Copper(II) phthalocyanine as an electrocatalytic electrode for cathodic detection of urinary tryptophan.

Herein, we introduce a novel method for tryptophan detection via a reduction reaction facilitated by its interaction with a copper(II) phthalocyanine (CuPc) electrocatalytic electrode. This method addresses challenges associated with the susceptibility of the oxidation response to interference from various species when measuring tryptophan in bodily fluids. The reduction currents exhibit a linear increase with tryptophan concentrations in two ranges: 0.0013-0.10 mM and 0.10-1.20 mM, with the sensitivities of 14.7 ± 0.5 µA mM-1 and 3.5 ± 0.1 µA mM-1, respectively. The limit of detection (LOD, 3SB/m) is determined to be 0.39 µM. The sensor exhibits excellent reproducibility, with the relative standard deviation of <5%. Application of the sensor to authentic urine samples yields a % recovery of 101 ± 4%.

Electrochemical detection of creatinine: exploiting copper(ii) complexes at Pt microelectrode arrays.

This work develops a rapid and highly sensitive electrochemical sensor for creatinine detection at platinum microelectrode arrays (Pt-MEA). Copper(ii) ions are introduced to form the electroactive creatinine complex, which is then detected at Pt-MEA through a direct reduction reaction. Electrochemical behaviors of the creatinine complex are also explored at Pt macrodisc and microdisc electrodes in comparison with Pt-MEA. At the Pt-MEA, the linear range, sensitivity, and limit of detection of creatinine are determined to be 0.00-5.00 mM, 5401 ± 99 A m-2 M-1, and 0.059 mM (3SB/m), respectively. Notably, the Pt-MEA requires only 10 µL of sample and allows direct measurement of creatinine in synthetic urine with 97.39 ± 4.78% recovery.

Electrodeposited copper nanoparticles for creatinine detection via the in situ formation of copper-creatinine complexes.

Creatinine is an important biomarker of kidney diseases. In this work, a fast and facile electrochemical sensor was developed for creatinine detection based on the use of copper nanoparticle-modified screen-printed electrodes. The copper electrodes were prepared by simple electrodeposition of Cu2+ (aq). The electrochemically inactive creatinine was detected reductively via the in situ formation of copper-creatinine complexes. Two linear detection ranges, 0.28-3.0 mM and 3.0-20.0 mM, were achieved using differential pulse voltammetry, with the sensitivities of 0.824 ± 0.053 µA mM-1 and 0.132 ± 0.003 µA mM-1, respectively. The limit of detection was determined to be 0.084 mM. The sensor was validated in synthetic urine samples to yield 99.3% recovery (%RSD = 2.8), demonstrating high tolerance to possible interfering species. Finally, the stability of creatinine and its degradation kinetics at different temperatures were evaluated using our developed sensor. The loss of creatinine was found to be a first-order reaction with the activation energy of 64.7 kJ mol-1.

Microporous carbon for fast and simple electrochemical detection of imidacloprid insecticide in fruit and water samples.

Herein, a fast and sensitive electrochemical sensor was developed for imidacloprid detection using low-cost disposable microporous carbon screen-printed electrodes. The electrochemical behaviour of imidacloprid at the microporous material was investigated in detail. The developed sensor allowed imidacloprid detection in the linear range of 0.00-1.00 mM with a sensitivity of 14.43 ± 0.42 µA mM-1 and a detection limit of 2.54 µM (3s B/m). The sensor showed excellent selectivity and high tolerance to possible interference from other tested insecticides and ions. Excellent repeatability (3.42%, n = 3) and reproducibility (2.23%, n = 3) were demonstrated. Application of the sensor in various fruit and water samples without any treatment showed 96.2-103.0% recoveries. The developed sensor further revealed that the most effective method for removing imidacloprid residue from fruit samples was via washing with a mixture of 5% w/v NaCl and 5% w/v bicarbonate at 40 °C.