivo studies were included in this study. The SYRCLE tool was used to evaluate the risk of bias in the included animal studies. RESULTS: This review included 20 studies published. Seven studies were selected for only in vitro analysis, whereas 13 studies underwent both in vitro and in vivo analyses. The in vivo studies employed animal models, including 163 C57BL6 mice and 73 Sprague-Dawley rats. Exosomes derived from M2 macrophages were discovered to be efficacious in promoting bone regeneration and vascularization in animal models of bone defects. These effects were primarily confirmed through morphological and histological assessments. This remarkable outcome is attributed to the regulation of multiple signaling pathways, as evidenced by the findings of 11 studies investigating the involvement of miRNAs in this intricate process. In addition, in vitro studies observed positive effects on cell proliferation, migration, osteogenesis, and angiogenesis. Heterogeneity in study methods hinders direct comparison of results across studies. CONCLUSION: M2 macrophage-derived exosomes demonstrate remarkable potential for promoting bone regeneration. Further research optimizing their application and elucidating the underlying mechanisms can pave the way for clinical translation.

4.
J Oral Implantol ; 2024 May 08.
Article in English | MEDLINE | ID: mdl-38716583

ABSTRACT

The present study aims to evaluate the trends of oral implant-related research in design and topics between the range of 2016 to the end of 2022. The electronic search was conducted in MEDLINE via Pubmed. Papers published in Clinical Oral Implant Research, Clinical Implant Dentistry and Related Research, International Journal of Oral Implantology and previously, European Journal of Oral Implantology, International Journal of Oral and Maxillofacial Implants, and Journal of Oral Implantology, Between January 1, 2016, to December 30, 2022, were retrieved. Articles were classified according to their study design and major subjects. The Joinpoint regression model was used to determine changes in the trends of study designs and topics. Statistical significance was defined as a p-value < 0.05. A total of 3,382 articles were analyzed in this study. In the specified period, in vivo, prospective cohort, retrospective case-control, randomized clinical studies, in the design; and prosthetic complications, peri-implant hard tissue studies, in the topic, experienced a significant decreasing pattern in the number of published articles as well as the total number of articles. Case reports and series, retrospective cohort, non-randomized clinical studies, in the design; and outcomes of implant-related treatment plans, immediate implant placement, peri- implantitis, in the topic, experienced a significant decreasing pattern followed by a significant increasing pattern with a turning point between 2017 to 2020. Considering the limitations of this scientific topical trends analysis, it can be concluded that the recent pandemic affected the research path in oral implantology in many ways.

5.
Head Face Med ; 20(1): 23, 2024 Apr 02.
Article in English | MEDLINE | ID: mdl-38566169

ABSTRACT

BACKGROUND: Transgingival probing is conventionally used for gingival thickness (GT) measurement. However, invasiveness is a major drawback of transgingival probing. Thus, researchers have been in search of alternative methods for measurement of GT. This study compared the clinical efficacy of intraoral ultrasonography and transgingival probing for measurement of GT in different biotypes. MATERIALS AND METHODS: This clinical trial was conducted on 34 patients requiring crown lengthening surgery. GT was measured at 40 points with 2- and 4-mm distances from the free gingival margin (FGM) of anterior and premolar teeth of both jaws in each patient by an intraoral ultrasound probe. For measurement of GT by the transgingival probing method, infiltration anesthesia was induced, and a #25 finger spreader (25 mm) was vertically inserted into the soft tissue until contacting bone. The inserted length was measured by a digital caliper with 0.01 mm accuracy. All measurements were made by an operator with high reliability under the supervision of a radiologist. Data were analyzed by t-test, Power and Effect Size formula, and intraclass correlation coefficient (ICC). RESULTS: The two methods were significantly different in measurement of GT in both thick and thin biotypes at 2- and 4-mm distances (P < 0.001). The two methods had a significant difference in both the mandible (P < 0.001) and maxilla (P < 0.001) and in both the anterior (P < 0.003) and premolar (P < 0.003) regions. Although the difference was statistically significant in t-tests, the power and effect formula proved it to be clinically insignificant. Also, the ICC of the two methods revealed excellent agreement. CONCLUSION: The results showed optimal agreement of ultrasound and transgingival probing for measurement of GT. TRIAL REGISTRATION: The study was approved by the ethics committee of Shahid Beheshti University of Medical Sciences on 2021-12-28 (IR.SBMU.DRC.REC.1400.138) and registered in the Iranian Registry of Clinical Trials on 2022-03-14 (IRCT20211229053566N1).


Subject(s)
Gingiva , Maxilla , Humans , Bicuspid , Gingiva/diagnostic imaging , Iran , Maxilla/diagnostic imaging , Maxilla/surgery , Reproducibility of Results , Treatment Outcome , Ultrasonography
6.
Gen Dent ; 72(3): 61-66, 2024.
Article in English | MEDLINE | ID: mdl-38640008

ABSTRACT

In addition to the proper selection of techniques, appropriate treatment sequencing and prioritization are prerequisites for successful periodontal and implant procedures. The aim of this study was to provide evidence-based time frames for various procedures pertaining to periodontal and implant treatment. A literature review was conducted to collect data on tissue healing; in areas in which data were lacking, the viewpoints of experienced clinicians were solicited to establish a consensus. This review reports recommended time frames for the healing processes associated with surgical crown-lengthening procedures (both functional and esthetic), fresh socket management, alveolar ridge management, soft tissue management, sinus floor augmentation, implant loading, and peri-implant defect management.


Subject(s)
Alveolar Ridge Augmentation , Dental Implants , Sinus Floor Augmentation , Humans , Alveolar Ridge Augmentation/methods , Dental Implantation, Endosseous/methods , Time Management
7.
J Long Term Eff Med Implants ; 34(2): 17-27, 2024.
Article in English | MEDLINE | ID: mdl-38305367

ABSTRACT

This study aimed to compare the volume and quality of the newly formed bone following application of two types of xenografts and one synthetic material in bone defects in rabbit calvaria from histological and micro-CT aspects. Four 8-mm defects were created in 12 rabbit calvaria. Three defects were filled with bone substitutes and one was left unfilled as the control group. The newly formed bone was evaluated histologically and also by micro-CT at 8 and 12 weeks after the intervention. The percentage of osteogenesis was comparable in histomor-phometric assessment and micro-CT. Histological analysis showed that the percentage of the newly formed bone was 10.92 ± 5.17%, 14.70 ± 11.02%, 11.47 ± 7.04%, and 9.45 ± 5.18% in groups bovine 1, bovine 2, synthetic, and negative control, respectively after 8 weeks. These values were 33.70 ± 11.48%, 26.30 ± 18.05%, 22.92 ± 6.30%, and 14.82 ± 8.59%, respectively at 12 weeks. The difference in the percentage of the new bone formation at 8 and 12 weeks was not significant in any group (P > 0.05) except for bovine 1 group (P < 0.05). Micro-CT confirmed new bone formation in all groups but according to the micro-CT results, the difference between the control and other groups was significant in this respect (P < 0.05). All bone substitutes enhanced new bone formation compared with the control group. Micro-CT assessment yielded more accurate and different results compared with histological assessment.


Subject(s)
Bone Substitutes , Osteogenesis , Humans , Animals , Cattle , Rabbits , Bone Substitutes/pharmacology , Bone Regeneration , Skull/diagnostic imaging , Skull/surgery
8.
J Esthet Restor Dent ; 36(4): 548-554, 2024 Apr.
Article in English | MEDLINE | ID: mdl-37850403

ABSTRACT

OBJECTIVE: The purpose of this study was to assess the feasibility of phenotype modification in orthodontic patients using combined bone and soft tissue grafting substitutes. CLINICAL CONSIDERATION: The surgical procedure was conducted on 18 patients (3 males, 15 females). Periodontal phenotype modification was conducted using demineralized freeze-dried bone allograft and a xenogeneic collagen matrix. The following parameters were recorded for each tooth at baseline and 12-month follow-up: O'Leary plaque index (PI), probing depth (PD), bleeding on probing (BOP), gingival thickness (GT), keratinized tissue width (KTW), gingival recession (GR), and vestibular depth (VD). The results showed a statistically significant increase in GT (2.02 ± 0.39 mm), KTW (1.11 ± 0.82 mm), and VD (0.18 ± 1.16 mm) (p < 0.05). GR was also significantly decreased (1.02 ± 0.99 mm) (p < 0.05). CONCLUSION: Within the limitation of this study, the proposed approach enhanced the periodontal condition in orthodontic patients. However, further studies with a larger sample size are needed to ensure long-term stability. CLINICAL SIGNIFICANCE: Hard and soft tissue conditions have paramount importance for long-term periodontal stability. Phenotype modification in orthodontic patients can diminish the probability of adverse consequences and result in optimal esthetic outcomes. The proposed technique using combined bone and soft tissue substitutes indicated promising results and could be recommended in orthodontic patients with thin periodontal phenotypes.


Subject(s)
Gingiva , Gingival Recession , Male , Female , Humans , Follow-Up Studies , Periodontal Pocket/surgery , Periodontal Attachment Loss/surgery , Gingival Recession/surgery , Phenotype
9.
Clin Oral Investig ; 27(12): 7417-7423, 2023 Dec.
Article in English | MEDLINE | ID: mdl-37848583

ABSTRACT

OBJECTIVES: The current study aimed to investigate the association of matrix metalloproteinase- (MMP-) 1, -2, -3, -7, and -13 gene polymorphisms with chronic periodontitis (CP) in an Iranian population. MATERIALS AND METHODS: In this case-control study, 87 subjects with CP and 89 periodontally healthy subjects were allocated to case and control groups, respectively. Subjects' venous blood samples (5 cc) were collected, and DNA extraction was performed. A spectrophotometer was utilized to assess the concentration of extracted DNAs. The desired gene polymorphisms were examined using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) followed by electrophoresis. Statistical analyses were done using the Pearson Chi-Square test, odds ratio, and t-Test using SPSS Version 28. RESULTS: The MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 gene polymorphisms significantly differed between case and control groups (PV = 0.019, 0.007, and 0.028, respectively). In contrast, the gene polymorphisms of MMP-2 (-1306 C/T) rs243865 and MMP-13 (-77 A/G) rs2252070 did not make a significant difference. Regarding allele frequencies, the presence of the 2G allele in the MMP-1 (-1607) rs1799750 genotype increased the CP susceptibility significantly, while subjects with the 6A allele in their MMP-3 (-1171) rs3025058 genotype showed significantly lower susceptibility to CP (PV = 0.008 and < 0.001, respectively). CONCLUSION: In the studied population, gene polymorphisms in the DNA sequences of MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 may have impacts on CP incidence. CLINICAL RELEVANCE: Clinicians should be cautious about the association between MMP-1, MMP-3, and MMP-7 gene polymorphisms and the incidence of chronic periodontitis during periodontal treatment planning.


Subject(s)
Chronic Periodontitis , Humans , Chronic Periodontitis/genetics , Matrix Metalloproteinase 1/genetics , Matrix Metalloproteinase 3/genetics , Matrix Metalloproteinase 7/genetics , Case-Control Studies , Iran , Genetic Predisposition to Disease , Polymorphism, Genetic/genetics , Gene Frequency , Genotype , Alleles , Polymorphism, Single Nucleotide
10.
J Long Term Eff Med Implants ; 33(4): 77-91, 2023.
Article in English | MEDLINE | ID: mdl-37522589

ABSTRACT

Considering the widespread use of dental implants, a precise definition for peri-implant tissue health and an appropriate classification for peri-implant diseases are imperative for researchers and dental clinicians. However, absence of a unanimous definition and an efficient classification system has created controversies in the published reports regarding the epidemiology and prevalence of peri-implant diseases. Moreover, lack of a standard system for differentiation of different grades of peri-implantitis further complicates the interpretation of reports regarding the diagnosis prevalence, treatment, and outcome of such conditions, and highlights the need for a classification system based on the severity of disease. Almost all of the currently available classification systems focus on the assessment of health or disease status of the tissues around loaded implants. The purpose of the present study is to propose a classification/scoring system for peri-implant tissue health before and after prosthetic loading. This grading system can aid the researchers and dental clinicians in assessment of peri-implant tissue condition both before and after prosthetic loading of dental implants.

11.
J Prosthet Dent ; 2023 Feb 11.
Article in English | MEDLINE | ID: mdl-36781339

ABSTRACT

STATEMENT OF PROBLEM: Whether placing implants to replace each missing tooth or using implant-supported fixed partial dentures provides better outcomes is unclear. PURPOSE: The purpose of this systematic review and meta-analysis was to assess the success and survival rates of implants supporting single-unit and multiunit fixed prostheses by using conventional protocols for placement and loading in short- and long-term follow-ups. MATERIAL AND METHODS: An electronic search was conducted in PubMed, Scopus, and Web of Science for studies published up to March 17, 2022. Comparative studies that reported the success or survival rates of both single-unit and splinted multiunit prostheses were considered for qualitative and quantitative analyses. RESULTS: A total of 68 publications comprising 11 271 implants were included. Compared with the single prostheses, the splinted multiunit group showed no significant differences in implant success rates in the short-term (risk difference=-0.004; 95% confidence interval (CI)=-0.033 to 0.025; P=.780) and long-term (risk difference=0.003; 95% confidence interval (CI)=-0.029 to 0.034; P=.874) follow-ups. Significant statistical differences were also not found in terms of the survival rates of the 2 groups (short-term risk difference=-0.004; 95% CI=-0.031 to 0.023; P=.779, long-term risk difference=-0.002; 95% CI=-0.029 to 0.025; P=.887). CONCLUSIONS: Implants supporting single-unit or splinted multiunit prostheses seem to be a predictable treatment in terms of survival and success over short and long periods. Nonetheless, it seems that cantilever and nonsplinted multiunit prostheses should be used with more caution.

12.
J Prosthet Dent ; 130(3): 307-317, 2023 Sep.
Article in English | MEDLINE | ID: mdl-34772483

ABSTRACT

STATEMENT OF PROBLEM: Immediate implant placement provides a popular therapeutic option. However, compromised sockets may jeopardize the treatment outcome. PURPOSE: The purpose of this systematic review and meta-analysis was to investigate the treatment outcome in terms of the implant survival rate and success parameters of immediate implant placement in compromised extraction sockets. MATERIAL AND METHODS: An electronic search was conducted in PubMed, Embase, Cochrane Library, and ISI Web of Science up to January 2021. Studies evaluating implant survival rate and main success parameters were included for a qualitative and quantitative analysis (risk ratio and mean difference). RESULTS: In total, 43 studies with analysis of 4825 sockets were included. Compared with the noncompromised sockets, the compromised group showed no significant differences in implant survival rates (risk ratio=0.992; 95% confidence interval (CI)=0.979 to 1.005; P=.246). No significant statistical differences were found in marginal bone level at ≤12 months (mean difference [MD]=0.033; 95% CI=-0.012 to 0.078; P=.154) or esthetic parameters. CONCLUSIONS: Immediate implant placement in compromised sites does not appear to decrease the survival and success rates. However, randomized clinical trials with large sample sizes should be conducted to draw a definite conclusion about the efficacy and safety of this treatment protocol in compromised sockets.


Subject(s)
Dental Implants , Immediate Dental Implant Loading , Dental Implantation, Endosseous/methods , Tooth Socket/surgery , Esthetics, Dental , Immediate Dental Implant Loading/methods
13.
J Indian Soc Periodontol ; 26(5): 440-445, 2022.
Article in English | MEDLINE | ID: mdl-36339380

ABSTRACT

Aims: This study aimed to compare the biological reactions of dental pulp stem cells (DPSCs) cultured on new xenograft bone substitutes derived from camel and bovine bones. Materials and Methods: DPSCs were cultured and placed on different xenograft materials including Bone Plus (bovine), Camel Bone, and demineralized bovine bone matrix. The viability and proliferation of cells were evaluated by the methyl thiazolyl tetrazolium assay after 24, 48, and 72 h. The alkaline phosphatase (ALP) test and Alizarin red staining were performed at 7 and 21 days to assess the osteoblastic differentiation of cells. Osteocalcin (OCN) gene expression was evaluated qualitatively at 3-, 7- and 14-days using polymerase chain reaction (PCR). Semi-quantitative PCR was also performed using ImageJ software. Data were analyzed by ANOVA and Tukey's honestly significant difference test. Results: The cell proliferation rate was significantly different among the three xenograft bone substitutes at 24-, 48- and 72 h (P < 0.05). The ALP activity of DPSCs in all three xenograft bone substitute groups was greater than that in the control group (P < 0.05). Alizarin red staining showed no significant difference in the formation of calcified nodules among the groups. Qualitative and semi-quantitative PCR displayed that the expression of OCN gene in the Camel Bone and Bone Plus groups was higher than that in the demineralized bovine bone matrix group. Conclusions: The Camel Bone xenograft caused a high proliferation rate and optimal osteogenic differentiation of DPSCs qualitatively and semi-quantitatively in vitro. Further studies are required on this xenograft bone substitute.

14.
Gen Dent ; 70(6): 71-77, 2022.
Article in English | MEDLINE | ID: mdl-36288079

ABSTRACT

Many of the criteria commonly considered in treatment planning for severely damaged teeth are well known to clinicians. However, a systematic approach to decision-making is lacking. The purpose of this article is to introduce a quantitative systematic risk assessment scoring system (RASS) to determine the long-term prognosis for severely damaged teeth based on several important factors. Before any treatment decision is made, the dentist should take into account the role of systemic parameters such as the medical condition of the patient, smoking status, psychological factors, patient expectations with regard to the duration and overall cost of treatment, and periodontal health. In the assessment of local factors, emphasis should be placed on 5 parameters: crown to root ratio, root condition, risk of furcation involvement, complexity of the final restoration, and esthetic results. In the proposed RASS, each of these parameters is ranked using 4 color-coded levels of risk: optimal (green), favorable (blue), unfavorable (yellow), or hopeless (red). The presence of even 1 parameter in the red zone is sufficient to consider tooth extraction. If no parameter is in the red zone, the final decision should be made after the possible effects of all of the clinical conditions are weighed and the overall risk of treatment failure is determined. Clinical decision-making with regard to the preservation or extraction of severely damaged teeth is a challenging, multifactorial process. The RASS introduced in this article focuses on 5 main factors to simplify and organize the decision-making process; however, many other parameters may affect the final treatment decision. Moreover, no decision-making system can be definitively applied to all clinical scenarios, and the entire process depends on the knowledge, experience, and expertise of the clinician.


Subject(s)
Patient Care Planning , Tooth , Humans , Crowns , Prognosis , Tooth/pathology , Tooth Crown , Dental Implants , Risk Assessment , Decision Making , Crown Lengthening
15.
J Adv Periodontol Implant Dent ; 14(1): 20-25, 2022.
Article in English | MEDLINE | ID: mdl-35919451

ABSTRACT

Background: The maxillary palatine process (MPP) is an excellent source of autogenous bone transplants for anterior maxillary reconstruction. This research aimed to determine the quantity and quality of accessible MPP as a donor location. Methods: Cone-beam computed tomography (CBCT) scans of patients referred to the School of Dentistry were evaluated by a certified examiner. The harvestable MPP was defined as the space between the distal surfaces of maxillary first premolars. OnDemand 3D Imaging software was used to determine bone properties. SPSS software was used to investigate the following variables: Bone characteristics are correlated with age and gender, accessible volume, and palatal width and height. P<0.05 was defined as the level of statistical significance. Results: This study was performed on CBCT scans of 81 subjects (41 females and 40 males). MPP volume and palatal heights were 1.33±0.53 cm3 and 20.86±5.51 mm, respectively. Maximum bone density was observed around lateral incisors. Palatal width was 33.81±2.42 mm between canines and 41.81±2.66 mm between premolars. The MPP volume was significantly greater in males (P<0.001). Additionally, there was a positive correlation between the volume and palatal width (P<0.05). Conclusion: Within the constraints of this research, there is a limited supply of MMP accessible for use as a graft source, and it is best suited for treating localized bone lesions. The favorable link between palatal breadth and harvestable volume aids the surgeon in estimating the quantity of bone accessible during the first evaluation.

16.
Lasers Med Sci ; 37(8): 3259-3268, 2022 Oct.
Article in English | MEDLINE | ID: mdl-35907129

ABSTRACT

Decontamination of implant surfaces is important to the treatment of peri-implantitis. Er:YAG laser and air-powder abrasion system are regarded as the most effective means of decontamination of implant surfaces. The aim of this in vitro study was to compare the activity of human dental pulp stem cells (hDPSCs) cultured on decontaminated sandblasted titanium discs using Er:YAG laser irradiation and air-powder abrasion. Forty-five titanium discs were contaminated with Escherichia coli (E. coli) bacteria and fifteen titanium discs served as sterile control groups. Thirty contaminated titanium discs were decontaminated with Er:YAG laser or air-powder abrasion system and fifteen contaminated discs were used as contaminated control group. Afterwards, hDPSCs were seeded on all sixty experimental titanium discs. The effects of two decontamination tools on hDPSCs viability were evaluated by MTT assay. Alkaline phosphatase (ALP) activity assay, quantitative real-time PCR analysis and alizarin red staining method were performed to assess hDPSCs osteogenic differentiation. Scanning microscope electron (SEM) was also used to evaluate the effects of two different decontaminated methods on cellular morphology. Our study showed that decontamination using Er:YAG laser caused maximum cell viability. However, the ALP activity was not different in laser and air-abrasion groups. The significant expression of an osteoblastic marker and stronger Alizarin red staining were observed in laser irradiation groups. In addition, SEM observation indicated that grown cells were more stretched and more filopodia in Er:YAG-treated discs. In the present study, Er:YAG laser and air-powder abrasion improved the activity of the cells cultured on the decontaminated titanium discs. However, in comparison with air-powder abrasion, Er:YAG laser was more effective.


Subject(s)
Dental Implants , Lasers, Solid-State , Alkaline Phosphatase , Anthraquinones , Dental Pulp , Escherichia coli , Humans , Lasers, Solid-State/therapeutic use , Microscopy, Electron, Scanning , Osteogenesis , Powders , Stem Cells , Surface Properties , Titanium/pharmacology
17.
J Long Term Eff Med Implants ; 32(2): 31-33, 2022.
Article in English | MEDLINE | ID: mdl-35695624

ABSTRACT

OBJECTIVE: Consenting to a dental implant treatment plan may be a challenging decision for many patients, and they may prefer to consult their family and friends before making the final decision on this matter. The purpose of this preliminary report was to assess the role of family and friends, as the consultees, in consenting to a dental implant treatment plan by patients. MATERIALS AND METHODS: A total of 106 patients with dental implants were questioned about whether they made the final decision regarding their dental implant treatment by themselves or they consulted their family and friends prior to finalizing their decision. RESULTS: The majority of men (52.1%) reported making this decision by themselves and based on their own personal opinion, while most women (48.2%) reported making the decision after consulting their children. CONCLUSIONS: Within the limitations of this preliminary report, it appears that women are more willing to consult their children and friends on this matter compared with men. Also, men mostly consult their wives in making such a decision while women pay more attention to the opinion of their children in this respect.


Subject(s)
Dental Implants , Child , Decision Making , Female , Friends , Humans , Iran , Male
18.
J Long Term Eff Med Implants ; 32(2): 51-59, 2022.
Article in English | MEDLINE | ID: mdl-35695627

ABSTRACT

Successful bone regeneration often requires induction by signaling molecules. Enamel matrix derivative (EMD) is said to enhance initial phases of healing. N-acetyl cysteine (NAC) is a molecule assumed to enhance osteogenesis and induce osteoblastic differentiation. This study sought to compare effects of EMD and NAC on proliferation, mineralization, and enzymatic activity of dental pulp mesenchymal stem cells (DPSCs). DPSCs were cultured on mineralized bone allograft (MBA) powder. After 24 hours, EMD in concentrations of 10, 50, and 100 µg/mL and NAC in 5 mM concentration were added. Methyl thiazolyl tetrazolium (MTT) assay was used for cell proliferation assessment at 1, 2, and 3 days. Osteoblastic differentiation of DPSCs was evaluated at 30 days, by alizarin red staining and assessment of alkaline phosphatase (ALP) activity. Both EMD and NAC caused time-dependent reduction of cell proliferation compared with the negative control. Maximum proliferation of DPSCs was observed in the 10 µg/mL EMD group at all time points, whereas NAC caused higher ALP activity and mineralization of DPSCs compared with EMD. In vitro application of NAC, as a signaling molecule, may effectively enhance bone regeneration by the induction of mineralization and enzymatic activity, despite the resultant reduction in cell proliferation rate.


Subject(s)
Acetylcysteine , Osteogenesis , Acetylcysteine/pharmacology , Cell Differentiation , Cell Proliferation , Cells, Cultured , Dental Pulp , Humans , Stem Cells
19.
J Lasers Med Sci ; 13: e1, 2022.
Article in English | MEDLINE | ID: mdl-35642237

ABSTRACT

Introduction: Stem cell activities have different effects on tissue response and its outcomes. Low-level laser therapy (LLLT) can be considered a trigger to modify stem cell activities. The objective of the present experimental investigation was to study the effects of two protocols of LLLT on the proliferation and differentiation of human dental pulp stem cells (hDPSCs) cultured on sandblasted titanium discs. Methods: Cells obtained from human dental pulp were seeded/cultured on titanium discs and were set in 2 main groups: (i) Radiated cells using the gallium-aluminium-arsenide (GaAlAs) diode laser at a continuous wavelength of 808 nm at 3 J/cm2 for 12 sec or 5 J/cm2 for 20 seconds, and (ii) Non-irradiated cells serving as control groups. The impact of LLLTs on hDPSC-proliferation and viability was investigated using the MTT assay after 24, 72 and 96 hours. The alkaline phosphatase activity was studied with p-nitrophenylphosphate after 14 and 28 days. The ability of hDPSCs to express osteocalcin was investigated using real-time polymerase chain reaction after 28 days, while their attachment was observed under a scanning electron microscope (SEM) after 14 and 28 days. Results: Our study showed that LLLTs caused maximum cell proliferation in 96 hours (P<0.001) with 3 J/cm2 resulting in a higher proliferation rate. The highest activity of alkaline phosphatase and osteocalcin expression was observed in the laser radiation groups after 28 days. Conclusion: The outcomes of the current study showed that cultured hDPSCs on sandblasted titanium discs had a tendency towards increased cellular activity in response to LLLTs. Thus, LLLTs could regulate the activities of hDPSCs on bone repair surrounding the sandblasted titanium discs.

20.
J Maxillofac Oral Surg ; 21(2): 548-556, 2022 Jun.
Article in English | MEDLINE | ID: mdl-35712417

ABSTRACT

Objective: The aim of this study was to evaluate the clinical and radiographic outcomes of the "vertically expander screw" (VES) technique as a novel approach for maxillary sinus floor elevation and simultaneous implant placement. Materials and Methods: Forty-four patients (26 females, 18 males) received 71 implants with simultaneous transalveolar sinus floor elevation via the VES technique. The threaded bone expanders were used to elevate the sinus floor as well as bone expansion. The patients were followed-up on a regular basis, and final clinical and radiographic examinations were performed at least 24 months following functional loading. Statistical analysis was conducted using the Chi-square test, the correlation coefficient, and the independent t test (p < 0.05, 95% confidence interval). Results: All implants showed successful osseointegration. The mean marginal bone loss (MBL; 0.38 ± 0.75 mm) at the site of premolars was not significantly different from the value at the site of molars (0.17 ± 0.50 mm). The mean intrasinus bone gain (IBG) was not significantly different (3.47 ± 1.22 mm and 4.11 ± 1.67 mm, respectively; p = 0.92). Evaluation of implant success index (ISI) score revealed no difference between the premolars and molars (p = 0.12). Conclusion: Despite the limitations of the present study, it seems that the VES technique and simultaneous implant placement in carefully selected cases may result in promising outcomes. Clinical Relevance: Insufficient alveolar bone height and poor bone quality often complicate implant placement in the edentulous posterior maxilla. The VES technique and simultaneous implant placement can bring about favorable clinical and radiographic outcomes and high survival rate in carefully selected cases.

SELECTION OF CITATIONS
SEARCH DETAIL
...